Transgelin 2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Transgelin 2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

18

Reviews (1)

Cat no : 10234-2-AP

Synonyms

SM22 beta, SM22beta, TAGLN2, Transgelin 2

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Validation Data Gallery

• 

  WB analysis of human heart using 10234-2-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10234-2-AP (Transgelin 2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10234-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10234-2-AP (Transgelin 2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 10234-2-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 10234-2-AP (Transgelin 2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10234-2-AP (Transgelin 2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10234-2-AP (Transgelin 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10234-2-AP (Transgelin 2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human urothelial carcinoma using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10234-2-AP (Transgelin 2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10234-2-AP (Transgelin 2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10234-2-AP (Transgelin 2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human urothelial carcinoma using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10234-2-AP (Transgelin 2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 10234-2-AP

  Immunofluorescent analysis of HepG2 cells using 10234-2-AP (Transgelin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human heart tissue, HEK-293 cells, T-47D cells
• Positive IHC detected in: human colon cancer tissue, human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 7 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below

Product Information

10234-2-AP targets Transgelin 2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Transgelin 2 fusion protein Ag0337
• Full Name: transgelin 2
• Calculated molecular weight: 199 aa, 22 kDa
• Observed molecular weight: 22 kDa
• GenBank accession number: BC002616
• Gene symbol: TAGLN2
• Gene ID (NCBI): 8407
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full length transgenlin 2 protein. It may cross react with other two transgelins based on the sequence similarity.

Publications

Species	Application	Title
human	ELISA	Mol Cell Proteomics; Comparative Tissue Proteomics of Microdissected Specimens Reveals Novel Candidate Biomarkers of Bladder Cancer.; Authors - Chien-Lun Chen
human	WB,IHC	J Proteome Res; Identification of vascular breast tumor markers by laser capture microdissection and label-free LC-MS.; Authors - Hill Jennifer J JJ
human	WB,IHC	J Proteome Res; Tissue proteomics reveals differential and compartment-specific expression of the homologs transgelin and transgelin-2 in lung adenocarcinoma and its stroma.; Authors - Rho Jung-Hyun JH
human	IHC	J Proteome Res; Post-translational modifications and protein-specific isoforms in endometriosis revealed by 2D DIGE.; Authors - Stephens Andrew N AN
human	WB	Front Oncol; Prognosis Analysis and Validation of m6A Signature and Tumor Immune Microenvironment in Glioma.; Authors - Shaojian Lin
human	WB,IHC,IF	Cancer Sci; Hypoxia-inducible transgelin 2 selects epithelial-to-mesenchymal transition and γ-radiation-resistant subtypes by focal adhesion kinase-associated insulin-like growth factor 1 receptor activation in non-small-cell lung cancer cells.; Authors - In-Gyu Kim ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joleen (Verified Customer) (06-16-2019)

It recognizes transgelin (prominent lower band) and some other bands, albeit much more weakly.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

Transgelin 2 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10234-2-AP)

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