Transgelin 2 Polyclonal antibody

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  WB analysis using 10234-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10234-2-AP (Transgelin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10234-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10234-2-AP (Transgelin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 10234-2-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 10234-2-AP (Transgelin 2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10234-2-AP (Transgelin 2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human intrahepatic cholangiocarcinoma using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10234-2-AP (Transgelin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10234-2-AP (Transgelin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10234-2-AP (Transgelin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10234-2-AP (Transgelin 2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10234-2-AP (Transgelin 2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10234-2-AP (Transgelin 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10234-2-AP (Transgelin 2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human urothelial carcinoma using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10234-2-AP (Transgelin 2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human urothelial carcinoma using 10234-2-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10234-2-AP (Transgelin 2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 10234-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Transgelin 2 antibody (10234-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HepG2 using 10234-2-AP

  Immunofluorescent analysis of HepG2 cells using 10234-2-AP (Transgelin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

Transgelin 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10234-2-AP

Publications(22)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

TAGLN2, Transgelin-2, Transgelin 2/TAGLN2, SM22-alpha homolog, Epididymis tissue protein Li 7e

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells
• Positive IHC detected in: human skin cancer tissue, human colon cancer tissue, human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue, human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 17 publications below
• IHC: See 9 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below

Product Information

10234-2-AP targets Transgelin 2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Transgelin 2 fusion protein Ag0337
• Full Name: transgelin 2
• Calculated Molecular Weight: 199 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC002616
• Gene Symbol: Transgelin-2/TAGLN2
• Gene ID (NCBI): 8407
• RRID: AB_2240241
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P37802
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full length transgenlin 2 protein. It may cross react with other two transgelins based on the sequence similarity.

Publications

Species	Application	Title
human	ELISA	Mol Cell Proteomics; Comparative Tissue Proteomics of Microdissected Specimens Reveals Novel Candidate Biomarkers of Bladder Cancer.; Authors - Chien-Lun Chen
human	WB,IHC	J Proteome Res; Identification of vascular breast tumor markers by laser capture microdissection and label-free LC-MS.; Authors - Hill Jennifer J JJ
human	WB,IHC	J Proteome Res; Tissue proteomics reveals differential and compartment-specific expression of the homologs transgelin and transgelin-2 in lung adenocarcinoma and its stroma.; Authors - Rho Jung-Hyun JH
human	IHC	J Proteome Res; Post-translational modifications and protein-specific isoforms in endometriosis revealed by 2D DIGE.; Authors - Stephens Andrew N AN
human	WB	Front Oncol; Prognosis Analysis and Validation of m6A Signature and Tumor Immune Microenvironment in Glioma.; Authors - Shaojian Lin
human	WB,IHC,IF	Cancer Sci; Hypoxia-inducible transgelin 2 selects epithelial-to-mesenchymal transition and γ-radiation-resistant subtypes by focal adhesion kinase-associated insulin-like growth factor 1 receptor activation in non-small-cell lung cancer cells.; Authors - In-Gyu Kim ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (06-16-2019)

It recognizes transgelin (prominent lower band) and some other bands, albeit much more weakly.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

Transgelin 2 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10234-2-AP)