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  • KD/KO Validated

Transgelin 2 Polyclonal antibody

Transgelin 2 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10234-2-AP

Synonyms

SM22 beta, SM22beta, TAGLN2, Transgelin 2



Tested Applications

Positive WB detected inhuman heart tissue, HEK-293 cells, T-47D cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10234-2-AP targets Transgelin 2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Transgelin 2 fusion protein Ag0337
Full Name transgelin 2
Calculated molecular weight 199 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC002616
Gene symbol TAGLN2
Gene ID (NCBI) 8407
RRIDAB_2240241
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full length transgenlin 2 protein. It may cross react with other two transgelins based on the sequence similarity.

Protocols

Product Specific Protocols
WB protocol for Transgelin 2 antibody 10234-2-APDownload protocol
IHC protocol for Transgelin 2 antibody 10234-2-APDownload protocol
IF protocol for Transgelin 2 antibody 10234-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanELISA

Mol Cell Proteomics

Comparative Tissue Proteomics of Microdissected Specimens Reveals Novel Candidate Biomarkers of Bladder Cancer.

Authors - Chien-Lun Chen
humanWB,IHC

J Proteome Res

Identification of vascular breast tumor markers by laser capture microdissection and label-free LC-MS.

Authors - Hill Jennifer J JJ
humanWB,IHC

J Proteome Res

Tissue proteomics reveals differential and compartment-specific expression of the homologs transgelin and transgelin-2 in lung adenocarcinoma and its stroma.

Authors - Rho Jung-Hyun JH
humanIHC

J Proteome Res

Post-translational modifications and protein-specific isoforms in endometriosis revealed by 2D DIGE.

Authors - Stephens Andrew N AN
humanWB

Front Oncol

Prognosis Analysis and Validation of m6A Signature and Tumor Immune Microenvironment in Glioma.

Authors - Shaojian Lin
humanWB,IHC,IF

Cancer Sci

Hypoxia-inducible transgelin 2 selects epithelial-to-mesenchymal transition and γ-radiation-resistant subtypes by focal adhesion kinase-associated insulin-like growth factor 1 receptor activation in non-small-cell lung cancer cells.

Authors - In-Gyu Kim
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joleen (Verified Customer) (06-16-2019)

It recognizes transgelin (prominent lower band) and some other bands, albeit much more weakly.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
Transgelin 2 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10234-2-AP)