|Positive WB detected in||HepG2 cells, HeLa cells, mouse skeletal muscle tissue|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human pancreas cancer tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11114-1-AP targets TAP1 in WB, RIP, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TAP1 fusion protein Ag1619|
|Full Name||transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)|
|Calculated molecular weight||81 kDa|
|Observed molecular weight||70-81 kDa|
|GenBank accession number||BC014081|
|Gene ID (NCBI)||6890|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70-80 kDa in HeLa cells.
|Product Specific Protocols|
|WB protocol for TAP1 antibody 11114-1-AP||Download protocol|
|IHC protocol for TAP1 antibody 11114-1-AP||Download protocol|
|IF protocol for TAP1 antibody 11114-1-AP||Download protocol|
|IP protocol for TAP1 antibody 11114-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Loss of heterozygosity at 6p21 and HLA class I expression in esophageal squamous cell carcinomas in China.
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Methylation of the promoter of human leukocyte antigen class I in human esophageal squamous cell carcinoma and its histopathological characteristics.