• Featured Product
  • KD/KO Validated

TAP1 Polyclonal antibody

TAP1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, RIP, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11114-1-AP

Synonyms

ABCB2, Antigen peptide transporter 1, PSF 1, RING 4, RING4, TAP1



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, mouse skeletal muscle tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11114-1-AP targets TAP1 in WB, RIP, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TAP1 fusion protein Ag1619
Full Name transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
Calculated molecular weight 81 kDa
Observed molecular weight 70-81 kDa
GenBank accession numberBC014081
Gene symbol TAP1
Gene ID (NCBI) 6890
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70-80 kDa in HeLa cells.

Protocols

Product Specific Protocols
WB protocol for TAP1 antibody 11114-1-APDownload protocol
IHC protocol for TAP1 antibody 11114-1-APDownload protocol
IF protocol for TAP1 antibody 11114-1-APDownload protocol
IP protocol for TAP1 antibody 11114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Asian Pac J Cancer Prev

Loss of heterozygosity at 6p21 and HLA class I expression in esophageal squamous cell carcinomas in China.

Authors - Zhao Xiaogang X
humanIHC

Photodiagnosis Photodyn Ther

Expression of coproporphyrinogen oxidase is associated with detection of upper gastrointestinal carcinomas by 5-aminolevulinic acid-mediated photodynamic diagnosis.

Authors - Kumi Ogihara
humanWB

Int Immunopharmacol

IRG1 increases MHC class I level in macrophages through STAT-TAP1 axis depending on NADPH oxidase mediated reactive oxygen species.

Authors - Xing Liu
humanIHC

Hum Pathol

Down-regulation of the antigen processing machinery is linked to a loss of inflammatory response in colorectal cancer.

Authors - Kasajima Atsuko A
mouseWB,RIP

Cancer Med

Noncoding RNA 886 alleviates tumor cellular immunological rejection in host C57BL/C mice.

Authors - Hui Ma
humanIHC

J Thorac Cardiovasc Surg

Methylation of the promoter of human leukocyte antigen class I in human esophageal squamous cell carcinoma and its histopathological characteristics.

Authors - Qifeng Sun S