Tested Applications
| Positive WB detected in | HeLa cells, Y79 cells, pig brain tissue, SH-SY5Y cells, U-251 cells, Neuro-2a cells, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human gliomas tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:30000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66499-1-Ig targets TAU in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Mouse / IgG2c |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21926 Product name: Recombinant human TAU protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 5-208 aa of BC000558 Sequence: PRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENL Predict reactive species |
| Full Name | microtubule-associated protein tau |
| Calculated Molecular Weight | 37-46, 79-81 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC000558 |
| Gene Symbol | TAU |
| Gene ID (NCBI) | 4137 |
| RRID | AB_2881863 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P10636 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The microtubule-associated protein TAU (MAPT or TAU) is encoded by MAPT gene, which locates on human chromosome 17q21, binds to the tubulin subunit of microtubule and promotes its assembly and stability. Most TAU is expressed in neurons, and TAU isoform is expressed in the peripheral nervous system while the others are expressed in the central nervous system. TAU links axonal microtubules with C-terminus to neural plasma membrane components with its N-terminus, suggesting the participation in intracellular signal transduction and neuron's development and viability. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (PMID:8752131,15965697, 12485403).Present monoclonal anti-Tau antibody can detect approx 100-kDa bands in brain tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TAU antibody 66499-1-Ig | Download protocol |
| IHC protocol for TAU antibody 66499-1-Ig | Download protocol |
| WB protocol for TAU antibody 66499-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
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| Species | Application | Title |
|---|---|---|
Adv Mater Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment | ||
Acta Pharm Sin B Pleiotropic prodrugs for both symptomatic and disease-modifying treatment of Alzheimer's disease. | ||
Adv Sci (Weinh) Advanced Stem Cell Therapy: 3D-Bioprinted Brain-Like Transplants for Alzheimer's Disease-Like Dementia. | ||
Adv Sci (Weinh) Tau Aggregation-Dependent Lipid Peroxide Accumulation Driven by the hsa_circ_0001546/14-3-3/CAMK2D/Tau Complex Inhibits Epithelial Ovarian Cancer Peritoneal Metastasis | ||
Phytomedicine Taohe Chengqi decoction improves diabetic cognitive dysfunction by alleviating neural stem cell senescence through HIF1α-driven metabolic signaling |
Reviews
What customers say
Customers report positive experiences with this antibody for Western Blot. One user found it effective for detecting recombinant tau at a 1:5000 dilution. Another praised it for producing a clear, specific signal in SH-SY5Y cells at 1:1000 dilution, and also appreciated the prompt shipping and careful cold-chain handling. Overall, reviewers rate the product favorably, highlighting its specificity and reliable performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Virender (Verified Customer) (01-09-2020) | Tau antibody works well for recombinant tau as well.
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Mary (Verified Customer) (11-08-2019) | I like this antibody. It provides a clear specific signal. The shipping was prompt and the antibody was kept on ice the whole time which I appreciated.
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