|Positive WB detected in||HeLa cells, SH-SY5Y cells, U-251 cells, Y79 cells, pig brain tissue|
|Positive IHC detected in||human gliomas tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:30000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66499-1-Ig targets TAU in WB, IHC,ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
|Tested Reactivity||Human, mouse, rat, pig|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2c|
|Immunogen||TAU fusion protein Ag21926|
|Full Name||microtubule-associated protein tau|
|Calculated molecular weight||37-46, 79-81 kDa|
|Observed molecular weight||100 kDa|
|GenBank accession number||BC000558|
|Gene ID (NCBI)||4137|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The microtubule-associated protein TAU (MAPT or TAU) is encoded by MAPT gene, which locates on human chromosome 17q21, binds to the tubulin subunit of microtubule and promotes its assembly and stability. Most TAU is expressed in neurons, and TAU isoform is expressed in the peripheral nervous system while the others are expressed in the central nervous system. TAU links axonal microtubules with C-terminus to neural plasma membrane components with its N-terminus, suggesting the participation in intracellular signal transduction and neuron's development and viability.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Virender (Verified Customer) (01-09-2020)
Tau antibody works well for recombinant tau as well.
Mary (Verified Customer) (11-08-2019)
I like this antibody. It provides a clear specific signal. The shipping was prompt and the antibody was kept on ice the whole time which I appreciated.