TAU Monoclonal antibody

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• KD/KO Validated

TAU Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

10

CloneNo.

1E9A8

Reviews (2)

Cat no : 66499-1-Ig

Synonyms

DDPAC, FTDP 17, MAPT, MAPTL, MSTD, MTBT1, MTBT2, Neurofibrillary tangle protein, Paired helical filament tau, PHF tau, PPND, TAU

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Validation Data Gallery

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  WB analysis using 66499-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66499-1-Ig (TAU antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66499-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66499-1-Ig (TAU antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66499-1-Ig

  WB result of TAU antibody (66499-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAU transfected HeLa cells.

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  WB analysis of SH-SY5Y using 66499-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66499-1-Ig (TAU antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 66499-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 66499-1-Ig (TAU antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 66499-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 66499-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66499-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66499-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, SH-SY5Y cells, U-251 cells, Y79 cells, pig brain tissue
• Positive IHC detected in: human gliomas tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:30000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 9 publications below
• IF: See 1 publications below

Product Information

66499-1-Ig targets TAU in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2c
• Class: Monoclonal
• Type: Antibody
• Immunogen: TAU fusion protein Ag21926
• Full Name: microtubule-associated protein tau
• Calculated molecular weight: 37-46, 79-81 kDa
• Observed molecular weight: 100 kDa
• GenBank accession number: BC000558
• Gene symbol: MAPT
• Gene ID (NCBI): 4137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The microtubule-associated protein TAU (MAPT or TAU) is encoded by MAPT gene, which locates on human chromosome 17q21, binds to the tubulin subunit of microtubule and promotes its assembly and stability. Most TAU is expressed in neurons, and TAU isoform is expressed in the peripheral nervous system while the others are expressed in the central nervous system. TAU links axonal microtubules with C-terminus to neural plasma membrane components with its N-terminus, suggesting the participation in intracellular signal transduction and neuron's development and viability.

Publications

Species	Application	Title
mouse	WB	Front Aging Neurosci; Salidroside Ameliorates Alzheimer's Disease by Targeting NLRP3 Inflammasome-Mediated Pyroptosis.; Authors - Yawen Cai
mouse	WB	Mol Med; Eriodictyol ameliorates cognitive dysfunction in APP/PS1 mice by inhibiting ferroptosis via vitamin D receptor-mediated Nrf2 activation.; Authors - Lin Li
mouse	WB	Environ Sci Pollut Res Int; Acute lead acetate induces neurotoxicity through decreased synaptic plasticity-related protein expression and disordered dendritic formation in nerve cells.; Authors - Lingli Chen
mouse	WB	Comput Math Methods Med; Acupuncture Effect Assessment in APP/PS1 Transgenic Mice: On Regulating Learning-Memory Abilities, Gut Microbiota, and Microbial Metabolites.; Authors - Bo Yang
human	WB	Cell Rep; The balance of mitochondrial fission and fusion in cortical axons depends on the kinases SadA and SadB.; Authors - Danila Di Meo
mouse	WB	Acta Pharmacol Sin; Long-term running exercise alleviates cognitive dysfunction in APP/PSEN1 transgenic mice via enhancing brain lysosomal function.; Authors - Xue Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Virender (Verified Customer) (01-09-2020)

Tau antibody works well for recombinant tau as well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000

FH

Mary (Verified Customer) (11-08-2019)

I like this antibody. It provides a clear specific signal. The shipping was prompt and the antibody was kept on ice the whole time which I appreciated.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SH-SY5Y