TBCB Polyclonal antibody

TBCB Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15782-1-AP

Synonyms

CG22, CKAP1, CKAPI, TBCB, tubulin folding cofactor B, Tubulin specific chaperone B



Tested Applications

Positive WB detected inA431 cells, HeLa cells, NCI-H1299 cells, Neuro-2a cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

15782-1-AP targets TBCB in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBCB fusion protein Ag8473
Full Name tubulin folding cofactor B
Calculated Molecular Weight 244 aa, 27 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC005969
Gene Symbol TBCB
Gene ID (NCBI) 1155
RRIDAB_2302986
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TBCB antibody 15782-1-APDownload protocol
IF protocol for TBCB antibody 15782-1-APDownload protocol
IP protocol for TBCB antibody 15782-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Histochem Cell Biol

Spatio-temporal distribution of tubulin-binding cofactors and posttranslational modifications of tubulin in the cochlea of mice.

Authors - Lukas Juergens