TCTN1 Polyclonal antibody

TCTN1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15004-1-AP

Synonyms

TCTN1, TECT1, Tectonic 1, tectonic family member 1



Tested Applications

Positive WB detected infetal human brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman kidney tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 15004-1-AP is TCTN1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCTN1 fusion protein Ag6099
Full Name tectonic family member 1
Calculated molecular weight 64 kDa
Observed molecular weight 55-64 kDa
GenBank accession numberBC062611
Gene symbol TCTN1
Gene ID (NCBI) 79600
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tectonic-1, encoded by TCTN1 (TECT1) gene, belongs to the tectonic family of secreted and transmembrane proteins. Tectonic-1 is a component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Multiple transcript variants encoding different isoforms have been found for this gene. Defects in TCTN1 are the cause of Joubert syndrome type 13 (JBTS13), a disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay.During neural tube development, mouse Tectonic is required for formation of the most ventral cell types and for full Hedgehog (Hh) pathway activation. Epistasis analyses reveal that Tectonic modulates Hh signal transduction downstream of Smoothened (Smo) and Rab23.

Protocols

Product Specific Protocols
WB protocol for TCTN1 antibody 15004-1-APDownload protocol
IHC protocol for TCTN1 antibody 15004-1-APDownload protocol
IF protocol for TCTN1 antibody 15004-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Curr Biol

NUP98 Sets the Size-Exclusion Diffusion Limit through the Ciliary Base.

Authors - S Joseph Endicott
humanIF

Methods Cell Biol

Immunofluorescent staining of septins in primary cilia.

Authors - M S Kim
humanIF

Biol Open

Interaction of INPP5E with ARL13B is essential for its ciliary membrane retention but dispensable for its ciliary entry.

Authors - Hantian Qiu
humanIF

Dev Biol

A function for the Joubert syndrome protein Arl13b in ciliary membrane extension and ciliary length regulation.

Authors - Hao Lu
humanIF

J Cell Sci

Loss of OCRL increases ciliary PI(4,5)P2 in oculocerebrorenal syndrome of Lowe.

Authors - Philipp P Prosseda
humanIF

FASEB J

ESCRT subunit CHMP4B localizes to primary cilia and is required for the structural integrity of the ciliary membrane.

Authors - Eunji Jung
  • KD Validated