|Positive WB detected in||fetal human brain tissue, mouse brain tissue, rat brain tissue|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 15004-1-AP is TCTN1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TCTN1 fusion protein Ag6099|
|Full Name||tectonic family member 1|
|Calculated molecular weight||64 kDa|
|Observed molecular weight||55-64 kDa|
|GenBank accession number||BC062611|
|Gene ID (NCBI)||79600|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Tectonic-1, encoded by TCTN1 (TECT1) gene, belongs to the tectonic family of secreted and transmembrane proteins. Tectonic-1 is a component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Multiple transcript variants encoding different isoforms have been found for this gene. Defects in TCTN1 are the cause of Joubert syndrome type 13 (JBTS13), a disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay.During neural tube development, mouse Tectonic is required for formation of the most ventral cell types and for full Hedgehog (Hh) pathway activation. Epistasis analyses reveal that Tectonic modulates Hh signal transduction downstream of Smoothened (Smo) and Rab23.
NUP98 Sets the Size-Exclusion Diffusion Limit through the Ciliary Base.
Methods Cell Biol
Immunofluorescent staining of septins in primary cilia.
A function for the Joubert syndrome protein Arl13b in ciliary membrane extension and ciliary length regulation.
J Cell Sci
Loss of OCRL increases ciliary PI(4,5)P2 in oculocerebrorenal syndrome of Lowe.
ESCRT subunit CHMP4B localizes to primary cilia and is required for the structural integrity of the ciliary membrane.
A compartmentalized phosphoinositide signaling axis at cilia is regulated by INPP5E to maintain cilia and promote Sonic Hedgehog medulloblastoma.