• Featured Product
  • KD/KO Validated

TCTN2 Polyclonal antibody

TCTN2 Polyclonal Antibody for IF, IHC, IP,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17053-1-AP

Synonyms

C12orf38, TCTN2, TECT2, Tectonic 2, tectonic family member 2



Tested Applications

Positive IP detected inRPE1 cells(siRNA)
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17053-1-AP targets TCTN2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCTN2 fusion protein Ag10725
Full Name tectonic family member 2
Calculated molecular weight 697 aa, 77 kDa
GenBank accession numberBC009112
Gene symbol TCTN2
Gene ID (NCBI) 79867
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TCTN2 (tectonic-2 or TECT2) is a type I membrane protein that belongs to the tectonic family, which consists of TCTN1, TCTN2 and TCTN3. Studies in mice suggest that tectonic may be involved in Hedgehog (Hh) signaling, and essential for ciliogenesis. Tectonic is component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. It exists two spilcing isoforms. The first aberrant transcript introduces 104 base pair from intron 13 and would delete 196 original amino acid, introduce two novel amino acids, and prematurely truncate the 697aa protein at residue number 504. The second transcript lacks exon 14 and would delete 195 original amino acids, introduce four novel amino acids, and prematurely truncate the 697 aa protein at residue number 507. The molecular weight of two isoforms are about 50kDa. We detected the 50kDa and 90kDa protein by immunoprecipitaion, but 50kDa protein may be the splicing isoform or non-specific band.

Protocols

Product Specific Protocols
IHC protocol for TCTN2 antibody 17053-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Cell Biol

Phosphorylation of CEP83 by TTBK2 is necessary for cilia initiation.

Authors - Chien-Hui Lo
mouseIF

EMBO J

Cell type-specific regulation of ciliary transition zone assembly in vertebrates.

Authors - Antonia Wiegering
human

Nat Commun

Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.

Authors - Hai-Qing Tu
humanWB,IF

Nat Cell Biol

CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base.

Authors - Wang Won-Jing WJ
mouseWB

Cell

Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathways.

Authors - Sang Liyun L
humanWB,IF

Nat Genet

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Authors - Garcia-Gonzalo Francesc R FR

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jay (Verified Customer) (12-02-2019)

Methanol Fixation.Immunostaining result shows that Tctn2 localizes to centriole in ciliated NIH3T3 cell (Tctn2;red, Arl13b;green for primary cilia, DAPI;blue for nucleus).The antibody seems to have noisy background in cytoplasm.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse NIH3T3
TCTN2 Antibody Immunofluorescence, validation (1:100 dilution) in Mouse NIH3T3 (Cat no:17053-1-AP)