|Positive IP detected in||RPE1 cells(siRNA)|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||hTERT-RPE1 cells|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
17053-1-AP targets TCTN2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TCTN2 fusion protein Ag10725|
|Full Name||tectonic family member 2|
|Calculated molecular weight||697 aa, 77 kDa|
|GenBank accession number||BC009112|
|Gene ID (NCBI)||79867|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TCTN2 (tectonic-2 or TECT2) is a type I membrane protein that belongs to the tectonic family, which consists of TCTN1, TCTN2 and TCTN3. Studies in mice suggest that tectonic may be involved in Hedgehog (Hh) signaling, and essential for ciliogenesis. Tectonic is component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. It exists two spilcing isoforms. The first aberrant transcript introduces 104 base pair from intron 13 and would delete 196 original amino acid, introduce two novel amino acids, and prematurely truncate the 697aa protein at residue number 504. The second transcript lacks exon 14 and would delete 195 original amino acids, introduce four novel amino acids, and prematurely truncate the 697 aa protein at residue number 507. The molecular weight of two isoforms are about 50kDa. We detected the 50kDa and 90kDa protein by immunoprecipitaion, but 50kDa protein may be the splicing isoform or non-specific band.
J Cell Biol
Phosphorylation of CEP83 by TTBK2 is necessary for cilia initiation.
Cell type-specific regulation of ciliary transition zone assembly in vertebrates.
Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.
Nat Cell Biol
CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base.
Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathways.
A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jay (Verified Customer) (12-02-2019)
Methanol Fixation.Immunostaining result shows that Tctn2 localizes to centriole in ciliated NIH3T3 cell (Tctn2;red, Arl13b;green for primary cilia, DAPI;blue for nucleus).The antibody seems to have noisy background in cytoplasm.