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  • KD/KO Validated

TDG Polyclonal antibody

TDG Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ChIP,ELISA

Conjugate

Unconjugated

Cat no : 13370-1-AP

Synonyms

TDG, thymine DNA glycosylase



Tested Applications

Positive WB detected inhuman brain tissue, HeLa cells, mouse colon tissue, mouse thymus tissue, Raji cells
Positive IP detected inU-937 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13370-1-AP targets TDG in WB, IP, IHC, IF, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TDG fusion protein Ag4190
Full Name thymine-DNA glycosylase
Calculated molecular weight 410 aa, 46 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC037557
Gene symbol TDG
Gene ID (NCBI) 6996
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TDG belongs to the TDG/mug DNA glycosylase family. TDG corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. RNF4 interacts with and requires the base excision repair enzymes TDG and APE1 for active demethylation (PMID:20696907). TDG is modified by SUMO-1 and SUMO-2/3.The molecular weight of non-modified TDG is 46 kDa and modified TDG is 55-60 kDa.

Protocols

Product Specific Protocols
WB protocol for TDG antibody 13370-1-APDownload protocol
IF protocol for TDG antibody 13370-1-APDownload protocol
IP protocol for TDG antibody 13370-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

The HOTAIRM1/miR-107/TDG axis regulates papillary thyroid cancer cell proliferation and invasion.

Authors - Dan Li
humanIHC,ChIP

J Biol Chem

Thymine DNA Glycosylase is a Positive Regulator of Wnt Signaling in Colorectal Cancer.

Authors - Xuehe Xu
humanWB

J Biol Chem

CRL4Cdt2 E3 Ubiquitin Ligase and PCNA Cooperate to Degrade Thymine DNA Glycosylase in S-phase.

Authors - Etsuko Shibata
humanWB

Proc Natl Acad Sci U S A

Identification of RING finger protein 4 (RNF4) as a modulator of DNA demethylation through a functional genomics screen.

Authors - Hu Xiaoyi V XV
  • KD Validated
humanWB,IP

J Cell Biol

Oxidative stress-induced assembly of PML nuclear bodies controls sumoylation of partner proteins.

Authors - Umut Sahin