|Positive WB detected in||human brain tissue, HeLa cells, mouse colon tissue, mouse thymus tissue, Raji cells|
|Positive IP detected in||U-937 cells|
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13370-1-AP targets TDG in WB, IP, IHC, IF, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TDG fusion protein Ag4190|
|Full Name||thymine-DNA glycosylase|
|Calculated molecular weight||410 aa, 46 kDa|
|Observed molecular weight||55-60 kDa|
|GenBank accession number||BC037557|
|Gene ID (NCBI)||6996|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TDG belongs to the TDG/mug DNA glycosylase family. TDG corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. RNF4 interacts with and requires the base excision repair enzymes TDG and APE1 for active demethylation (PMID:20696907). TDG is modified by SUMO-1 and SUMO-2/3.The molecular weight of non-modified TDG is 46 kDa and modified TDG is 55-60 kDa.
Cell Death Dis
The HOTAIRM1/miR-107/TDG axis regulates papillary thyroid cancer cell proliferation and invasion.
J Biol Chem
Thymine DNA Glycosylase is a Positive Regulator of Wnt Signaling in Colorectal Cancer.
J Biol Chem
CRL4Cdt2 E3 Ubiquitin Ligase and PCNA Cooperate to Degrade Thymine DNA Glycosylase in S-phase.
Proc Natl Acad Sci U S A
Identification of RING finger protein 4 (RNF4) as a modulator of DNA demethylation through a functional genomics screen.
J Cell Biol
Oxidative stress-induced assembly of PML nuclear bodies controls sumoylation of partner proteins.