|Positive WB detected in
|HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells
|Positive IP detected in
|Positive IHC detected in
|human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:500-1:2000
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
22586-1-AP targets TFAM in WB, IP, IHC, IF, chIP, ELISA applications and shows reactivity with human samples.
|human, chicken, zebrafish
|Host / Isotype
|Rabbit / IgG
|TFAM fusion protein Ag18299
|transcription factor A, mitochondrial
|Calculated molecular weight
|246 aa, 29 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.
|Product Specific Protocols
|WB protocol for TFAM antibody 22586-1-AP
|IHC protocol for TFAM antibody 22586-1-AP
|IF protocol for TFAM antibody 22586-1-AP
|IP protocol for TFAM antibody 22586-1-AP
|Click here to view our Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sebastian (Verified Customer) (10-25-2023)
Good detection signals for TFAM in western blot of liver from old and young mice, some unspecific bands were slightly visible
Tanusree (Verified Customer) (12-18-2019)
Product worked well in WB at 1:500 dilution.