- Featured Product
- KD/KO Validated
TFAM Polyclonal antibody
TFAM Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human and More (2)
WB, IP, IHC, IF, chIP, ELISA
Cat no : 22586-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, human testis tissue, A431 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 3 publications below|
|WB||See 70 publications below|
|IHC||See 9 publications below|
|IF||See 3 publications below|
|IP||See 1 publications below|
|ChIP||See 1 publications below|
22586-1-AP targets TFAM in WB, IP, IHC, IF, chIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, rat, chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TFAM fusion protein Ag18299|
|Full Name||transcription factor A, mitochondrial|
|Calculated molecular weight||246 aa, 29 kDa|
|Observed molecular weight||25 kDa|
|GenBank accession number||BC126366|
|Gene ID (NCBI)||7019|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.
|Product Specific Protocols|
|WB protocol for TFAM antibody 22586-1-AP||Download protocol|
|IHC protocol for TFAM antibody 22586-1-AP||Download protocol|
|IF protocol for TFAM antibody 22586-1-AP||Download protocol|
|IP protocol for TFAM antibody 22586-1-AP||Download protocol|
|Click here to view our Standard Protocols|
A plant-derived natural photosynthetic system for improving cell anabolism
Extracellular Tuning of Mitochondrial Respiration Leads to Aortic Aneurysm.
Mitochondrial Damage and Activation of the STING Pathway Lead to Renal Inflammation and Fibrosis.
Nat Cell Biol
Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.
Mater Today Bio
Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction
Cell Death Differ
Inhibition of cGAS-STING by JQ1 alleviates oxidative stress-induced retina inflammation and degeneration.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tanusree (Verified Customer) (12-18-2019)
Product worked well in WB at 1:500 dilution.