TFAM Polyclonal antibody

• Featured Product
• KD/KO Validated

TFAM Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Publications

76

Reviews (1)

Cat no : 22586-1-AP

Synonyms

Cell and organelle markers, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, MtTF1, mtTFA, TCF 6, TCF6, TCF6L1, TCF6L2, TCF6L3, TFAM, Transcription factor 6, Transcription factor 6 like 2

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Validation Data Gallery

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  WB analysis using 22586-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22586-1-AP (TFAM antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 22586-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 22586-1-AP (TFAM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 22586-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22586-1-AP (TFAM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 22586-1-AP

  IP Result of anti-TFAM (IP:22586-1-AP, 4ug; Detection:22586-1-AP 1:800) with HEK-293 cells lysate 3200ug.

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  IHC staining of human colon cancer using 22586-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22586-1-AP (TFAM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22586-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22586-1-AP (TFAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 22586-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (22586-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human testis tissue, A431 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 70 publications below
• IHC: See 9 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Product Information

22586-1-AP targets TFAM in WB, IP, IHC, IF, chIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TFAM fusion protein Ag18299
• Full Name: transcription factor A, mitochondrial
• Calculated molecular weight: 246 aa, 29 kDa
• Observed molecular weight: 25 kDa
• GenBank accession number: BC126366
• Gene symbol: TFAM
• Gene ID (NCBI): 7019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.

Publications

Species	Application	Title
mouse	WB	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
mouse	WB，IHC	Circulation; Extracellular Tuning of Mitochondrial Respiration Leads to Aortic Aneurysm.; Authors - Jorge Oller
mouse	IHC	Cell Metab; Mitochondrial Damage and Activation of the STING Pathway Lead to Renal Inflammation and Fibrosis.; Authors - Ki Wung Chung
human	WB	Nat Cell Biol; Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.; Authors - Timo Rey
human,rat	WB	Mater Today Bio; Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction; Authors - Zhanqiu Dai
mouse	WB	Cell Death Differ; Inhibition of cGAS-STING by JQ1 alleviates oxidative stress-induced retina inflammation and degeneration.; Authors - Ming Zou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain