TFAM Polyclonal antibody

TFAM Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Publications

42

Reviews (1)

Cat no : 22586-1-AP

Synonyms

Cell and organelle markers, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, MtTF1, mtTFA, TCF 6, TCF6, TCF6L1, TCF6L2, TCF6L3, TFAM, Transcription factor 6, Transcription factor 6 like 2

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Validation Data Gallery

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  WB analysis of MCF-7 using 22586-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22586-1-AP (TFAM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 22586-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 22586-1-AP (TFAM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 22586-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22586-1-AP (TFAM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22586-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22586-1-AP (TFAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22586-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22586-1-AP (TFAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22586-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22586-1-AP (TFAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 22586-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22586-1-AP (TFAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 22586-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 22586-1-AP (TFAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 22586-1-AP

  IP Result of anti-TFAM (IP:22586-1-AP, 4ug; Detection:22586-1-AP 1:800) with HEK-293 cells lysate 3200ug.

• 

  IHC staining of human testis using 22586-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22586-1-AP (TFAM Antibody) at dilution of 1:50.

• 

  IHC staining of human colon cancer using 22586-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22586-1-AP (TFAM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22586-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22586-1-AP (TFAM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 22586-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22586-1-AP (TFAM Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 22586-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (22586-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HEK-293 cells, Jurkat cells, HeLa cells, HepG2 cells, human testis tissue, A431 cells, K-562 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 38 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Product Information

The immunogen of 22586-1-AP is TFAM Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TFAM fusion protein Ag18299
• Full Name: transcription factor A, mitochondrial
• Calculated molecular weight: 246 aa, 29 kDa
• Observed molecular weight: 25 kDa
• GenBank accession number: BC126366
• Gene symbol: TFAM
• Gene ID (NCBI): 7019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.

Publications

Species	Application	Title
mouse	WB	Oncotarget; Liraglutide suppresses obesity and induces brown fat-like phenotype via Soluble Guanylyl Cyclase mediated pathway in vivo and in vitro.; Authors - Endong Zhu
mouse,human	WB	ACS Nano; Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.; Authors - Meng Zhao ; KD Validated
rat	WB	BMC Complement Med Ther; Tanshinone IIA combined with CsA inhibit myocardial cell apoptosis induced by renal ischemia-reperfusion injury in obese rats.; Authors - He Tai
mouse	WB	J Clin Transl Hepatol; Icaritin Attenuates Lipid Accumulation by Increasing Energy Expenditure and Autophagy Regulated by Phosphorylating AMPK.; Authors - Yue Wu
human	WB,IF	Curr Protoc; Assessing Mitochondrial DNA Release into the Cytosol and Subsequent Activation of Innate Immune-related Pathways in Mammalian Cells.; Authors - Joshua D Bryant ; KD Validated
rat	WB	Evid Based Complement Alternat Med; RNA-Seq Profiling to Investigate the Mechanism of Qishen Granules on Regulating Mitochondrial Energy Metabolism of Heart Failure in Rats.; Authors - Hao He

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain