Validation Data Gallery
|Positive WB detected in||MCF-7 cells, HEK-293 cells, Jurkat cells, HeLa cells, HepG2 cells, human testis tissue, A431 cells, K-562 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human colon cancer tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22586-1-AP is TFAM Fusion Protein expressed in E. coli.
|Cited Reactivity||human, rat, chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TFAM fusion protein Ag18299|
|Full Name||transcription factor A, mitochondrial|
|Calculated molecular weight||246 aa, 29 kDa|
|Observed molecular weight||25 kDa|
|GenBank accession number||BC126366|
|Gene ID (NCBI)||7019|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.
|Product Specific Protocols|
|WB protocol for TFAM antibody 22586-1-AP||Download protocol|
|IHC protocol for TFAM antibody 22586-1-AP||Download protocol|
|IF protocol for TFAM antibody 22586-1-AP||Download protocol|
|IP protocol for TFAM antibody 22586-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tanusree (Verified Customer) (12-18-2019)
Product worked well in WB at 1:500 dilution.