TFAM Polyclonal antibody

TFAM Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, chIP,ELISA

Conjugate

Unconjugated

Publications

15

Reviews (1)

Cat no : 22586-1-AP

Synonyms

MtTF1, mtTFA, TCF 6, TCF6, TCF6L1, TCF6L2, TCF6L3, TFAM, Transcription factor 6, Transcription factor 6 like 2

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Validation Data Gallery

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  IHC staining of mouse colon using 22586-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22586-1-AP (TFAM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  WB analysis of MCF-7 using 22586-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22586-1-AP (TFAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 22586-1-AP

  IP Result of anti-TFAM (IP:22586-1-AP, 4ug; Detection:22586-1-AP 1:800) with HEK-293 cells lysate 3200ug.

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Tested Applications

• Positive WB detected in: MCF7 cells, K-562 cells, HeLa cells, human testis tissue, HEK-293 cells, A431 cells, Jurkat cells, HepG2 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: mouse colon tissue, human colon cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 2 publications below
• ChIP: See 1 publications below

Product Information

The immunogen of 22586-1-AP is TFAM Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: chicken, human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TFAM fusion protein Ag18299
• Full Name: transcription factor A, mitochondrial
• Calculated molecular weight: 246 aa, 29 kDa
• Observed molecular weight: 25 kDa
• GenBank accession number: BC126366
• Gene symbol: TFAM
• Gene ID (NCBI): 7019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.

Publications

Species	Application	Title
mouse	WB	Oncotarget; Liraglutide suppresses obesity and induces brown fat-like phenotype via Soluble Guanylyl Cyclase mediated pathway in vivo and in vitro.; Authors - Endong Zhu
mouse,human	WB	ACS Nano; Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.; Authors - Meng Zhao ; KD Validated
rat	WB	BMC Complement Med Ther; Tanshinone IIA combined with CsA inhibit myocardial cell apoptosis induced by renal ischemia-reperfusion injury in obese rats.; Authors - He Tai
human	WB	Int J Neurosci; Vascular endothelial growth factor alleviates mitochondrial dysfunction and suppression of mitochondrial biogenesis in models of Alzheimer's disease.; Authors - Xiangtian Liu
mouse	WB	Biochem Biophys Res Commun; Fibroblast growth factor 19 alleviates palmitic acid-induced mitochondrial dysfunction and oxidative stress via the AMPK/PGC-1α pathway in skeletal muscle.; Authors - Ai Guo
human	WB	Biochem Biophys Res Commun; Mesenchymal stem cell-conditioned medium improved mitochondrial function and alleviated inflammation and apoptosis in non-alcoholic fatty liver disease by regulating SIRT1.; Authors - Mengmeng Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain