TFAM Polyclonal antibody

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  WB analysis using 22586-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22586-1-AP (TFAM antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22586-1-AP

  WB result of TFAM antibody (22586-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TFAM transfected HEK-293 cells.

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  IP experiment of HEK-293 using 22586-1-AP

  IP result of anti-TFAM (IP:22586-1-AP, 4ug; Detection:22586-1-AP 1:800) with HEK-293 cells lysate 3200ug.

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  IHC staining of human colon cancer using 22586-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22586-1-AP (TFAM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22586-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22586-1-AP (TFAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 22586-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (22586-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 22586-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (22586-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

TFAM Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 22586-1-AP

Publications(166)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, IP, chIP, ELISA

Synonyms

MtTF1, mtTFA, TCF6, TCF-6, Transcription factor 6-like 2

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 151 publications below
• IHC: See 12 publications below
• IF: See 20 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• ChIP: See 1 publications below

Product Information

22586-1-AP targets TFAM in WB, IHC, IF/ICC, IP, chIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, chicken, zebrafish, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TFAM fusion protein Ag18299
• Full Name: transcription factor A, mitochondrial
• Calculated Molecular Weight: 246 aa, 29 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC126366
• Gene Symbol: TFAM
• Gene ID (NCBI): 7019
• RRID: AB_11182588
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q00059
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.

Publications

Species	Application	Title
	WB	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
	IHC	Adv Mater; Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine; Authors - Peng Lou
	WB，IHC	Circulation; Extracellular Tuning of Mitochondrial Respiration Leads to Aortic Aneurysm.; Authors - Jorge Oller
	IHC	Cell Metab; Mitochondrial Damage and Activation of the STING Pathway Lead to Renal Inflammation and Fibrosis.; Authors - Ki Wung Chung
human	WB	Nat Cell Biol; Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.; Authors - Timo Rey

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sebastian (Verified Customer) (10-25-2023)

Good detection signals for TFAM in western blot of liver from old and young mice, some unspecific bands were slightly visible

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: liver

FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain