TFAM Polyclonal antibody

TFAM Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 23996-1-AP

Synonyms

MtTF1, mtTFA, TCF 6, TCF6, TCF6L1, TCF6L2, TCF6L3, TFAM, Transcription factor 6, Transcription factor 6 like 2



Tested Applications

Positive WB detected inA431 cells, HeLa cells, MCF-7 cells, Jurkat cells, HEK-293 cells, HL-60 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23996-1-AP is TFAM Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TFAM fusion protein Ag21196
Full Name transcription factor A, mitochondrial
Calculated molecular weight 246 aa, 29 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC126366
Gene symbol TFAM
Gene ID (NCBI) 7019
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.

Protocols

Product Specific Protocols
WB protocol for TFAM antibody 23996-1-APDownload protocol
IHC protocol for TFAM antibody 23996-1-APDownload protocol
IF protocol for TFAM antibody 23996-1-APDownload protocol
IP protocol for TFAM antibody 23996-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability.

Authors - Anaïs Aulas
humanWB,IF

Cell Death Dis

Oxidative stress-induced mitophagy is suppressed by the miR-106b-93-25 cluster in a protective manner.

Authors - Cheng Zhang
humanWB

Biochim Biophys Acta Mol Basis Dis

Icariin protects human nucleus pulposus cells from hydrogen peroxide-induced mitochondria-mediated apoptosis by activating nuclear factor erythroid 2-related factor 2.

Authors - Wenbin Hua