|Positive WB detected in||A431 cells, HL-60 cells, HeLa cells, MCF-7 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
23996-1-AP targets TFAM in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||TFAM fusion protein Ag21196|
|Full Name||transcription factor A, mitochondrial|
|Calculated molecular weight||246 aa, 29 kDa|
|Observed molecular weight||25 kDa|
|GenBank accession number||BC126366|
|Gene ID (NCBI)||7019|
|Purification Method||Antigen Affinity purified|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.
|Product Specific Protocols|
|WB protocol for TFAM antibody 23996-1-AP||Download protocol|
|IHC protocol for TFAM antibody 23996-1-AP||Download protocol|
|IF protocol for TFAM antibody 23996-1-AP||Download protocol|
|IP protocol for TFAM antibody 23996-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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