TGF Beta 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TGF Beta 1 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

670

Reviews (8)

Cat no : 21898-1-AP

Synonyms

TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, Tgfb 1, TGFB

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Validation Data Gallery

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  WB analysis using 21898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 21898-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF Beta 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF Beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF Beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 21898-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21898-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 21898-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of human stomach cancer using 21898-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach cancer tissue using TGF Beta 1 antibody (21898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 21898-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TGF Beta 1 antibody (21898-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse skin tissue, A549 cells, HeLa cells, K-562 cells, L-929 cells, mouse spleen tissue, rat skin tissue
• Positive IF-P detected in: human stomach cancer tissue
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 532 publications below
• IHC: See 151 publications below
• IF: See 71 publications below
• IP: See 1 publications below
• ELISA: See 2 publications below
• CoIP: See 1 publications below

Product Information

21898-1-AP targets TGF Beta 1 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit, canine, zebrafish, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TGF Beta 1 fusion protein Ag13591
• Full Name: transforming growth factor, beta 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC000125
• Gene Symbol: TGFB1
• Gene ID (NCBI): 7040
• RRID: AB_2811115
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1).

This antibody detects the pro-TGF beta 1 and the cleaved fragment Latency-associated peptide.

Publications

Species	Application	Title
	IHC	Adv Mater; Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine; Authors - Peng Lou
human	WB	ACS Nano; Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.; Authors - Meng Zhao
human	WB	Blood; C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse; Authors - Li-Xue Yang
	WB,IF	Acta Pharm Sin B; Fucoidan-functionalized activated platelet-hitchhiking micelles simultaneously track tumor cells and remodel the immunosuppressive microenvironment for efficient metastatic cancer treatment.; Authors - Rong Guo
human	WB	Dev Cell; Endothelial progenitor cells control remodeling of uterine spiral arteries for the establishment of utero-placental circulation; Authors - Bin Tan
human	WB	Redox Biol; Lactate-induced activation of tumor-associated fibroblasts and IL-8-mediated macrophage recruitment promote lung cancer progression; Authors - Xuyu Gu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Andrea (Verified Customer) (03-22-2024)

We had a good confocal signal and beautiful bands in our experimental group of high TGFb

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:50-1:500
• Cell Tissue Type: guinea pig myocardium

FH

Kenzo (Verified Customer) (08-15-2023)

This antibody works well for labeling TGF beta 1 on mouse kidney tissue section. Very reliable antibody giving reproducible results.

• Applications: Immunofluorescence
• Cell Tissue Type: Mouse Kidney

FH

Celina (Verified Customer) (07-31-2023)

antibody worked well for IF of human cardiac ventricular fibroblasts

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: human cardiac ventricular fibroblasts

FH

Udesh (Verified Customer) (12-06-2022)

The antibody worked well in Western Blot and detected bands for mature TGF-B1 as well as LAP.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

TGF Beta 1 Antibody Western Blot validation (1:1000 dilution) in (Cat no:21898-1-AP)

FH

Yasuyo (Verified Customer) (05-24-2022)

Works great!!!

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: lung

TGF Beta 1 Antibody Immunohistochemistry validation (1:250 dilution) in lung (Cat no:21898-1-AP)

FH

P (Verified Customer) (12-01-2021)

Excellent

• Applications: Western Blot
• Cell Tissue Type: fibroblast

TGF Beta 1 Antibody Western Blot validation ( dilution) in fibroblast (Cat no:21898-1-AP)

FH

Iram (Verified Customer) (09-04-2020)

Very clean bands for western

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 for western
• Cell Tissue Type: Human colon cancer

FH

Qingyuan (Verified Customer) (11-05-2019)

The antibody clearly shows one band with the right molecular weight size.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse spinal cord

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