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TGF Beta 1 Polyclonal antibody

TGF Beta 1 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (4)


WB, IP, IF, IHC, CoIP, ELISA, Cell treatment



Cat no : 21898-1-AP


CED, DPD1, LAP, Mouse Transforming Growth Factor β1, TGF beta 1, tgf beta1, TGFB, TGFB1, TGFbeta, TGF-beta 1, tgfbeta1, tgf-beta1, TGFβ, TGF-β, TGFβ1, TGF-β1

Tested Applications

Positive WB detected inmouse skin tissue, K-562 cells, HeLa cells, A549 cells, rat skin tissue
Positive IP detected inmouse skin tissue
Positive IF detected inHEK-293 cells, human stomach cancer tissue

Recommended dilution

Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21898-1-AP targets TGF Beta 1 in WB, IP, IF, IHC, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rabbit, zebrafish, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TGF Beta 1 fusion protein Ag13591
Full Name transforming growth factor, beta 1
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC000125
Gene Symbol TGFB1
Gene ID (NCBI) 7040
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1).

This antibody detects the pro-TGF beta 1 and the cleaved fragment Latency-associated peptide.


Product Specific Protocols
WB protocol for TGF Beta 1 antibody 21898-1-APDownload protocol
IF protocol for TGF Beta 1 antibody 21898-1-APDownload protocol
IP protocol for TGF Beta 1 antibody 21898-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Andrea (Verified Customer) (03-22-2024)

We had a good confocal signal and beautiful bands in our experimental group of high TGFb

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:50-1:500
  • Cell Tissue Type: guinea pig myocardium

Kenzo (Verified Customer) (08-15-2023)

This antibody works well for labeling TGF beta 1 on mouse kidney tissue section. Very reliable antibody giving reproducible results.

  • Applications: Immunofluorescence
  • Cell Tissue Type: Mouse Kidney

Celina (Verified Customer) (07-31-2023)

antibody worked well for IF of human cardiac ventricular fibroblasts

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: human cardiac ventricular fibroblasts

Udesh (Verified Customer) (12-06-2022)

The antibody worked well in Western Blot and detected bands for mature TGF-B1 as well as LAP.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
TGF Beta 1 Antibody Western Blot validation (1:1000 dilution) in (Cat no:21898-1-AP)

Yasuyo (Verified Customer) (05-24-2022)

Works great!!!

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: lung
TGF Beta 1 Antibody Immunohistochemistry validation (1:250 dilution) in lung (Cat no:21898-1-AP)

P (Verified Customer) (12-01-2021)


  • Applications: Western Blot
  • Cell Tissue Type: fibroblast
TGF Beta 1 Antibody Western Blot validation ( dilution) in fibroblast (Cat no:21898-1-AP)

Iram (Verified Customer) (09-04-2020)

Very clean bands for western

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000 for western
  • Cell Tissue Type: Human colon cancer

Qingyuan (Verified Customer) (11-05-2019)

The antibody clearly shows one band with the right molecular weight size.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse spinal cord