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TGF-beta 1 Antibody

TGF-beta 1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21898-1-AP

Synonyms

CED, DPD1, LAP, TGF beta 1, tgf beta1, TGFB, TGFB1, TGFbeta, tgfbeta1, tgf-beta1, TGFβ, TGFβ1, TGF-β1



Tested Applications

Positive WB detected inmouse skin tissue, K-562 cells, HeLa cells, A549 cells, rat skin tissue
Positive IHC detected inhuman placenta tissue, human prostate cancer tissue, mouse ovary tissue, mouse spleen tissue, rat ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 21898-1-AP immunogen is Fusion Protein TGF-beta 1 protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity dog, human, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TGF-beta 1 fusion protein Ag13591
Full Name transforming growth factor, beta 1
Calculated molecular weight 44 kDa
Observed molecular weight 44 kDa
GenBank accession number BC000125
Gene symbol TGFB1
Gene ID (NCBI) 7040
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1). Full length, inactive 44 kDa TGFB1 is cleaved into mature TGFB1 (13 kDa). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.

Protocols

Product Specific Protocols
WB protocol for TGF-beta 1 antibody 21898-1-APDownload protocol
IHC protocol for TGF-beta 1 antibody 21898-1-APDownload protocol
IF protocol for TGF-beta 1 antibody 21898-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biomed Res Int

Gossypol Promotes Bone Formation in Ovariectomy-Induced Osteoporosis through Regulating Cell Apoptosis.

Authors - Jinqian Liang
humanWB

Cell Signal

Activated CRH receptors inhibit autophagy by repressing conversion of LC3BI to LC3BII.

Authors - Lai Jin
humanWB

EBioMedicine

miR-4739 mediates pleural fibrosis by targeting bone morphogenetic protein 7.

Authors - Meng Wang
human

Nucleic Acids Res

Genetic screens reveal mechanisms for the transcriptional regulation of tissue-specific genes in normal cells and tumors.

Authors - Ikrame Naciri
mouseWB

Mol Med Rep

Astaxanthin ameliorates renal interstitial fibrosis and peritubular capillary rarefaction in unilateral ureteral obstruction.

Authors - Jin Zhao
ratWB

Eur J Pharmacol

Rosiglitazone attenuates paraquat-induced lung fibrosis in rats in a PPAR gamma-dependent manner.

Authors - Honglei Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Qingyuan (Verified Customer) (11-05-2019)

The antibody clearly shows one band with the right molecular weight size.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse spinal cord
No image submitted with review