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  • KD/KO Validated

TGF beta 2-Specific Polyclonal antibody

TGF beta 2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 19999-1-AP

Synonyms

BSC 1 cell growth inhibitor, Cetermin, G TSF, Polyergin, TGF beta 2, TGF beta 2-Specific, TGF beta2, TGFB2, TGF-beta 2, TGFβ2, TGF-β2



Tested Applications

Positive WB detected inHeLa cells, K-562 cells, mouse lung tissue, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19999-1-AP targets TGF beta 2-Specific in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name transforming growth factor, beta 2
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberNM_003238
Gene Symbol TGFB2
Gene ID (NCBI) 7042
RRIDAB_10646467
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tranforming growth factor beta-2 (TGFB2), also named G-TSF, BSC-1 cell growth inhibitor, Polyergin and Cetermin, belongs to the TGF-beta family. TGFB2 is a secreted protein and contains a 19aa signal peptides at its N-terminal. Heterodimers with TGFB1 and with TGFB2 have been found in bone. It was proved to have suppressive effects on interleukin-2 dependent T-cell growth. This antibody is TGFB2 specific not detecting other TGFBs.

Protocols

Product Specific Protocols
WB protocol for TGF beta 2-Specific antibody 19999-1-APDownload protocol
IHC protocol for TGF beta 2-Specific antibody 19999-1-APDownload protocol
IF protocol for TGF beta 2-Specific antibody 19999-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

ACS Nano

Gold Nanoparticles Induce Tumor Vessel Normalization and Impair Metastasis by Inhibiting Endothelial Smad2/3 Signaling.

Authors - Na Huang
humanWB,IF

Cancer Res

Osteoblast-secreted factors mediate dormancy of metastatic prostate cancer in the bone via activation of the TGFβRIII-p38MAPK-pS249/T252RB pathway.

Authors - Li-Yuan Yu-Lee
  • KD Validated
humanWB

Cell Death Differ

USP9X-mediated KDM4C deubiquitination promotes lung cancer radioresistance by epigenetically inducing TGF-β2 transcription.

Authors - Xiaohua Jie
humanWB

Dev Cell

Endothelial-to-Osteoblast Conversion Generates Osteoblastic Metastasis of Prostate Cancer.

Authors - Song-Chang Lin
humanIHC

Cancer Res

SIX1 promotes tumor lymphangiogenesis by coordinating TGFβ signals that increase expression of VEGF-C.

Authors - Dan Liu
humanWB

Cancer Lett

ZEB1 represses biogenesis of circ-DOCK5 to facilitate metastasis in esophageal squamous cell carcinoma via a positive feedback loop with TGF-β.

Authors - Lingjiao Meng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Kazu (Verified Customer) (12-14-2022)

A clear, single band at the expected molecular weight was observed by western blotting.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: Feline ocular tissue