Tested Applications
| Positive WB detected in | HeLa cells, K-562 cells, mouse lung tissue, MCF-7 cells, MDA-MB-231 cells |
| Positive IHC detected in | human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19999-1-AP targets TGF beta 2-Specific in WB, IHC, IF/ICC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, bovine, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | transforming growth factor, beta 2 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | NM_003238 |
| Gene Symbol | TGFB2 |
| Gene ID (NCBI) | 7042 |
| RRID | AB_10646467 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P61812 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tranforming growth factor beta-2 (TGFB2), also named G-TSF, BSC-1 cell growth inhibitor, Polyergin and Cetermin, belongs to the TGF-beta family. TGFB2 is a secreted protein and contains a 19aa signal peptides at its N-terminal. Heterodimers with TGFB1 and with TGFB2 have been found in bone. It was proved to have suppressive effects on interleukin-2 dependent T-cell growth. This antibody is TGFB2 specific not detecting other TGFBs.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TGF beta 2-Specific antibody 19999-1-AP | Download protocol |
| IHC protocol for TGF beta 2-Specific antibody 19999-1-AP | Download protocol |
| WB protocol for TGF beta 2-Specific antibody 19999-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TGF-β2 antibody (Cat# 19999-1-AP) has 86 citations published in journals including Nature Immunology, Nature Communications, Cancer Research, Cell Death & Differentiation, EMBO Journal, Gut, Development, Stem Cells, and ACS Nano. Research fields encompass TGF-β signaling, fibrosis, oncology (gastric, colorectal, lung, glioma, breast), cardiovascular disease, ophthalmology, immunology, and developmental biology. Primary applications include Western blot, IHC, IF, CoIP, and ChIP.
| Species | Application | Title |
|---|---|---|
Nat Immunol Spatial patterning of fibroblast TGFβ signaling underlies treatment resistance in rheumatoid arthritis. | ||
Gut ISG15 deficiency in hepatic stellate cells promotes TGFβ2-induced liver fibrosis by counteracting CREB1 ISGylation | ||
Cancer Res Osteoblast-secreted factors mediate dormancy of metastatic prostate cancer in the bone via activation of the TGFβRIII-p38MAPK-pS249/T252RB pathway.
| ||
Cancer Res SIX1 promotes tumor lymphangiogenesis by coordinating TGFβ signals that increase expression of VEGF-C. | ||
Nat Commun Intermittent fasting inhibits Tp53-driven glioma through gut microbiota-mediated methionine-m(6)A regulation. | ||
Circ Res Endothelial Loss of ETS1 Impairs Coronary Vascular Development and Leads to Ventricular Non-Compaction |
Reviews
What customers say
Both reviewers rated the antibody 5 stars for Western Blot. One user (1:1000 dilution) detected clear expression at the expected 48 kDa molecular weight in mouse kidney and spleen lysates, along with a 12 kDa monomer isoform in spleen. The other user (1:400 dilution) observed a clear, single band at the expected molecular weight in feline ocular tissue. Overall, the antibody is well-regarded for producing specific, expected-size bands across different species and tissue types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Aline Seiko (Verified Customer) (05-12-2025) | It was observed a clear expression of the protein in the expected molecular weight of 48kDa, but also for the spleen it was observed a monomer isoform of 12kDa.
|
Kazu (Verified Customer) (12-14-2022) | A clear, single band at the expected molecular weight was observed by western blotting.
|
