TGFBR3-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

TGFBR3-Specific Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 20000-1-AP

Synonyms

betaglycan, BGCAN, TGF beta receptor type 3, TGF beta receptor type III, TGF-beta Receptor III, TGFBR3, TGFBR3-Specific, TGFR 3, TGFβRIII

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Validation Data Gallery

• 

  WB analysis of A549 using 20000-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20000-1-AP (TGFBR3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 20000-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20000-1-AP (TGFBR3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  FC experiment of A549 using 20000-1-AP

  1X10^6 A549 cells were stained with 0.2ug TGFBR3-Specific antibody (20000-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: A549 cells, A431 cells
• Positive FC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

20000-1-AP targets TGFBR3-Specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: transforming growth factor, beta receptor III
• Calculated molecular weight: 93 kDa
• Observed molecular weight: 110-150 kDa
• GenBank accession number: NM_003243
• Gene symbol: TGFBR3
• Gene ID (NCBI): 7049
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TGFBR3, also named as TGFR-3, BGCAN and betaglycan, binds to TGF-beta. TGFBR3 could be involved in capturing and retaining TGF-beta for presentation to the signaling receptors. This antibody is specific to TGFBR3.

Publications

Species	Application	Title
goat	IHC	Cell Prolif; Spatially multicellular variability of intervertebral disc degeneration by comparative single-cell analysis; Authors - Peng Lin
mouse		Int Immunopharmacol; Vitamin D3 alleviates pulmonary fibrosis by regulating the MAPK pathway via targeting PSAT1 expression in vivo and in vitro.; Authors - Wenxiang Zhu
human	WB	Mol Cell Endocrinol; The miR-103a-3p/TGFBR3 axis regulates TGF-β-induced orbital fibroblast activation and fibrosis in thyroid-eye disease; Authors - Bingyu Xie ; KD Validated
human	WB,IF	Cancer Res; Osteoblast-secreted factors mediate dormancy of metastatic prostate cancer in the bone via activation of the TGFβRIII-p38MAPK-pS249/T252RB pathway.; Authors - Li-Yuan Yu-Lee ; KD Validated
mouse	WB	Redox Biol; FoxO1 regulates adipose transdifferentiation and iron influx by mediating Tgfβ1 signaling pathway; Authors - Limin Shi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Manfred (Verified Customer) (05-09-2022)

I highly recommend this anitbody. Protein of interest was detected at 94kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: PBMCs