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  • KD/KO Validated

TGFBR3-Specific Polyclonal antibody

TGFBR3-Specific Polyclonal Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 20000-1-AP


betaglycan, BGCAN, TGF beta receptor type 3, TGF beta receptor type III, TGF-beta Receptor III, TGFBR3, TGFBR3-Specific, TGFR 3, TGFβRIII, transforming growth factor, beta

Tested Applications

Positive WB detected inA549 cells, A431 cells
Positive FC detected inA549 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20000-1-AP targets TGFBR3-Specific in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name transforming growth factor, beta receptor III
Calculated Molecular Weight 93 kDa
Observed Molecular Weight 110-150 kDa
GenBank Accession NumberNM_003243
Gene Symbol TGFBR3
Gene ID (NCBI) 7049
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transforming growth factor β (TGF-β) is a multifunctional cytokine that plays important roles in normal development and homeostasis (PMID: 8530052). There are three TGF-β forms: TGF-β1, TGF-β2, and TGF-β3. The diverse effects of TGF-β are mediated by the TGF-β receptors and cell surface-binding proteins. Three TGF-beta receptors exist: TGFBR1, TFGBR2, TGFBR3 (PMID: 8530052; 30184463). TGFBR3, also known as betaglycan, is a transmembrane proteoglycan that often functions as a co-receptor with other TGF-beta receptor superfamily members. In addition to the transmembrane form, the extracellular domain of TGFBR3 can be cleaved to a soluble form (sTGFBR3), which may serve as an antagonist of TGF-β to inhibit TGF-β signaling (PMID: 30184463). TGFBR3 has a larger apparent molecular weight than the calculated molecular weight, due to extensive carbohydrate modifications (PMID: 1556106). This antibody is specific to TGFBR3.


Product Specific Protocols
WB protocol for TGFBR3-Specific antibody 20000-1-APDownload protocol
FC protocol for TGFBR3-Specific antibody 20000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cancer Res

Osteoblast-secreted factors mediate dormancy of metastatic prostate cancer in the bone via activation of the TGFβRIII-p38MAPK-pS249/T252RB pathway.

Authors - Li-Yuan Yu-Lee
  • KD Validated

Cell Prolif

Spatially multicellular variability of intervertebral disc degeneration by comparative single-cell analysis

Authors - Peng Lin

Int Immunopharmacol

Vitamin D3 alleviates pulmonary fibrosis by regulating the MAPK pathway via targeting PSAT1 expression in vivo and in vitro.

Authors - Wenxiang Zhu

Mol Cell Endocrinol

The miR-103a-3p/TGFBR3 axis regulates TGF-β-induced orbital fibroblast activation and fibrosis in thyroid-eye disease

Authors - Bingyu Xie
  • KD Validated

Redox Biol

FoxO1 regulates adipose transdifferentiation and iron influx by mediating Tgfβ1 signaling pathway

Authors - Limin Shi

Biochem Biophys Res Commun

CUL4B enhances the malignant phenotype of esophageal squamous cell carcinoma by suppressing TGFBR3 expression

Authors - Qi Gong


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Kenzo (Verified Customer) (08-15-2023)

This antibody worked well for labeling TGF beta receptor type 3 in mouse kidney. I was able to acquire robust immunofluorescence signals with the antibody and highly recommend this KO validated antibody.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse kidney

Manfred (Verified Customer) (05-09-2022)

I highly recommend this anitbody. Protein of interest was detected at 94kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: PBMCs