TGN46 Polyclonal antibody

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  WB analysis using 13573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13573-1-AP (TGN46 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13573-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13573-1-AP (TGN46 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13573-1-AP

  IP result of anti-TGN46 (IP:13573-1-AP, 3ug; Detection:13573-1-AP 1:300) with HeLa cells lysate 2400ug.

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  IP experiment of HeLa using 13573-1-AP

  IP result of anti-TGN46 (IP:13573-1-AP, 3ug; Detection:13573-1-AP 1:300) with HeLa cells lysate 2400ug.

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  IHC staining of human stomach cancer using 13573-1-AP

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 13573-1-AP (TGN46 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human cerebellum using 13573-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human cerebellum tissue using 13573-1-AP (TGN46 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 13573-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TGN46 antibody (13573-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 13573-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13573-1-AP (TGN46 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL555-phalloidin stains F-actin (red).

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  IF Staining of A549 using 13573-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TGN46 antibody (13573-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of Fibroblast (primary cells) using 13573-1-AP

  IF result of TGN46 antibody (1:50) with Human Fibroblast (primary cells) By Dr. Neeraj Tiwar, Rothman Lab, Yale School of Medicine. Fixed with 4% PFA 10 min.

TGN46 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 13573-1-AP

Publications(38)

Reviews (5)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

TGOLN2, TGN48, TGN38 homolog, TGN38, hTGN51

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach cancer; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human cerebellum tissue
• Positive IF/ICC detected in: HeLa cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IF: See 28 publications below

Product Information

13573-1-AP targets TGN46 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, pig, monkey, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TGN46 fusion protein Ag4470
• Full Name: trans-golgi network protein 2
• Calculated Molecular Weight: 447 aa, 47 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC028219
• Gene Symbol: TGN46
• Gene ID (NCBI): 10618
• RRID: AB_10597396
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43493
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TGN46 (TGOLN2), the human homolog of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively, spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		ACS Cent Sci; Biowire Model of Interstitial and Focal Cardiac Fibrosis.; Authors - Erika Yan Wang
human	IF	Autophagy; Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.; Authors - Amy H Ponsford
human	IF	Cell Death Differ; Oleate-induced aggregation of LC3 at the trans-Golgi network is linked to a protein trafficking blockade.; Authors - Giulia Cerrato
human	WB	Autophagy; Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress; Authors - Jia-min Yan
human	IF	EMBO Rep; A conserved ion channel function of STING mediates noncanonical autophagy and cell death; Authors - Jinrui Xun
human	IF	PLoS Pathog; Host RAB11FIP5 protein inhibits the release of Kaposi's sarcoma-associated herpesvirus particles by promoting lysosomal degradation of ORF45.; Authors - Xiaoqin Wei

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sammy (Verified Customer) (01-20-2024)

A good antibody for IF and also works for WB.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: HUVEC

FH

Amy (Verified Customer) (08-10-2023)

Nice Golgi staining of HEK293T cells.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HEK293T

FH

Christine (Verified Customer) (01-27-2023)

Detects 2 bands around the 85 kDa marker, a sharp one just below and a more fuzzy one just above.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2,500
• Cell Tissue Type: HEK293T

FH

Thomas (Verified Customer) (09-18-2020)

HEK293T cells were fixed in 4% PFA for 15 mins and permeabilised in 0.1% Triton-X 100 in PBS. Cells were blocked in 1% BSA. Primary antibody solution was diluted at 1:200 in blocking solution and incubated for 1 hour. Goat anti-rabbit 647 Alexa Fluor secondary antibody (1:250 - red) and DAPI (1:2000 - blue) were incubated with cells for 1 hour.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200

TGN46 Antibody Immunofluorescence validation (1:200 dilution) in (Cat no:13573-1-AP)

FH

Stephen (Verified Customer) (10-01-2019)

RPE cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours TGN46 antibody(green) incubated 1:300 and EEA1 antibody (purple) overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) visualize DNA

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: RPE (human)

TGN46 Antibody Immunofluorescence, validation (1:300 dilution) in RPE (human) (Cat no:13573-1-AP)