Validation Data Gallery
|Positive WB detected in||A549 cells, HeLa cells, HepG2 cells|
|Positive IP detected in||HeLa cells|
|Positive IF detected in||HeLa cells, A549 cells, Fibroblast (primary cells), human cerebellum tissue|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13573-1-AP targets TGN46 in WB, IP, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TGN46 fusion protein Ag4470|
|Full Name||trans-golgi network protein 2|
|Calculated molecular weight||447 aa, 47 kDa|
|Observed molecular weight||90-100 kDa|
|GenBank accession number||BC028219|
|Gene ID (NCBI)||10618|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Thomas (Verified Customer) (09-18-2020)
HEK293T cells were fixed in 4% PFA for 15 mins and permeabilised in 0.1% Triton-X 100 in PBS. Cells were blocked in 1% BSA. Primary antibody solution was diluted at 1:200 in blocking solution and incubated for 1 hour. Goat anti-rabbit 647 Alexa Fluor secondary antibody (1:250 - red) and DAPI (1:2000 - blue) were incubated with cells for 1 hour.
Stephen (Verified Customer) (10-01-2019)
RPE cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours TGN46 antibody(green) incubated 1:300 and EEA1 antibody (purple) overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) visualize DNA