TGN46 Polyclonal antibody

TGN46 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

13

Reviews (2)

Cat no : 13573-1-AP

Synonyms

Tgn38, TGN38 homolog, TGN46, TGN48, TGN51, TGOLN2, trans golgi network protein 2, TTGN2

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Validation Data Gallery

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  WB analysis using 13573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13573-1-AP (TGN46 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13573-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13573-1-AP (TGN46 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13573-1-AP

  IP Result of anti-TGN46 (IP:13573-1-AP, 3ug; Detection:13573-1-AP 1:300) with HeLa cells lysate 2400ug.

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  IP experiment of HeLa using 13573-1-AP

  IP Result of anti-TGN46 (IP:13573-1-AP, 3ug; Detection:13573-1-AP 1:300) with HeLa cells lysate 2400ug.

• 

  IF Staining of HeLa using 13573-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13573-1-AP (TGN46 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL555-phalloidin stains F-actin (red).

• 

  IF Staining of Fibroblast (primary cells) using 13573-1-AP

  IF result of TGN46 antibody (1:50) with Human Fibroblast (primary cells) By Dr. Neeraj Tiwar, Rothman Lab, Yale School of Medicine. Fixed with 4% PFA 10 min.

• 

  IF Staining of human cerebellum using 13573-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human cerebellum tissue using 13573-1-AP (TGN46 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human cerebellum using 13573-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human cerebellum tissue using 13573-1-AP (TGN46 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 13573-1-AP

  Immunofluorescent analysis of A549 cells using 13573-1-AP (TGN46 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IF detected in: HeLa cells, A549 cells, Fibroblast (primary cells), human cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IF: See 10 publications below

Product Information

13573-1-AP targets TGN46 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TGN46 fusion protein Ag4470
• Full Name: trans-golgi network protein 2
• Calculated molecular weight: 447 aa, 47 kDa
• Observed molecular weight: 90-100 kDa
• GenBank accession number: BC028219
• Gene symbol: TGOLN2
• Gene ID (NCBI): 10618
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.

Publications

Species	Application	Title
human	IF	Autophagy; Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.; Authors - Amy H Ponsford
human	IF	Cell Death Differ; Oleate-induced aggregation of LC3 at the trans-Golgi network is linked to a protein trafficking blockade.; Authors - Giulia Cerrato
human	WB	Autophagy; Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress; Authors - Jia-min Yan
human	IF	PLoS Pathog; Host RAB11FIP5 protein inhibits the release of Kaposi's sarcoma-associated herpesvirus particles by promoting lysosomal degradation of ORF45.; Authors - Xiaoqin Wei
human	WB	Oncogenesis; GP73-mediated secretion of AFP and GP73 promotes proliferation and metastasis of hepatocellular carcinoma cells.; Authors - Yujuan Liu
human	IF	Mol Ther Nucleic Acids; An Efficient Bivalent Cyclic RGD-PIK3CB siRNA Conjugate for Specific Targeted Therapy against Glioblastoma In Vitro and In Vivo.; Authors - Bohong Cen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Thomas (Verified Customer) (09-18-2020)

HEK293T cells were fixed in 4% PFA for 15 mins and permeabilised in 0.1% Triton-X 100 in PBS. Cells were blocked in 1% BSA. Primary antibody solution was diluted at 1:200 in blocking solution and incubated for 1 hour. Goat anti-rabbit 647 Alexa Fluor secondary antibody (1:250 - red) and DAPI (1:2000 - blue) were incubated with cells for 1 hour.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200

TGN46 Antibody Immunofluorescence validation (1:200 dilution) in (Cat no:13573-1-AP)

FH

Stephen (Verified Customer) (10-01-2019)

RPE cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours TGN46 antibody(green) incubated 1:300 and EEA1 antibody (purple) overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) visualize DNA

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: RPE (human)

TGN46 Antibody Immunofluorescence, validation (1:300 dilution) in RPE (human) (Cat no:13573-1-AP)

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