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TGN46 Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 13573-1-AP

1 customer review

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Con: 50 μg/150 μl

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Tested Applications

Applications:
WB, IP, IF, ELISA
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TGN46 Antibody IF A549 cells 13573-1-AP

Immunofluorescent analysis of A549 cells using 13573-1-AP (TGN46 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

TGN46 Antibody IF Fibroblast (primary cells) 13573-1-AP

IF result of TGN46 antibody (1:50) with Human Fibroblast (primary cells) By Dr. Neeraj Tiwar, Rothman Lab, Yale School of Medicine. Fixed with 4% PFA 10 min.

TGN46 Antibody WB A549 cells 13573-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 13573-1-AP (TGN46 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours

TGN46 Antibody IP HeLa cells 13573-1-AP

IP Result of anti-TGN46 (IP:13573-1-AP, 3ug; Detection:13573-1-AP 1:300) with HeLa cells lysate 2400ug.

Species specificity:
human
Positive WB detected in:
A549 cells, HeLa cells, HepG2 cells
Positive IP detected in:
HeLa cells
Positive IF detected in:
A549 cells, HeLa cells, Fibroblast (primary cells)
Recommended dilution:
WB : 1:1000-1:8000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
TGN46 fusion protein Ag4470
Full name:
trans-golgi network protein 2
Calculated molecular weight:
447aa,47 kDa
Observed molecular weight:
90-100 kDa
GenBank accession number:
BC028219
Gene ID (NCBI):
10618
Gene symbol
TGOLN2
Synonyms
Tgn38, TGN38 homolog, TGN46, TGN48, TGN51, TGOLN2, trans golgi network protein 2, TTGN2

Background

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

TGN46 Rabbit Polyclonal antibody

Stephen (Verified Customer), 10-01-2019
Applications
Immunofluorescence,
Primary Antibody Dilution
1:300
TGN46 Antibody Immunofluorescence, validation (1:300 dilution) in RPE (human) (Cat no:13573-1-AP)

TGN46 Antibody Immunofluorescence, validation (1:300 dilution) in RPE (human) (Cat no:13573-1-AP)

Cell/Tissue Type
RPE (human)
Lot Number
RPE cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours TGN46 antibody(green) incubated 1:300 and EEA1 antibody (purple) overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) visualize DNA

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