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TGN46 Polyclonal antibody

TGN46 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 13573-1-AP

Synonyms

Tgn38, TGN38 homolog, TGN46, TGN48, TGN51, TGOLN2, trans golgi network protein 2, TTGN2

Validation Data Gallery

  • IF Staining of HeLa using 13573-1-AP

    IF Staining of HeLa using 13573-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13573-1-AP (TGN46 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL555-phalloidin stains F-actin (red).

  • IF Staining of Fibroblast (primary cells) using 13573-1-AP

    IF Staining of Fibroblast (primary cells) using 13573-1-AP

    IF result of TGN46 antibody (1:50) with Human Fibroblast (primary cells) By Dr. Neeraj Tiwar, Rothman Lab, Yale School of Medicine. Fixed with 4% PFA 10 min.

  • WB analysis using 13573-1-AP

    WB analysis using 13573-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13573-1-AP (TGN46 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 13573-1-AP

    IP experiment of HeLa using 13573-1-AP

    IP Result of anti-TGN46 (IP:13573-1-AP, 3ug; Detection:13573-1-AP 1:300) with HeLa cells lysate 2400ug.

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells, A549 cells, human cerebellum tissue, Fibroblast (primary cells)

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 6 publications below

Product Information

13573-1-AP targets TGN46 in WB, IP, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TGN46 fusion protein Ag4470
Full Name trans-golgi network protein 2
Calculated molecular weight 447 aa, 47 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC028219
Gene symbol TGOLN2
Gene ID (NCBI) 10618
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.

Protocols

Product Specific Protocols
WB protocol for TGN46 antibody 13573-1-APDownload protocol
IF protocol for TGN46 antibody 13573-1-APDownload protocol
IP protocol for TGN46 antibody 13573-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

ACS Cent Sci

Biowire Model of Interstitial and Focal Cardiac Fibrosis.

Authors - Erika Yan Wang
View Article
humanIF

Int J Mol Sci

Novel Insights into Selected Disease-Causing Mutations within the SLC35A1 Gene Encoding the CMP-Sialic Acid Transporter.

Authors - Bożena Szulc
View Article
humanIF

mBio

Intracellular Group A Streptococcus Induces Golgi Fragmentation To Impair Host Defenses through Streptolysin O and NAD-Glycohydrolase.

Authors - Takashi Nozawa
View Article
humanIF

J Proteomics

Identification of novel potential interaction partners of UDP-galactose (SLC35A2), UDP-N-acetylglucosamine (SLC35A3) and an orphan (SLC35A4) nucleotide sugar transporters.

Authors - Maciej Wiktor
View Article
humanIF

Mol Ther Nucleic Acids

An Efficient Bivalent Cyclic RGD-PIK3CB siRNA Conjugate for Specific Targeted Therapy against Glioblastoma In Vitro and In Vivo.

Authors - Bohong Cen
View Article
humanIF

PLoS Pathog

Host RAB11FIP5 protein inhibits the release of Kaposi's sarcoma-associated herpesvirus particles by promoting lysosomal degradation of ORF45.

Authors - Xiaoqin Wei
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Thomas (Verified Customer) (09-18-2020)

HEK293T cells were fixed in 4% PFA for 15 mins and permeabilised in 0.1% Triton-X 100 in PBS. Cells were blocked in 1% BSA. Primary antibody solution was diluted at 1:200 in blocking solution and incubated for 1 hour. Goat anti-rabbit 647 Alexa Fluor secondary antibody (1:250 - red) and DAPI (1:2000 - blue) were incubated with cells for 1 hour.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
TGN46 Antibody Immunofluorescence validation (1:200 dilution) in (Cat no:13573-1-AP)
FH

Stephen (Verified Customer) (10-01-2019)

RPE cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours TGN46 antibody(green) incubated 1:300 and EEA1 antibody (purple) overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) visualize DNA

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: RPE (human)
TGN46 Antibody Immunofluorescence, validation (1:300 dilution) in RPE (human) (Cat no:13573-1-AP)