TGN46 Monoclonal antibody
TGN46 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat, Pig, Mouse and More (3)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1F6D5
Cat no : 66477-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, LNCaP cells, HepG2 cells, L02 cells, SMMC-7721 cells, human colostrum tissue, pig liver tissue |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| IF | See 5 publications below |
Product Information
66477-1-Ig targets TGN46 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Rat, Pig, Mouse samples.
| Tested Reactivity | Human, Rat, Pig, Mouse |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TGN46 fusion protein Ag4470 |
| Full Name | trans-golgi network protein 2 |
| Calculated molecular weight | 447 aa, 47 kDa |
| Observed molecular weight | 90 kDa |
| GenBank accession number | BC028219 |
| Gene symbol | TGOLN2 |
| Gene ID (NCBI) | 10618 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TGN46 antibody 66477-1-Ig | Download protocol |
| IHC protocol for TGN46 antibody 66477-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Vet Microbiol Porcine deltacoronavirus accessory protein NS7a possesses the functional characteristics of a viroporin | ||
Cell Res Targeting ATAD3A-PINK1-mitophagy axis overcomes chemoimmunotherapy resistance by redirecting PD-L1 to mitochondria | ||
Cell Rep The ubiquitin E3 ligase TRIM10 promotes STING aggregation and activation in the Golgi apparatus | ||
Hum Mol Genet Activation of the cGAS-STING innate immune response in cells with deficient mitochondrial topoisomerase TOP1MT | ||
J Biol Chem Human V-ATPase a-subunit isoforms bind specifically to distinct phosphoinositide phospholipids |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Karla (Verified Customer) (12-20-2022) | Proteintech has the best for IMF. Made my images clear and was picked up easily on the microscope.
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