TGN46 Monoclonal antibody

TGN46 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig, Mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1F6D5

Cat no : 66477-1-Ig

Synonyms

TGN38, TGN38 homolog, TGN46, TGN48, TGN51, TGOLN2, trans golgi network protein 2, TTGN2

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Validation Data Gallery

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  WB analysis of HeLa using 66477-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66477-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66477-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66477-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66477-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-12 using 66477-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using 66477-1-Ig (TGN46 antibody) at dilution of 1:100 and 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:50, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66477-1-Ig, and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 10841-1-AP.

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  IF Staining of A549 using 66477-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using 66477-1-Ig (TGN46 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 66477-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66477-1-Ig (TGN46 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, SMMC-7721 cells, pig liver tissue
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: PC-12 cells, A549 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• IF: See 1 publications below

Product Information

66477-1-Ig targets TGN46 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Rat, Pig, Mouse samples.

• Tested Reactivity: Human, Rat, Pig, Mouse
• Cited Reactivity: pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TGN46 fusion protein Ag4470
• Full Name: trans-golgi network protein 2
• Calculated molecular weight: 447 aa, 47 kDa
• Observed molecular weight: 90 kDa
• GenBank accession number: BC028219
• Gene symbol: TGOLN2
• Gene ID (NCBI): 10618
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.

Publications

Species	Application	Title
pig	IF	Vet Microbiol; Porcine deltacoronavirus accessory protein NS7a possesses the functional characteristics of a viroporin; Authors - Sijin Xia

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