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TGN46 Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66477-1-Ig |CloneNo.: 1F6D5

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

TGN46 Antibody WB HeLa cells 66477-1-Ig

HeLa cells were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

TGN46 Antibody IF PC-12 cells 66477-1-Ig

Immunofluorescent analysis of ( 4% PFA) fixed PC-12 cells using 66477-1-Ig (TGN46 antibody) at dilution of 1:100 and 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:50, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66477-1-Ig, and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 10841-1-AP.

TGN46 Antibody IHC human breast cancer tissue 66477-1-Ig

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TGN46 Antibody IHC human breast cancer tissue 66477-1-Ig

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
Human, Rat, Pig
Positive WB detected in:
HeLa cells, HepG2 cells, SMMC-7721 cells, pig liver tissue
Positive IHC detected in:
human breast cancer tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
PC-12 cells
Recommended dilution:
WB : 1:1000-1:6000
IHC : 1:150-1:600
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
TGN46 fusion protein Ag4470
Full name:
trans-golgi network protein 2
Calculated molecular weight:
447aa,47 kDa
Observed molecular weight:
90 kDa
GenBank accession number:
BC028219
Gene ID (NCBI):
10618
Gene symbol
TGOLN2
Synonyms
TGN38, TGN38 homolog, TGN46, TGN48, TGN51, TGOLN2, trans golgi network protein 2, TTGN2

Background

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.


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