TGN46 Monoclonal antibody
TGN46 Monoclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat, Pig, Mouse
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
CloneNo.
1F6D5
Cat no : 66477-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HepG2 cells, SMMC-7721 cells, pig liver tissue |
Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | PC-12 cells, A549 cells, mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
66477-1-Ig targets TGN46 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Rat, Pig, Mouse samples.
Tested Reactivity | Human, Rat, Pig, Mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | TGN46 fusion protein Ag4470 |
Full Name | trans-golgi network protein 2 |
Calculated molecular weight | 447 aa, 47 kDa |
Observed molecular weight | 90 kDa |
GenBank accession number | BC028219 |
Gene symbol | TGOLN2 |
Gene ID (NCBI) | 10618 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.
Protocols
Product Specific Protocols | |
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WB protocol for TGN46 antibody 66477-1-Ig | Download protocol |
IHC protocol for TGN46 antibody 66477-1-Ig | Download protocol |
IF protocol for TGN46 antibody 66477-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |