TGN46 Monoclonal antibody

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  WB analysis of HeLa using 66477-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66477-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66477-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66477-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66477-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66477-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TGN46 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66477-1-Ig

Clone No.1F6D5

Publications(4)

Reviews (1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Synonyms

TGOLN2, 1F6D5, hTGN46, hTGN48, hTGN51

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, LNCaP cells, HepG2 cells, L02 cells, SMMC-7721 cells, human colostrum tissue, pig liver tissue
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66477-1-Ig targets TGN46 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TGN46 fusion protein Ag4470
• Full Name: trans-golgi network protein 2
• Calculated Molecular Weight: 447 aa, 47 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC028219
• Gene Symbol: TGN46
• Gene ID (NCBI): 10618
• RRID: AB_2881843
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O43493
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.

Publications

Species	Application	Title
human		Arterioscler Thromb Vasc Biol; IL-1β Induces LDL Transcytosis by a Novel Pathway Involving LDLR and Rab27a; Authors - Erika Jang
pig		J Virol; Porcine deltacoronavirus accessory protein NS6 harnesses VPS35-mediated retrograde trafficking to facilitate efficient viral infection; Authors - Puxian Fang
mouse		J Cell Physiol; Locational and functional characterization of PI4KB in the mouse embryo; Authors - Haoyu Zhao
mouse		Int J Biol Sci; Annexin A5 Derived from Cell-free Fat Extract Attenuates Osteoarthritis via Macrophage Regulation; Authors - Zhuoxuan Jia

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Karla (Verified Customer) (12-20-2022)

Proteintech has the best for IMF. Made my images clear and was picked up easily on the microscope.

• Applications: Immunofluorescence