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TGN46 Monoclonal antibody

TGN46 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig, Mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1F6D5

Cat no : 66477-1-Ig

Synonyms

TGN38, TGN38 homolog, TGN46, TGN48, TGN51, TGOLN2, trans golgi network protein 2, TTGN2

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 66477-1-Ig

    WB analysis of HeLa using 66477-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 66477-1-Ig

    IHC staining of human breast cancer using 66477-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 66477-1-Ig

    IHC staining of human breast cancer using 66477-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66477-1-Ig

    IHC staining of human liver cancer using 66477-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66477-1-Ig

    IHC staining of human liver cancer using 66477-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-12 using 66477-1-Ig

    IF Staining of PC-12 using 66477-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using 66477-1-Ig (TGN46 antibody) at dilution of 1:100 and 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:50, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66477-1-Ig, and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 10841-1-AP.

  • IF Staining of A549 using 66477-1-Ig

    IF Staining of A549 using 66477-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using 66477-1-Ig (TGN46 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse brain using 66477-1-Ig

    IF Staining of mouse brain using 66477-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66477-1-Ig (TGN46 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, SMMC-7721 cells, pig liver tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-12 cells, A549 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66477-1-Ig targets TGN46 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Rat, Pig, Mouse samples.

Tested Reactivity Human, Rat, Pig, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TGN46 fusion protein Ag4470
Full Name trans-golgi network protein 2
Calculated molecular weight 447 aa, 47 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC028219
Gene symbol TGOLN2
Gene ID (NCBI) 10618
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.

Protocols

Product Specific Protocols
WB protocol for TGN46 antibody 66477-1-IgDownload protocol
IHC protocol for TGN46 antibody 66477-1-IgDownload protocol
IF protocol for TGN46 antibody 66477-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols