Validation Data Gallery
|Positive WB detected in||A549 cells, HeLa cells, LNCaP cells, HepG2 cells, L02 cells, SMMC-7721 cells, human colostrum tissue, pig liver tissue|
|Positive IHC detected in||human breast cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:150-1:600|
|Sample-dependent, check data in validation data gallery|
66477-1-Ig targets TGN46 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Rat, Pig, Mouse samples.
|Tested Reactivity||Human, Rat, Pig, Mouse|
|Cited Reactivity||human, mouse, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||TGN46 fusion protein Ag4470|
|Full Name||trans-golgi network protein 2|
|Calculated molecular weight||447 aa, 47 kDa|
|Observed molecular weight||90 kDa|
|GenBank accession number||BC028219|
|Gene ID (NCBI)||10618|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.
Porcine deltacoronavirus accessory protein NS7a possesses the functional characteristics of a viroporin
Targeting ATAD3A-PINK1-mitophagy axis overcomes chemoimmunotherapy resistance by redirecting PD-L1 to mitochondria
The ubiquitin E3 ligase TRIM10 promotes STING aggregation and activation in the Golgi apparatus
Hum Mol Genet
Activation of the cGAS-STING innate immune response in cells with deficient mitochondrial topoisomerase TOP1MT
J Biol Chem
Human V-ATPase a-subunit isoforms bind specifically to distinct phosphoinositide phospholipids
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Karla (Verified Customer) (12-20-2022)
Proteintech has the best for IMF. Made my images clear and was picked up easily on the microscope.