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- KD/KO Validated
THAP1 Polyclonal antibody
THAP1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IHC, ChIP
Cat no : 12584-1-AP
Validation Data Gallery
|Positive WB detected in||HepG2 cells, human brain tissue, HEK-293 cells, HeLa cells, human heart tissue, A375 cells, SGC-7901 cells, mouse brain tissue, mouse heart tissue|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 5 publications below|
|WB||See 11 publications below|
|IHC||See 1 publications below|
|ChIP||See 1 publications below|
12584-1-AP targets THAP1 in WB, IHC, ChIP applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||THAP1 fusion protein Ag3285|
|Full Name||THAP domain containing, apoptosis associated protein 1|
|Calculated molecular weight||213 aa, 25 kDa|
|Observed molecular weight||25-30 kDa|
|GenBank accession number||BC021721|
|Gene ID (NCBI)||55145|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
THAP1 belongs to the THAP1 family. It is a DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. THAP1 may also have pro-apoptopic activity by potentiating both serum-withdrawal and TNF-induced apoptosis. Mutations in THAP1 have been associated with dystonia 6. THAP1 encodes a transcription factor with mostly unknown targets. It regulates the expression of DYT1 (TOR1A), another dystonia-causing gene. After characterization of the TOR1A promoter, THAP1 binds to the core promoter of TOR1A. Wild type THAP1 represses the expression of TOR1A, whereas dystonia 6-associated mutant THAP1 results in decreased repression of TOR1A. Catalog#12584-1-AP is a rabbit polyclonal antibody raised against full-length of huamn THAP1.
|Product Specific Protocols|
|WB protocol for THAP1 antibody 12584-1-AP||Download protocol|
|IHC protocol for THAP1 antibody 12584-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mutations in the histone methyltransferase gene KMT2B cause complex early-onset dystonia.
The dystonia gene THAP1 controls DNA double-strand break repair choice.
DYT6 mutated THAP1 is a cell type dependent regulator of the SP1 family.
Direct interaction between causative genes of DYT1 and DYT6 primary dystonia.
The dystonia gene DYT1 is repressed by the transcription factor THAP1 (DYT6).
The DYT6 Dystonia Protein THAP1 Regulates Myelination within the Oligodendrocyte Lineage.