|Positive WB detected in||HEK-293 cells, A375 cells, HeLa cells, HepG2 cells, human brain tissue, human heart tissue, mouse brain tissue, mouse heart tissue, SGC-7901 cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
12584-1-AP targets THAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||THAP1 fusion protein Ag3285|
|Full Name||THAP domain containing, apoptosis associated protein 1|
|Calculated molecular weight||213 aa, 25 kDa|
|Observed molecular weight||25-30 kDa|
|GenBank accession number||BC021721|
|Gene ID (NCBI)||55145|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
THAP1 belongs to the THAP1 family. It is a DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. THAP1 may also have pro-apoptopic activity by potentiating both serum-withdrawal and TNF-induced apoptosis. Mutations in THAP1 have been associated with dystonia 6. THAP1 encodes a transcription factor with mostly unknown targets. It regulates the expression of DYT1 (TOR1A), another dystonia-causing gene. After characterization of the TOR1A promoter, THAP1 binds to the core promoter of TOR1A. Wild type THAP1 represses the expression of TOR1A, whereas dystonia 6-associated mutant THAP1 results in decreased repression of TOR1A. Catalog#12584-1-AP is a rabbit polyclonal antibody raised against full-length of huamn THAP1.
Acta Neuropathol Commun
Dystonia type 6 gene product Thap1: identification of a 50 kDa DNA-binding species in neuronal nuclear fractions.
Neural expression of the transcription factor THAP1 during development in rat.
Hum Mol Genet
Abnormalities of motor function, transcription and cerebellar structure in mouse models of THAP1 dystonia.
Nucleic Acids Res
Stress-induced changes in gene interactions in human cells.
The DYT6 Dystonia Protein THAP1 Regulates Myelination within the Oligodendrocyte Lineage.
Direct interaction between causative genes of DYT1 and DYT6 primary dystonia.