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  • KD/KO Validated

THAP1 Polyclonal antibody

THAP1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12584-1-AP

Synonyms

THAP1



Tested Applications

Positive WB detected inHEK-293 cells, A375 cells, HeLa cells, HepG2 cells, human brain tissue, human heart tissue, mouse brain tissue, mouse heart tissue, SGC-7901 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12584-1-AP targets THAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THAP1 fusion protein Ag3285
Full Name THAP domain containing, apoptosis associated protein 1
Calculated molecular weight 213 aa, 25 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC021721
Gene symbol THAP1
Gene ID (NCBI) 55145
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

THAP1 belongs to the THAP1 family. It is a DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. THAP1 may also have pro-apoptopic activity by potentiating both serum-withdrawal and TNF-induced apoptosis. Mutations in THAP1 have been associated with dystonia 6. THAP1 encodes a transcription factor with mostly unknown targets. It regulates the expression of DYT1 (TOR1A), another dystonia-causing gene. After characterization of the TOR1A promoter, THAP1 binds to the core promoter of TOR1A. Wild type THAP1 represses the expression of TOR1A, whereas dystonia 6-associated mutant THAP1 results in decreased repression of TOR1A. Catalog#12584-1-AP is a rabbit polyclonal antibody raised against full-length of huamn THAP1.

Protocols

Product Specific Protocols
WB protocol for THAP1 antibody 12584-1-APDownload protocol
IHC protocol for THAP1 antibody 12584-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse, humanWB

Acta Neuropathol Commun

Dystonia type 6 gene product Thap1: identification of a 50 kDa DNA-binding species in neuronal nuclear fractions.

Authors - Maitane Ortiz-Virumbrales
  • KD Validated
human, ratWB,IHC

Neuroscience

Neural expression of the transcription factor THAP1 during development in rat.

Authors - Zhao Y Y
  • KD Validated
mouseWB

Hum Mol Genet

Abnormalities of motor function, transcription and cerebellar structure in mouse models of THAP1 dystonia.

Authors - Marta Ruiz
  • KO Validated
humanWB

Nucleic Acids Res

Stress-induced changes in gene interactions in human cells.

Authors - Nayak Renuka R RR
mouseWB

Dev Cell

The DYT6 Dystonia Protein THAP1 Regulates Myelination within the Oligodendrocyte Lineage.

Authors - Dhananjay Yellajoshyula
mouseWB

Ann Neurol

Direct interaction between causative genes of DYT1 and DYT6 primary dystonia.

Authors - Gavarini Sophie S