Thrombomodulin Polyclonal antibody

Thrombomodulin Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 14318-1-AP

Synonyms

CD141, Fetomodulin, THBD, THRM, thrombomodulin, TM



Tested Applications

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14318-1-AP targets Thrombomodulin in WB, IHC, IF, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Thrombomodulin fusion protein Ag5558
Full Name thrombomodulin
Calculated molecular weight 60 kDa
Observed molecular weight 105 kDa
GenBank accession numberBC035602
Gene symbol THBD
Gene ID (NCBI) 7056
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).

Protocols

Product Specific Protocols
WB protocol for Thrombomodulin antibody 14318-1-APDownload protocol
IHC protocol for Thrombomodulin antibody 14318-1-APDownload protocol
FC protocol for Thrombomodulin antibody 14318-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Clin Appl Thromb Hemost

Endocardial Changes in Nonvalvular Atrial Fibrillation Without Atrial Thrombus-Thrombomodulin and Tissue Factor Pathway Inhibitor.

Authors - Kang An
humanWB

Biochim Biophys Acta

The histone methyltransferase SETD1A regulates thrombomodulin transcription in vascular endothelial cells.

Authors - Zilong Li
humanIHC

Res Sq

Urinary SARS-CoV-2 RNA is An Indicator For The Progression and Prognosis of COVID-19 Disease.

Authors - Maoqing Tian
humanWB,IF

Ther Apher Dial

Decreased Expression of Thrombomodulin in Endothelial Cells by Fibroblast Growth Factor-23/α-Klotho.

Authors - Kenji Tanaka
humanWB

Front Cell Dev Biol

Crocin Improves Endothelial Mitochondrial Dysfunction via GPx1/ROS/KCa3.1 Signal Axis in Diabetes.

Authors - Xuemei Li