Thrombomodulin Polyclonal antibody

Thrombomodulin Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)





Cat no : 14318-1-AP


CD141, Fetomodulin, THBD, THRM, thrombomodulin, TM

Tested Applications

Positive WB detected inHUVEC cells, human heart tissue, THP-1 cells
Positive FC detected inA431 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14318-1-AP targets Thrombomodulin in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Thrombomodulin fusion protein Ag5558
Full Name thrombomodulin
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberBC035602
Gene Symbol THBD
Gene ID (NCBI) 7056
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).


Product Specific Protocols
WB protocol for Thrombomodulin antibody 14318-1-APDownload protocol
IHC protocol for Thrombomodulin antibody 14318-1-APDownload protocol
FC protocol for Thrombomodulin antibody 14318-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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