THP-1 nuclear extract

Synonyms

THP-1, THP1, THP 1, nuclear extract, nuclear, extract, lysate, nuclear lysate, 36076, monocytic, monocyte, leukemia, monocytic leukemia, acute monocytic leukemia, human, monocyte-derived, monocyte-derived macrophages, macrophage, macrophages, phagocyte, phagocytic, phagocytes, phagocytosis, DMSO, PMA, phorbol, phorbol ester, esters, ester, vitamin D3, D3, atherosclerosis, plaque, plaque formation, atheroschlerotic, immunoglobulin, Ig, foam cell, foam cells, foam cell formation, Fc, C3b, IL-1, interleukin, lysosyme, alpha-napthyl butyrate esterase, esterase, monocyte differentiation, differentiation, TPA

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Contents

2 x 100 µg of THP-1 nuclear extract at 2.5 µg/µl.

Background

THP-1 nuclear extract was prepared from a cell culture of the human acute monocytic leukemia THP-1 cell line. The THP-1 cell line was originally derived from the peripheral blood of a 1 year old boy with acute monocytic leukemia. THP-1 cells share similar properties to human monocyte-derived macrophages. These cells differentiate into mature macrophages (phagocytes) when grown in the presence of DMSO or phorbol 12-myristate 13-acetate (TPA or PMA). When stimulated with DMSO or vitamin D3, THP-1 cells adhere to the walls of cell culture flasks, and are therefore frequently utilized as in vitro model systems to study foam cell formation in relation to atherosclerosis. The THP-1 cell line is characterized by the expression of Fc and C3b receptors, the production of IL-1, and the lack of expression of cytoplasmic and surface immunoglobulins. In addition, these cells are phagocytic, produce lysosymes and stain positive for alpha-napthyl butyrate esterase. Because of these properties, they are often used in research to study monocyte differentiation.

Application Notes

THP-1 nuclear extract is recommended for use in studies related to 1) blood monocytes, 2) monocyte differentiation and 3) atherosclerosis.

Extract Origin

Human monocytes

Extract Composition

THP-1 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl₂, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

Quality Control

Extracts have been quality control tested by Western blot and TransAM^® Transcription Factor ELISAs.