TIGAR Recombinant antibody, PBS Only

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  WB analysis using 85052-4-RR (same clone as 85052-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85052-4-RR (TIGAR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85052-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85052-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85052-4-RR against Human TIGAR were performed. The affinity constant is 0.308 nM.

TIGAR Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 85052-4-PBS

Clone No.242625E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

C12orf5, 242625E7, EC:3.1.3.46, Fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator

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Product Information

85052-4-PBS targets TIGAR in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TIGAR fusion protein Ag17532
• Full Name: chromosome 12 open reading frame 5
• Calculated Molecular Weight: 270 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC012340
• Gene Symbol: TIGAR
• Gene ID (NCBI): 57103
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NQ88
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The TP53-induced glycolysis and apoptosis regulator (TIGAR), first reported in 2006, is a downstream target gene of p53 and an important factor involved in glycolysis and apoptosis . It is indispensable in metabolism and is involved in metabolic syndrome, including hyperglycemia, insulin resistance, alcoholic fatty liver and tissue ischemia. TIGAR is highly expressed in many cancer cells to promote cell survival.