TIMM13 Polyclonal antibody

TIMM13 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 11973-1-AP


ppv1, TIM13, TIM13B, TIMM13, TIMM13A, TIMM13B

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11973-1-AP targets TIMM13 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIMM13 fusion protein Ag2596
Full Name translocase of inner mitochondrial membrane 13 homolog (yeast)
Calculated Molecular Weight 95 aa, 11 kDa
Observed Molecular Weight 11 kDa
GenBank Accession NumberBC008607
Gene Symbol TIMM13
Gene ID (NCBI) 26517
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TIMM13 gene, also known as TIM13, TIM13B, TIM M13A or TIMM13B, encodes mitochondrial import inner membrane translocase subunit Tim13 belonging to the small Tim family. TIM13 functions as mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Proteins of the intermembrane space (IMS) of mitochondria are typically synthesized without presequences. TIM13 contains four conserved cysteine residues that bind a zinc ion as cofactor. Import of TIM13 did not depend on the membrane potential or ATP hydrolysis. Upon import into mitochondria TIM13 adopted a stably folded conformation in the IMS.


Product Specific Protocols
WB protocol for TIMM13 antibody 11973-1-APDownload protocol
IHC protocol for TIMM13 antibody 11973-1-APDownload protocol
IF protocol for TIMM13 antibody 11973-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Stem Cell

Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.

Authors - Rashim Pal Singh

Am J Hum Genet

The mitochondrial disulfide relay system protein GFER is mutated in autosomal-recessive myopathy with cataract and combined respiratory-chain deficiency.

Authors - Di Fonzo Alessio A


Function of hTim8a in complex IV assembly in neuronal cells provides insight into pathomechanism underlying Mohr-Tranebjærg syndrome.

Authors - Yilin Kang


TIM29 is a subunit of the human carrier translocase required for protein transport.

Authors - Sylvie Callegari

Mol Genet Genomic Med

Functional analysis of a novel mutation in the TIMM8A gene that causes deafness-dystonia-optic neuronopathy syndrome.

Authors - Addison Neighbors


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Süleyman (Verified Customer) (01-22-2022)

I tested for IMS labelling of mitochondria. I used 1:700 Alexa Fluor 488 secondary antibody. Fixed 4% paraformaldehyde in PBS for 10 minutes at RT and 0.1% Triton X permeabilization. Overnight primary ab labelling at 4 oC. 1 hour secondary ab labelling at RT.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: HeLa FlipIn
TIMM13 Antibody Immunofluorescence validation (1:100 dilution) in HeLa FlipIn (Cat no:11973-1-AP)