|Positive WB detected in||HeLa cells, HepG 2 cells|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11973-1-AP targets TIMM13 in WB, IHC, IF,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||TIMM13 fusion protein Ag2596|
|Full Name||translocase of inner mitochondrial membrane 13 homolog (yeast)|
|Calculated molecular weight||95 aa, 11 kDa|
|Observed molecular weight||11 kDa|
|GenBank accession number||BC008607|
|Gene ID (NCBI)||26517|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TIMM13 gene, also known as TIM13, TIM13B, TIM M13A or TIMM13B, encodes mitochondrial import inner membrane translocase subunit Tim13 belonging to the small Tim family. TIM13 functions as mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Proteins of the intermembrane space (IMS) of mitochondria are typically synthesized without presequences. TIM13 contains four conserved cysteine residues that bind a zinc ion as cofactor. Import of TIM13 did not depend on the membrane potential or ATP hydrolysis. Upon import into mitochondria TIM13 adopted a stably folded conformation in the IMS.
Mol Genet Genomic Med
Functional analysis of a novel mutation in the TIMM8A gene that causes deafness-dystonia-optic neuronopathy syndrome.
Cell Stem Cell
Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.
TIM29 is a subunit of the human carrier translocase required for protein transport.
Function of hTim8a in complex IV assembly in neuronal cells provides insight into pathomechanism underlying Mohr-Tranebjærg syndrome.
Am J Hum Genet
The mitochondrial disulfide relay system protein GFER is mutated in autosomal-recessive myopathy with cataract and combined respiratory-chain deficiency.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Süleyman (Verified Customer) (01-22-2022)
I tested for IMS labelling of mitochondria. I used 1:700 Alexa Fluor 488 secondary antibody. Fixed 4% paraformaldehyde in PBS for 10 minutes at RT and 0.1% Triton X permeabilization. Overnight primary ab labelling at 4 oC. 1 hour secondary ab labelling at RT.