TIMP1 Polyclonal antibody

TIMP1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

53

Cat no : 16644-1-AP

Synonyms

CLGI, Collagenase inhibitor, EPA, EPO, HCI, Metalloproteinase inhibitor 1, TIMP, TIMP 1, TIMP1, TIMP-1

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Validation Data Gallery

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  WB analysis using 16644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16644-1-AP (TIMP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16644-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16644-1-AP (TIMP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16644-1-AP (TIMP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HT-29 using 16644-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using TIMP1 antibody (16644-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HL-60 cells, HT-29 cells, HeLa cells
• Positive IF detected in: HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 45 publications below
• IHC: See 13 publications below
• IF: See 1 publications below

Product Information

The immunogen of 16644-1-AP is TIMP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, bovine, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIMP1 fusion protein Ag10006
• Full Name: TIMP metallopeptidase inhibitor 1
• Calculated molecular weight: 23 kDa
• Observed molecular weight: 23 kDa
• GenBank accession number: BC000866
• Gene symbol: TIMP1
• Gene ID (NCBI): 7076
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TIMP1 is a member of the family of matrix metalloproteinase inhibitors, which contains four members (TIMP1, TIMP2, TIMP3, and TIMP4). Tissue inhibitors of metalloproteinases (TIMPs) are multifaceted molecules that exhibit properties beyond their classical proteinase inhibitory function. TIMP1 has several MMP-independent functions such as modulation of angiogenesis, promotion of cell proliferation, and inhibition of apoptosis. TIMP1 plays important role in cell cycle regulation and cancer progression. Recently, clinical studies have shown that the aberrant expression of TIMP1 is associated with an unfavorable prognosis in a series of tumors, such as gastric cancer, papillary thyroid carcinoma, cutaneous melanoma and breast cancer. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium.

Publications

Species	Application	Title
human	WB	Inflammation; Effects of IL-1β on MMP-9 Expression in Cementoblast-Derived Cell Line and MMP-Mediated Degradation of Type I Collagen.; Authors - Mingyuan Du
rat	WB	Phytomedicine; Kadsura heteroclita stem suppresses the onset and progression of adjuvant-induced arthritis in rats.; Authors - Huanghe Yu
rat	WB	Pain Physician; Toxicity Effects of Methylene Blue on Rat Intervertebral Disc Annulus Fibrosus Cells.; Authors - Liang Zhang
rat	WB	Toxicol Appl Pharmacol; Gambogic acid attenuates liver fibrosis by inhibiting the PI3K/AKT and MAPK signaling pathways via inhibiting HSP90.; Authors - Zhenlong Yu
mouse	WB	BMC Oral Health; ZnO nanoparticles inhibit the activity of Porphyromonas gingivalis and Actinomyces naeslundii and promote the mineralization of the cementum.; Authors - Jingyu Wang
rat	IHC	Evid Based Complement Alternat Med; A Compound of Chinese Herbs Protects against Alcoholic Liver Fibrosis in Rats via the TGF-β1/Smad Signaling Pathway.; Authors - Xiaomeng Li

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