TIMP1 Polyclonal antibody
TIMP1 Polyclonal Antibody for IF, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 16644-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, HT-29 cells, HeLa cells |
| Positive IF detected in | HT-29 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 37 publications below |
| IHC | See 10 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 16644-1-AP is TIMP1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | bovine, human, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TIMP1 fusion protein Ag10006 |
| Full Name | TIMP metallopeptidase inhibitor 1 |
| Calculated molecular weight | 23 kDa |
| Observed molecular weight | 23 kDa |
| GenBank accession number | BC000866 |
| Gene symbol | TIMP1 |
| Gene ID (NCBI) | 7076 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TIMP1 is a member of the family of matrix metalloproteinase inhibitors, which contains four members (TIMP1, TIMP2, TIMP3, and TIMP4). Tissue inhibitors of metalloproteinases (TIMPs) are multifaceted molecules that exhibit properties beyond their classical proteinase inhibitory function. TIMP1 has several MMP-independent functions such as modulation of angiogenesis, promotion of cell proliferation, and inhibition of apoptosis. TIMP1 plays important role in cell cycle regulation and cancer progression. Recently, clinical studies have shown that the aberrant expression of TIMP1 is associated with an unfavorable prognosis in a series of tumors, such as gastric cancer, papillary thyroid carcinoma, cutaneous melanoma and breast cancer. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TIMP1 antibody 16644-1-AP | Download protocol |
| IF protocol for TIMP1 antibody 16644-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Inflammation Effects of IL-1β on MMP-9 Expression in Cementoblast-Derived Cell Line and MMP-Mediated Degradation of Type I Collagen. | ||
Phytomedicine Kadsura heteroclita stem suppresses the onset and progression of adjuvant-induced arthritis in rats. | ||
Pain Physician Toxicity Effects of Methylene Blue on Rat Intervertebral Disc Annulus Fibrosus Cells. | ||
Toxicol Appl Pharmacol Gambogic acid attenuates liver fibrosis by inhibiting the PI3K/AKT and MAPK signaling pathways via inhibiting HSP90. | ||
BMC Oral Health ZnO nanoparticles inhibit the activity of Porphyromonas gingivalis and Actinomyces naeslundii and promote the mineralization of the cementum. | ||
Evid Based Complement Alternat Med A Compound of Chinese Herbs Protects against Alcoholic Liver Fibrosis in Rats via the TGF-β1/Smad Signaling Pathway. |
