TIMP1 Polyclonal antibody

TIMP1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16644-1-AP

Synonyms

CLGI, Collagenase inhibitor, EPA, EPO, HCI, Metalloproteinase inhibitor 1, TIMP, TIMP 1, TIMP1, TIMP-1



Tested Applications

Positive WB detected inHL-60 cells, HT-29 cells, HeLa cells
Positive IF detected inHT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 16644-1-AP is TIMP1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity bovine, human, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIMP1 fusion protein Ag10006
Full Name TIMP metallopeptidase inhibitor 1
Calculated molecular weight 23 kDa
Observed molecular weight23 kDa
GenBank accession numberBC000866
Gene symbol TIMP1
Gene ID (NCBI) 7076
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TIMP1 is a member of the family of matrix metalloproteinase inhibitors, which contains four members (TIMP1, TIMP2, TIMP3, and TIMP4). Tissue inhibitors of metalloproteinases (TIMPs) are multifaceted molecules that exhibit properties beyond their classical proteinase inhibitory function. TIMP1 has several MMP-independent functions such as modulation of angiogenesis, promotion of cell proliferation, and inhibition of apoptosis. TIMP1 plays important role in cell cycle regulation and cancer progression. Recently, clinical studies have shown that the aberrant expression of TIMP1 is associated with an unfavorable prognosis in a series of tumors, such as gastric cancer, papillary thyroid carcinoma, cutaneous melanoma and breast cancer. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium.

Protocols

Product Specific Protocols
WB protocol for TIMP1 antibody 16644-1-APDownload protocol
IF protocol for TIMP1 antibody 16644-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Inflammation

Effects of IL-1β on MMP-9 Expression in Cementoblast-Derived Cell Line and MMP-Mediated Degradation of Type I Collagen.

Authors - Mingyuan Du
ratWB

Phytomedicine

Kadsura heteroclita stem suppresses the onset and progression of adjuvant-induced arthritis in rats.

Authors - Huanghe Yu
ratWB

Pain Physician

Toxicity Effects of Methylene Blue on Rat Intervertebral Disc Annulus Fibrosus Cells.

Authors - Liang Zhang
ratWB

Toxicol Appl Pharmacol

Gambogic acid attenuates liver fibrosis by inhibiting the PI3K/AKT and MAPK signaling pathways via inhibiting HSP90.

Authors - Zhenlong Yu
mouseWB

BMC Oral Health

ZnO nanoparticles inhibit the activity of Porphyromonas gingivalis and Actinomyces naeslundii and promote the mineralization of the cementum.

Authors - Jingyu Wang
ratIHC

Evid Based Complement Alternat Med

A Compound of Chinese Herbs Protects against Alcoholic Liver Fibrosis in Rats via the TGF-β1/Smad Signaling Pathway.

Authors - Xiaomeng Li