TIMP1 Polyclonal antibody

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  WB analysis using 16644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16644-1-AP (TIMP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16644-1-AP (TIMP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16644-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16644-1-AP (TIMP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HT-29 using 16644-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using TIMP1 antibody (16644-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

TIMP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 16644-1-AP

Publications(96)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TIMP-1, CLGI, Collagenase inhibitor, EPA, Erythroid-potentiating activity

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Tested Applications

• Positive WB detected in: HL-60 cells, HT-29 cells, HeLa cells, SKOV-3 cells
• Positive IF/ICC detected in: HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 78 publications below
• IHC: See 24 publications below
• IF: See 7 publications below

Product Information

16644-1-AP targets TIMP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rabbit, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIMP1 fusion protein Ag10006
• Full Name: TIMP metallopeptidase inhibitor 1
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC000866
• Gene Symbol: TIMP1
• Gene ID (NCBI): 7076
• RRID: AB_2878292
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P01033
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TIMP1 is a member of the family of matrix metalloproteinase inhibitors, which contains four members (TIMP1, TIMP2, TIMP3, and TIMP4). Tissue inhibitors of metalloproteinases (TIMPs) are multifaceted molecules that exhibit properties beyond their classical proteinase inhibitory function. TIMP1 has several MMP-independent functions such as modulation of angiogenesis, promotion of cell proliferation, and inhibition of apoptosis. TIMP1 plays important role in cell cycle regulation and cancer progression. Recently, clinical studies have shown that the aberrant expression of TIMP1 is associated with an unfavorable prognosis in a series of tumors, such as gastric cancer, papillary thyroid carcinoma, cutaneous melanoma and breast cancer. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium.

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.; Authors - Lei Shi
human	WB,IF	J Neuroinflammation; Identification of TIMP1 as an inflammatory biomarker associated with temporal lobe epilepsy based on integrated bioinformatics and experimental analyses; Authors - Ya He
human	WB	Cell Death Dis; α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation; Authors - Hao Wang
human	WB	Biomed Pharmacother; TrxR/Trx inhibitor butaselen ameliorates pulmonary fibrosis by suppressing NF-κB/TGF-β1/Smads signaling; Authors - Yifan Chen
human	IF	Cell Mol Gastroenterol Hepatol; Sestrin Proteins Protect Against Lipotoxicity-Induced Oxidative Stress in the Liver via Suppression of C-Jun N-Terminal Kinases.; Authors - Zhigang Fang
	IHC	Mater Sci Eng C Mater Biol Appl; Injectable recombinant human collagen-derived material with high cell adhesion activity limits adverse remodelling and improves pelvic floor function in pelvic floor dysfunction rats.; Authors - Hu Li