TIMP1 Polyclonal antibody

TIMP1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

39

Cat no : 16644-1-AP

Synonyms

CLGI, Collagenase inhibitor, EPA, EPO, HCI, Metalloproteinase inhibitor 1, TIMP, TIMP 1, TIMP1

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Validation Data Gallery

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  WB analysis using 16644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16644-1-AP (TIMP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 16644-1-AP

  Immunofluorescent analysis of HepG2 cells using 16644-1-AP (TIMP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HL-60 cells, HT-29 cells, HeLa cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 34 publications below
• IHC: See 8 publications below
• IF: See 1 publications below

Product Information

The immunogen of 16644-1-AP is TIMP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: bovine, human, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIMP1 fusion protein Ag10006
• Full Name: TIMP metallopeptidase inhibitor 1
• Calculated molecular weight: 23 kDa
• Observed molecular weight: 23 kDa
• GenBank accession number: BC000866
• Gene symbol: TIMP1
• Gene ID (NCBI): 7076
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	WB	Inflammation; Effects of IL-1β on MMP-9 Expression in Cementoblast-Derived Cell Line and MMP-Mediated Degradation of Type I Collagen.; Authors - Mingyuan Du
rat	WB	Phytomedicine; Kadsura heteroclita stem suppresses the onset and progression of adjuvant-induced arthritis in rats.; Authors - Huanghe Yu
rat	WB	Pain Physician; Toxicity Effects of Methylene Blue on Rat Intervertebral Disc Annulus Fibrosus Cells.; Authors - Liang Zhang
rat	WB	Toxicol Appl Pharmacol; Gambogic acid attenuates liver fibrosis by inhibiting the PI3K/AKT and MAPK signaling pathways via inhibiting HSP90.; Authors - Zhenlong Yu
mouse	WB	BMC Oral Health; ZnO nanoparticles inhibit the activity of Porphyromonas gingivalis and Actinomyces naeslundii and promote the mineralization of the cementum.; Authors - Jingyu Wang
rat	IHC	Evid Based Complement Alternat Med; A Compound of Chinese Herbs Protects against Alcoholic Liver Fibrosis in Rats via the TGF-β1/Smad Signaling Pathway.; Authors - Xiaomeng Li

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