TIMP-2 Polyclonal antibody

TIMP-2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17353-1-AP

Synonyms

CSC 21K, Metalloproteinase inhibitor 2, TIMP 2, TIMP2, TIMP-2



Tested Applications

Positive IHC detected inhuman kidney tissue, mouse lung tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

17353-1-AP targets TIMP-2 in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIMP-2 fusion protein Ag10051
Full Name TIMP metallopeptidase inhibitor 2
Calculated molecular weight 220 aa, 24 kDa
GenBank accession numberBC052605
Gene symbol TIMP2
Gene ID (NCBI) 7077
RRIDAB_2287495
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The TIMPs are natural highly specific endogenous inhibitors of MMPs. Human TIMP family consists of four 21-28 kDa proteins known as TIMP1, TIMP2, TIMP3, and TIMP4 encoded by four paralogous genes.TIMPs are vital to the maintenance of ECM homeostasis primarily through their MMP inhibitory functions. TIMP2, a member of the TIMP family, regulates the proteolytic activity of all MMPs and is involved in cell differentiation, growth, migration, angiogenesis, and apoptosis. Urinary TIMP2 has been recently recognized as an early biomarker to predict Acute kidney injury (AKI) in critically ill patients.

Protocols

Product Specific Protocols
IHC protocol for TIMP-2 antibody 17353-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Clin Cancer Res

RAB37 Hypermethylation Regulates Metastasis and Resistance to Docetaxel-Based Induction Chemotherapy in Nasopharyngeal Carcinoma.

Authors - Yingqin Li
human

Cell Death Dis

α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation

Authors - Hao Wang
mouse

Int J Mol Sci

PTEN Inhibitor Treatment Lowers Muscle Plasma Membrane Damage and Enhances Muscle ECM Homeostasis after High-Intensity Eccentric Exercise in Mice

Authors - Baile Wu
human

Int J Biol Sci

TDG suppresses the migration and invasion of human colon cancer cells via the DNMT3A/TIMP2 axis.

Authors - Jiyu Miao
human

Mar Drugs

11-epi-Sinulariolide Acetate Reduces Cell Migration and Invasion of Human Hepatocellular Carcinoma by Reducing the Activation of ERK1/2, p38MAPK and FAK/PI3K/AKT/mTOR Signaling Pathways.

Authors - Jen-Jie Lin
humanIHC

Cell Signal

Integration of exosomal miR-106a and mesothelial cells facilitates gastric cancer peritoneal dissemination.

Authors - Meng Zhu