TIMP-2 Polyclonal antibody

TIMP-2 Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

30

Cat no : 17353-1-AP

Synonyms

CSC 21K, Metalloproteinase inhibitor 2, TIMP 2, TIMP2, TIMP-2

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Validation Data Gallery

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  IHC staining of human kidney using 17353-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17353-1-AP (TIMP-2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 17353-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17353-1-AP (TIMP-2 antibody at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 27 publications below
• IHC: See 7 publications below
• IF: See 1 publications below

Product Information

17353-1-AP targets TIMP-2 in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIMP-2 fusion protein Ag10051
• Full Name: TIMP metallopeptidase inhibitor 2
• Calculated molecular weight: 220 aa, 24 kDa
• GenBank accession number: BC052605
• Gene symbol: TIMP2
• Gene ID (NCBI): 7077
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The TIMPs are natural highly specific endogenous inhibitors of MMPs. Human TIMP family consists of four 21-28 kDa proteins known as TIMP1, TIMP2, TIMP3, and TIMP4 encoded by four paralogous genes.TIMPs are vital to the maintenance of ECM homeostasis primarily through their MMP inhibitory functions. TIMP2, a member of the TIMP family, regulates the proteolytic activity of all MMPs and is involved in cell differentiation, growth, migration, angiogenesis, and apoptosis. Urinary TIMP2 has been recently recognized as an early biomarker to predict Acute kidney injury (AKI) in critically ill patients.

Publications

Species	Application	Title
human	WB,IHC,IF	Clin Cancer Res; RAB37 Hypermethylation Regulates Metastasis and Resistance to Docetaxel-Based Induction Chemotherapy in Nasopharyngeal Carcinoma.; Authors - Yingqin Li
human	WB	Int J Biol Sci; TDG suppresses the migration and invasion of human colon cancer cells via the DNMT3A/TIMP2 axis.; Authors - Jiyu Miao
human	WB	Mar Drugs; 11-epi-Sinulariolide Acetate Reduces Cell Migration and Invasion of Human Hepatocellular Carcinoma by Reducing the Activation of ERK1/2, p38MAPK and FAK/PI3K/AKT/mTOR Signaling Pathways.; Authors - Jen-Jie Lin
human	WB,IHC	Cell Signal; Integration of exosomal miR-106a and mesothelial cells facilitates gastric cancer peritoneal dissemination.; Authors - Meng Zhu
human	WB,IHC	Int Immunopharmacol; Cryptotanshinone inhibits the growth and invasion of colon cancer by suppressing inflammation and tumor angiogenesis through modulating MMP/TIMP system, PI3K/Akt/mTOR signaling and HIF-1α nuclear translocation.; Authors - Lin Zhang
human	WB	Cell Tissue Res; PMID: 26022336; Authors - Yunpeng Xie