|Positive IHC detected in||human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11284-1-AP targets TLE1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TLE1 fusion protein Ag1801|
|Full Name||transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)|
|Calculated molecular weight||83 kDa|
|GenBank accession number||BC010100|
|Gene ID (NCBI)||7088|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Transducin-like enhancer protein 1(TLE1) is a transcriptional corepressor. It can bind to and regress expression of many transcription factors such as NFkB, which is mediated by FOXA2, CTNNB1 and TCF family member in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Expression of TLE1 is highest in skeletal muscle and liver, moderate in placenta, heart, kidney, and spleen, and weak in all other tissues including brain.