• Featured Product
  • KD/KO Validated

TLE3 Polyclonal antibody

TLE3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ChIP

Conjugate

Unconjugated

Cat no : 11372-1-AP

Synonyms

ESG, ESG3, GRG3, HsT18976, KIAA1547, TLE3



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human lung tissue, A431 cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11372-1-AP targets TLE3 in WB, IP, IHC, IF, CoIP, ChIP applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TLE3 fusion protein Ag1930
Full Name transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
Calculated molecular weight 772 aa, 83 kDa
Observed molecular weight 83 kDa
GenBank accession numberBC015729
Gene symbol TLE3
Gene ID (NCBI) 7090
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane

Protocols

Product Specific Protocols
WB protocol for TLE3 antibody 11372-1-APDownload protocol
IHC protocol for TLE3 antibody 11372-1-APDownload protocol
IF protocol for TLE3 antibody 11372-1-APDownload protocol
IP protocol for TLE3 antibody 11372-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Oncotarget

Transducin-like enhancer of split 3 regulates proliferation of melanoma cells via histone deacetylase activity.

Authors - Masahiro Ogawa
  • KD Validated
mouseWB

Cell Rep

PARP1-mediated PARylation activity is essential for oligodendroglial differentiation and CNS myelination.

Authors - Yan Wang
WB

Am J Physiol Endocrinol Metab

Pharmacological modulation of RORα controls the fat browning, adaptive thermogenesis and body weight in mice.

Authors - Martine Auclair
humanWB

Acta Pharmacol Sin

RNF6 promotes myeloma cell proliferation and survival by inducing glucocorticoid receptor polyubiquitination.

Authors - Ying Ren
humanWB,IF

J Biol Chem

The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor.

Authors - Shoichiro Kokabu
  • KD Validated
humanWB

J Mol Med (Berl)

Transducin-like enhancer of split 3 (TLE3) in adipose tissue is increased in situations characterized by decreased PPARγ gene expression.

Authors - Francisco José Ortega

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tongbin (Verified Customer) (08-05-2020)

I did western blots using mouse heart samples. I observed a TLE3 band with correct molecular weight of about 80kD.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart tissue