|Positive WB detected in||HeLa cells, HepG2 cells, human lung tissue, A431 cells, HEK-293 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11372-1-AP targets TLE3 in WB, IP, IHC, IF, CoIP, ChIP applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TLE3 fusion protein Ag1930|
|Full Name||transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)|
|Calculated molecular weight||772 aa, 83 kDa|
|Observed molecular weight||83 kDa|
|GenBank accession number||BC015729|
|Gene ID (NCBI)||7090|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane
|Product Specific Protocols|
|WB protocol for TLE3 antibody 11372-1-AP||Download protocol|
|IHC protocol for TLE3 antibody 11372-1-AP||Download protocol|
|IF protocol for TLE3 antibody 11372-1-AP||Download protocol|
|IP protocol for TLE3 antibody 11372-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Transducin-like enhancer of split 3 regulates proliferation of melanoma cells via histone deacetylase activity.
PARP1-mediated PARylation activity is essential for oligodendroglial differentiation and CNS myelination.
Am J Physiol Endocrinol Metab
Pharmacological modulation of RORα controls the fat browning, adaptive thermogenesis and body weight in mice.
Acta Pharmacol Sin
RNF6 promotes myeloma cell proliferation and survival by inducing glucocorticoid receptor polyubiquitination.
J Biol Chem
The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor.
J Mol Med (Berl)
Transducin-like enhancer of split 3 (TLE3) in adipose tissue is increased in situations characterized by decreased PPARγ gene expression.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tongbin (Verified Customer) (08-05-2020)
I did western blots using mouse heart samples. I observed a TLE3 band with correct molecular weight of about 80kD.