Name: TLE3 Rabbit Polyclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IP, IHC, IF, chIP, ELISA
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Images See all

TLE3 Antibody WB HEK-293 cells 11372-1-AP

WB result of TLE3 antibody (11372-1-AP, 1:500) with si-Control and si-TLE3 transfected HEK293 cells.

HepG2 cells were subjected to SDS PAGE followed by western blot with 11372-1-AP(TLE3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

TLE3 Antibody IHC human breast cancer tissue 11372-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer slide using 11372-1-AP( TLE3 Antibody) at dilution of 1:50

TLE3 Antibody IHC human lung cancer tissue 11372-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer slide using 11372-1-AP( TLE3 Antibody) at dilution of 1:50

IP Result of anti-TLE3 (IP:11372-1-AP, 4ug; Detection:11372-1-AP 1:300) with HeLa cells lysate 1600ug.

Immunofluorescent analysis of HepG2 cells using 11372-1-AP(TLE3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Species specificity:

human, mouse, rat

Positive WB detected in:

HepG2 cells, HEK-293

Positive IP detected in:

HeLa cells

Positive IHC detected in:

human breast cancer tissue, human lung cancer tissue

Positive IF detected in:

HepG2 cells

Recommended dilution:

WB : 1:500-1:2400

IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB

IHC : 1:20-1:200

IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

TLE3 fusion protein Ag1930

Full name:

transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)

Calculated molecular weight:

772aa,83 kDa

Observed molecular weight:

83 kDa

GenBank accession number:

BC015729

Gene ID (NCBI):

7090

Gene symbol

TLE3

Synonyms

ESG, ESG3, GRG3, HsT18976, KIAA1547, TLE3

Background

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane

Showing 1- [6] of [6] Publications

Author:		Shoichiro Kokabu
Pubmed ID:		28607151
Journal:		J Biol Chem
Title:		The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor.
Application:		WB,IF
KD validated
Species:		human

Author:		Shaojun Xing
Pubmed ID:		30045946
Journal:		J Exp Med
Title:		Tle corepressors are differentially partitioned to instruct CD8+ T cell lineage choice and identity.
Application:		chIP
KO validated
Species:		mouse

Author:		Villanueva Claudio J CJ
Pubmed ID:		23473036
Journal:		Cell Metab
Title:		Adipose subtype-selective recruitment of TLE3 or Prdm16 by PPARγ specifies lipid storage versus thermogenic gene programs.
Application:		WB
KO validated
Species:		mouse

Author:		Francisco José Ortega
Pubmed ID:		25249007
Journal:		J Mol Med (Berl)
Title:		Transducin-like enhancer of split 3 (TLE3) in adipose tissue is increased in situations characterized by decreased PPARγ gene expression.
Application:		WB

Species:		human

Author:		Villanueva Claudio J CJ
Pubmed ID:		21459326
Journal:		Cell Metab
Title:		TLE3 is a dual-function transcriptional coregulator of adipogenesis.
Application:		WB,ChIP

Species:		mouse

Author:		Naderi Ali A
Pubmed ID:		22577344
Journal:		Neoplasia
Title:		Cross-regulation between FOXA1 and ErbB2 signaling in estrogen receptor-negative breast cancer.
Application:		ChIP

Species:		human

WB Figures

A431 cells were subjected to SDS PAGE followed by western blot with 11372-1-AP(TLE3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours

WB result of TLE3 antibody (11372-1-AP, 1:500) with si-Control and si-TLE3 transfected HEK293 cells.

HeLa cells were subjected to SDS PAGE followed by western blot with 11372-1-AP(TLE3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 11372-1-AP(TLE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 11372-1-AP(TLE3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

HepG2 cells were subjected to SDS PAGE followed by western blot with 11372-1-AP(TLE3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

HepG2 cells were subjected to SDS PAGE followed by western blot with 11372-1-AP(TLE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

TLE3 Antibody WB human lung tissue 11372-1-AP

human lung tissue were subjected to SDS PAGE followed by western blot with 11372-1-AP(TLE3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemistry of paraffin-embedded human breast cancer slide using 11372-1-AP( TLE3 Antibody) at dilution of 1:50

Immunohistochemistry of paraffin-embedded human lung cancer slide using 11372-1-AP( TLE3 Antibody) at dilution of 1:50

IP Figures

IP Result of anti-TLE3 (IP:11372-1-AP, 4ug; Detection:11372-1-AP 1:300) with HeLa cells lysate 1600ug.

IF Figures

Immunofluorescent analysis of HepG2 cells using 11372-1-AP(TLE3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

TLE3 Antibody 6 Publications

Rabbit Polyclonal| Catalog number: 11372-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background

Showing 1- [6] of [6] Publications

WB Figures

IHC Figures

IP Figures

IF Figures

TLE3 Antibody 6 Publications

Rabbit Polyclonal| Catalog number: 11372-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background