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TLE3 Antibody 6 Publications

Rabbit Polyclonal| Catalog number: 11372-1-AP

Featured Product KD/KO validated

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Con: 34 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, chIP, ELISA
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Images See all

TLE3 Antibody WB HEK-293 cells 11372-1-AP

WB result of TLE3 antibody (11372-1-AP, 1:500) with si-Control and si-TLE3 transfected HEK293 cells.

TLE3 Antibody IHC human breast cancer tissue 11372-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer slide using 11372-1-AP( TLE3 Antibody) at dilution of 1:50

TLE3 Antibody IHC human lung cancer tissue 11372-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer slide using 11372-1-AP( TLE3 Antibody) at dilution of 1:50

TLE3 Antibody WB HepG2 cells 11372-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 11372-1-AP( TLE3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

TLE3 Antibody IP HeLa cells 11372-1-AP

IP Result of anti-TLE3 (IP:11372-1-AP, 4ug; Detection:11372-1-AP 1:300) with HeLa cells lysate 1600ug.

TLE3 Antibody IF HepG2 cells 11372-1-AP

Immunofluorescent analysis of HepG2 cells using 11372-1-AP(TLE3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Species specificity:
human, mouse, rat
Positive WB detected in:
HepG2 cells, A431 cells, HEK-293 cells, HeLa cells, human lung tissue
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human breast cancer tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:20-1:200
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
TLE3 fusion protein Ag1930
Full name:
transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
Calculated molecular weight:
772aa,83 kDa
Observed molecular weight:
83 kDa
GenBank accession number:
BC015729
Gene ID (NCBI):
7090
Gene symbol
TLE3
Synonyms
ESG, ESG3, GRG3, HsT18976, KIAA1547, TLE3

Background

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane


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