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TLE3 Polyclonal antibody

TLE3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 22094-1-AP

Synonyms

ESG, ESG3, GRG3, HsT18976, KIAA1547, TLE3



Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22094-1-AP is TLE3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TLE3 fusion protein Ag17300
Full Name transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
Calculated molecular weight 772 aa, 83 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC015729
Gene symbol TLE3
Gene ID (NCBI) 7090
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transducin-like enhancer of split 3(TLE3) is a corepressor that binds to a variant of transciption factor, which mediated by CTNNB1 and TCF family members in Wnt signaling. It's suggested that effect of full-length TLE family members be regulated by association with dominant-negative AES.

Protocols

Product Specific Protocols
WB protocol for TLE3 antibody 22094-1-APDownload protocol
IHC protocol for TLE3 antibody 22094-1-APDownload protocol
IF protocol for TLE3 antibody 22094-1-APDownload protocol
IP protocol for TLE3 antibody 22094-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Cells

Differential Regulation of TLE3 in Sertoli Cells of the Testes during Postnatal Development.

Authors - Sangho Lee
  • KD Validated