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TLR4 Antibody

TLR4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 19811-1-AP

Synonyms

ARMD10, CD284, hToll, TLR4, TOLL, toll like receptor 4



Tested Applications

Positive WB detected inmouse liver tissue, Daudi cells, rat liver tissue
Positive IHC detected inhuman placenta tissue, human liver cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

The 19811-1-AP immunogen is Fusion Protein TLR4 protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity calf, hamster, human, mouse, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TLR4 fusion protein Ag13841
Full Name toll-like receptor 4
Calculated molecular weight 839aa,96 kDa
Observed molecular weight 90-100 kDa
GenBank accession number BC117422
Gene symbol TLR4
Gene ID (NCBI) 7099
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described. The calculated molecular weights of the three TLR4 isoforms are 96, 91, and 73 kDa.

Protocols

Product Specific Protocols
WB protocol for TLR4 antibody 19811-1-APDownload protocol
IHC protocol for TLR4 antibody 19811-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Am J Transl Res

Muscone improves cardiac function in mice after myocardial infarction by alleviating cardiac macrophage-mediated chronic inflammation through inhibition of NF-κB and NLRP3 inflammasome.

Authors - Yingqiang Du
calfWB

J Appl Toxicol

Pharmacological inhibition of TLR4/NF-κB with TLR4-IN-C34 attenuated microcystin-leucine arginine toxicity in bovine Sertoli cells.

Authors - Elikanah Olusayo Adegoke
mouseWB

Stress

Regulating glycolysis, the TLR4 signal pathway and expression of RBM3 in mouse liver in response to acute cold exposure.

Authors - Hongzhao Shi
humanWB

Mediators Inflamm

Astragaloside IV Suppresses High Glucose-Induced NLRP3 Inflammasome Activation by Inhibiting TLR4/NF-κB and CaSR.

Authors - Bin Leng
  • KD Validated
mouseWB

J Immunol Res

Increased TLR4 Expression Aggravates Sepsis by Promoting IFN-γ Expression in CD38-/- Mice.

Authors - Qi Li
ratIHC,IF

Neurogastroenterol Motil

The role of toll-like receptor 4 and mast cell in the ameliorating effect of electroacupuncture on visceral hypersensitivity in rats.

Authors - Juan Yang