• Featured Product
  • KD/KO Validated

TLR4 Polyclonal antibody

TLR4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, Microtiter plate binding assay, IP, IHC, IF, CoIP, ChIP, Cell treatment, ELISA

Conjugate

Unconjugated

Cat no : 19811-1-AP

Synonyms

ARMD10, CD284, hToll, TLR4, TOLL, toll like receptor 4



Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman placenta tissue, mouse brain tissue, human liver cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

19811-1-AP targets TLR4 in WB, Microtiter plate binding assay, IP, IHC, IF, CoIP, ChIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit, hamster, pig, canine, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TLR4 fusion protein Ag13841
Full Name toll-like receptor 4
Calculated molecular weight 839 aa, 96 kDa
Observed molecular weight 90-110 kDa
GenBank accession numberBC117422
Gene symbol TLR4
Gene ID (NCBI) 7099
RRIDAB_10638446
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TLR4, also named CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP, and TRAF6, leading to NF-kB activation, cytokine secretion, and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described. The calculated molecular weights of the three TLR4 isoforms are 96, 91, and 73 kDa.

Protocols

Product Specific Protocols
WB protocol for TLR4 antibody 19811-1-APDownload protocol
IHC protocol for TLR4 antibody 19811-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Adv Mater

Biomimetic Immunosuppressive Exosomes that Inhibit Cytokine Storms Contribute to the Alleviation of Sepsis.

Authors - Yinan Li
humanELISA

J Clin Invest

Longistatin in tick saliva blocks advanced glycation end-product receptor activation.

Authors - Takeshi Anisuzzaman
humanWB

Gut Microbes

Fusobacterium nucleatum promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway

Authors - Jian-Wei Zhang
mouseIF,WB

Nat Commun

TLR4 signalling via Piezo1 engages and enhances the macrophage mediated host response during bacterial infection.

Authors - Jing Geng
mouseWB

Nat Commun

Alarmin-painted exosomes elicit persistent antitumor immunity in large established tumors in mice.

Authors - Bingfeng Zuo
  • KO Validated
mouseIP

Brain

Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2.

Authors - Yun Xia

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sara (Verified Customer) (10-25-2020)

B cells (mouse) were lysated in RIPA buffer with protein and phosphatase inhibitors. 10 µg of protein per lane were loaded in a 10% PAGE-SDS gel. Proteins were transferred to a PVFD membrane (Turbo Blot system) and incubated overnight with 1:1000 of anti-TLR4, followed by anti-rabbit-HRP (1:10.000) on the next day. Two bands of around 70 kDa are detected.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Primary B cells from mouse spleen
TLR4 Antibody Western Blot validation (1:1000 dilution) in Primary B cells from mouse spleen (Cat no:19811-1-AP)