TMEM132E Recombinant antibody

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  WB analysis using 82983-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82983-2-RR (TMEM132E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82983-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82983-2-RR against Human TMEM132E were performed. The affinity constant is 0.254 nM.

TMEM132E Recombinant Antibody for WB, ELISA

Cat No. 82983-2-RR

Clone No.230525E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

transmembrane protein 132E, 230525E12

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Tested Applications

• Positive WB detected in: MCF-7 cells, mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82983-2-RR targets TMEM132E in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TMEM132E fusion protein Ag26772
• Full Name: transmembrane protein 132E
• Calculated Molecular Weight: 116 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: NM_001304438
• Gene Symbol: TMEM132E
• Gene ID (NCBI): 124842
• RRID: AB_3670733
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6IEE7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transmembrane protein 132E (TMEM132E) is a member of the TMEM132 family which also include TMEM132A, TMEM132B, TMEM132C, TMEM132D (PMID: 29088312). The gene of TMEM132E is located within the DFNB99 locus on chromosome 17q12 and is highly expressed in the inner ear and other tissues including brain, kidney, lung, liver, spleen, heart, small intestine, colon, thymus, and stomach (PMID: 31656313). A variant of TMEM132E has been associated with autosomal-recessive nonsyndromic hearing loss DFNB99 (PMID: 25331638).