TMOD3 Polyclonal antibody

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  WB analysis of Jurkat using 12521-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12521-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12521-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 12521-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12521-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12521-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12521-1-AP

  IP result of anti-TMOD3 (IP:12521-1-AP, 4ug; Detection:12521-1-AP 1:700) with Jurkat cells lysate 4000 ug.

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  IHC staining of human kidney using 12521-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12521-1-AP (TMOD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 12521-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12521-1-AP (TMOD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 12521-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12521-1-AP (TMOD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMOD3 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12521-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Tropomodulin 3, Tropomodulin-3, UTMOD, U-Tmod

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Tested Applications

• Positive WB detected in: Jurkat cells, human heart tissue, human kidney tissue, human lung tissue, mouse brain tissue, mouse kidney tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human kidney tissue, human colon tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

12521-1-AP targets TMOD3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMOD3 fusion protein Ag3215
• Full Name: tropomodulin 3 (ubiquitous)
• Calculated Molecular Weight: 352 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC020542
• Gene Symbol: TMOD3
• Gene ID (NCBI): 29766
• RRID: AB_2205324
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NYL9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tropomodulin 3(TMOD3) belongs to the tropomodulin family, which controls cytoskeletal dynamics by regulating the end-capping of actin filaments. TMOD3 bridges the gap between structural integrity and signaling cascades. TMOD3 regulates cytoskeletal architecture and is implicated in cell adhesion, migration, and mechanotransduction.

Publications

Species	Application	Title
human	WB	Carcinogenesis; MiR-145 modulates the radiosensitivity of non-small cell lung cancer cells by suppression of TMOD3.; Authors - Hang Li
human	IHC	Comb Chem High Throughput Screen; Clinicopathological Characteristics, Prognosis, and Correlated Tumor Cell Function of Tropomodulin-3 in Pancreatic Adenocarcinoma; Authors - Bin Zhong
human	WB,IHC	Drug Resist Updat; TMOD3 accelerated resistance to immunotherapy in KRAS-mutated pancreatic cancer through promoting autophagy-dependent degradation of ASCL4; Authors - Zhiwei He