TNFR1/CD120a Polyclonal antibody

• 

  WB analysis of Raji using 21574-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21574-1-AP (TNFR1/CD120a Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21574-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21574-1-AP (TNFR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21574-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21574-1-AP (TNFR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 21574-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21574-1-AP (TNFR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 21574-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21574-1-AP (TNFR1/CD120a antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 21574-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21574-1-AP (TNFR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21574-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21574-1-AP (TNFR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21574-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21574-1-AP (TNFR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 21574-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TNFR1/CD120a antibody (21574-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 21574-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TNFR1 antibody (21574-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

TNFR1/CD120a Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 21574-1-AP

Publications(68)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Synonyms

TNFRSF1A, TBP1, p60, p55 R, p55

---

Tested Applications

• Positive WB detected in: Raji cells, HL-60 cells, human brain tissue, HeLa cells
• Positive IHC detected in: human brain tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 57 publications below
• IHC: See 9 publications below
• IF: See 16 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Product Information

21574-1-AP targets TNFR1/CD120a in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNFR1/CD120a fusion protein Ag16112
• Full Name: tumor necrosis factor receptor superfamily, member 1A
• Calculated Molecular Weight: 455 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC010140
• Gene Symbol: TNFR1
• Gene ID (NCBI): 7132
• RRID: AB_10734433
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P19438
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.

Publications

Species	Application	Title
	WB	Cancer Commun (Lond); Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies; Authors - Lu Tang
	WB,IF	Adv Healthc Mater; Hyaluronic Acid-based ROS-responsive Multifunctional Injectable Hydrogel Platform Accelerating Diabetic Wound Healing; Authors - Chen Shi
human	WB	Aging Dis; MiR-29a-3p Improves Acute Lung Injury by Reducing Alveolar Epithelial Cell PANoptosis.; Authors - Yanhui Cui
mouse	WB	PLoS Biol; A unique death pathway keeps RIPK1 D325A mutant mice in check at embryonic day 10.5.; Authors - Yingying Zhang
human	WB	Cell Death Discov; Repositioning linifanib as a potent anti-necroptosis agent for sepsis; Authors - Liang Yu
human	WB,IP	Cancer Lett; Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells.; Authors - Bei Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Xinda (Verified Customer) (08-16-2024)

It gave very nice and specific bands.

• Applications: Cell culture

FH

Tinne (Verified Customer) (08-14-2023)

This AB worked quite well - not the strongest signal - might be worth increasing the concentration. I got the best results when I did not permeabilise the cells.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Human hippocampal progenitor cells

TNFR1/CD120a Antibody Immunofluorescence validation (1:400 dilution) in Human hippocampal progenitor cells (Cat no:21574-1-AP)