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TNFR1 Antibody 12 Publications

Rabbit Polyclonal| Catalog number: 21574-1-AP

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Con: 40 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

TNFR1 Antibody WB Raji cells 21574-1-AP

Raji cells were subjected to SDS PAGE followed by western blot with 21574-1-AP( TNFR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

TNFR1 Antibody FC Raji cells 21574-1-AP

1X10^6 Raji cells were stained with 0.2ug TNFR1 antibody (21574-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

TNFR1 Antibody IHC human brain tissue 21574-1-AP

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21574-1-AP( TNFR1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TNFR1 Antibody IHC human brain tissue 21574-1-AP

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21574-1-AP( TNFR1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat
Positive WB detected in:
Raji cells, HeLa cells, HL-60 cells, human brain tissue
Positive IHC detected in:
human brain tissue, human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in:
Raji cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
TNFR1 fusion protein Ag16112
Full name:
tumor necrosis factor receptor superfamily, member 1A
Calculated molecular weight:
455aa,50 kDa
Observed molecular weight:
50 kDa
GenBank accession number:
BC010140
Gene ID (NCBI):
7132
Gene symbol
TNFRSF1A
Synonyms
CD120a, FPF, p55, p55 R, p60, TBP1, TBPI, TNF R, TNF R I, TNF R1, TNF R55, TNF Receptor 1, TNF Receptor I, TNF RI, TNFAR, TNFR I, TNFR1, TNFR55, TNFR60, TNFRSF1A

Background

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.


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