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TNFR1 Polyclonal antibody

TNFR1 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 21574-1-AP

Synonyms

CD120a, FPF, p55, p55 R, p60, TBP1, TBPI, TNF R, TNF R I, TNF R1, TNF R55, TNF Receptor 1, TNF Receptor I, TNF RI, TNFAR, TNFR I, TNFR1, TNFR55, TNFR60, TNFRSF1A



Tested Applications

Positive WB detected inRaji cells, HeLa cells, HL-60 cells, human brain tissue
Positive IHC detected inhuman brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21574-1-AP is TNFR1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNFR1 fusion protein Ag16112
Full Name tumor necrosis factor receptor superfamily, member 1A
Calculated molecular weight 455 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC010140
Gene symbol TNFRSF1A
Gene ID (NCBI) 7132
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.

Protocols

Product Specific Protocols
WB protocol for TNFR1 antibody 21574-1-APDownload protocol
IHC protocol for TNFR1 antibody 21574-1-APDownload protocol
FC protocol for TNFR1 antibody 21574-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Clin Invest

Membralin deficiency dysregulates astrocytic glutamate homeostasis leading to ALS-like impairment.

Authors - Lu-Lin Jiang
humanWB

J Exp Clin Cancer Res

KIAA1522 potentiates TNFα-NFκB signaling to antagonize platinum-based chemotherapy in lung adenocarcinoma.

Authors - Boshi Wang
ratWB

Evid Based Complement Alternat Med

The Herbal Medicine Scutellaria-Coptis Alleviates Intestinal Mucosal Barrier Damage in Diabetic Rats by Inhibiting Inflammation and Modulating the Gut Microbiota.

Authors - Boxun Zhang
humanWB

Food Chem Toxicol

TNFR1/TNF-α and mitochondria interrelated signaling pathway mediates quinocetone-induced apoptosis in HepG2 cells.

Authors - Zhang Chaoming C
human, mouseWB, IHC

J Exp Clin Cancer Res

Huachansu suppresses human bladder cancer cell growth through the Fas/Fasl and TNF- alpha/TNFR1 pathway in vitro and in vivo.

Authors - Tao Yang
mouseWB

Biol Reprod

Nicotine Induced Murine Spermatozoa Apoptosis via Up-Regulation of Deubiquitinated RIP1 by Trim27 Promoter Hypomethylation.

Authors - Dongsheng Nie