TNFR1 Monoclonal antibody

TNFR1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

2A6E3

Cat no : 60192-1-Ig

Synonyms

CD120a, FPF, p55, p55 R, p60, TBP1, TBPI, TNF R, TNF R I, TNF R1, TNF R55, TNF Receptor 1, TNF Receptor I, TNF RI, TNFAR, TNFR I, TNFR1, TNFR55, TNFR60, TNFRSF1A

---

Validation Data Gallery

• 

  WB analysis of HL-60 using 60192-1-Ig

  HL-60 cells, pig brain tissue, rat brain tissue and human brain tissue were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 60192-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 60192-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 60192-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 60192-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 60192-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60192-1-Ig (TNFR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 60192-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60192-1-Ig (TNFR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60192-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60192-1-Ig (TNFR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60192-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60192-1-Ig (TNFR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 60192-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TNFR1 antibody (60192-1-Ig, Clone: 2A6E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of Raji using 60192-1-Ig

  1X10^6 Raji cells were stained with 0.2ug TNFR1 antibody (60192-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

---

Tested Applications

• Positive WB detected in: HL-60 cells, Daudi cells, human brain tissue, Raji cells
• Positive IHC detected in: human liver cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human liver cancer tissue
• Positive FC detected in: Raji cells

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:2500-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 60192-1-Ig is TNFR1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TNFR1 fusion protein Ag16112
• Full Name: tumor necrosis factor receptor superfamily, member 1A
• Calculated molecular weight: 455 aa, 50 kDa
• Observed molecular weight: 50-55 kDa
• GenBank accession number: BC010140
• Gene symbol: TNFRSF1A
• Gene ID (NCBI): 7132
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.

Publications

Species	Application	Title
human	WB	Oncotarget; Inflammatory factor TNF-α promotes the growth of breast cancer via the positive feedback loop of TNFR1/NF-κB (and/or p38)/p-STAT3/HBXIP/TNFR1.; Authors - Xiaoli Cai
human	IHC	Transl Androl Urol; An experimental model of the epithelial to mesenchymal transition and pro-fibrogenesis in urothelial cells related to bladder pain syndrome/interstitial cystitis; Authors - Xing-Wei Jin
		Inflammation; Progranulin Promotes Regeneration of Inflammatory Periodontal Bone Defect in Rats via Anti-inflammation, Osteoclastogenic Inhibition, and Osteogenic Promotion.; Authors - Qian Chen
mouse	IF	Life Sci; Comparative transcriptome and Lipidome analyses suggest a lipid droplet-specific response to heat exposure of brown adipose tissue in normal and obese mice.; Authors - Qiankun Wang
human	WB	J Transl Med; CRISPR screening of E3 ubiquitin ligases reveals Ring Finger Protein 185 as a novel tumor suppressor in glioblastoma repressed by promoter hypermethylation and miR-587.; Authors - Kun Lin

Related Products

TNFR1 Polyclonal antibody

21574-1-AP

TNF Alpha Monoclonal antibody

60291-1-Ig

TNF Alpha Polyclonal antibody

17590-1-AP

TNF Beta Polyclonal antibody

13111-1-AP

TNFR2 Polyclonal antibody

19272-1-AP

FADD Polyclonal antibody

14906-1-AP

NF-κB p65 Polyclonal antibody

10745-1-AP

TRADD Polyclonal antibody

15468-1-AP