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TNFR2 Antibody 12 Publications

Rabbit Polyclonal| Catalog number: 19272-1-AP

Featured Product KD/KO validated

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Con: 20 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

TNFR2 Antibody WB Jurkat cells 19272-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 19272-1-AP(TNFR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

TNFR2 Antibody FC HeLa cells 19272-1-AP

1X10^6 HeLa cells were stained with 0.5ug TNFR2 antibody (19272-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

TNFR2 Antibody IF Hela cells 19272-1-AP

Immunofluorescent analysis of Hela cells, using TNFRSF1B antibody 19272-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

TNFR2 Antibody IP HEK-293 cells 19272-1-AP

IP Result of anti-TNFR2 (IP:19272-1-AP, 4ug; Detection:19272-1-AP 1:500) with HEK-293 cells lysate 1200ug.

TNFR2 Antibody IHC human spleen tissue 19272-1-AP

Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19272-1-AP( TNFR2 Antibody) at dilution of 1:200 (under 10x lens).

TNFR2 Antibody IHC human spleen tissue 19272-1-AP

Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19272-1-AP( TNFR2 Antibody) at dilution of 1:200 (under 40x lens).

Species specificity:
human, mouse, rat
Positive WB detected in:
Jurkat cells, HEK-293 cells, MCF-7 cells, mouse thymus tissue
Positive IP detected in:
HEK-293 cells
Positive IHC detected in:
human spleen tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Hela cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
TNFR2 fusion protein Ag5866
Full name:
tumor necrosis factor receptor superfamily, member 1B
Calculated molecular weight:
48 kDa
Observed molecular weight:
75 kDa
GenBank accession number:
BC052977
Gene ID (NCBI):
7133
Gene symbol
TNFRSF1B
Synonyms
CD120b, Etanercept, p75, p75TNFR, p80 TNF alpha receptor, TBP 2, TBPII, TNF R II, TNF R2, TNF R75, TNF Receptor 2, TNF Receptor II, TNF RII, TNFBR, TNFR II, TNFR1B, TNFR2, TNFR80, TNFRSF1B

Background

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).


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