TNFR2 Polyclonal antibody

• Featured Product
• KD/KO Validated

TNFR2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

26

Cat no : 19272-1-AP

Synonyms

CD120b, Etanercept, p75, p75TNFR, p80 TNF alpha receptor, TBP 2, TBPII, TNF R II, TNF R2, TNF R75, TNF Receptor 2, TNF Receptor II, TNF RII, TNFBR, TNFR II, TNFR1B, TNFR2, TNFR80, TNFRSF1B

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Validation Data Gallery

• 

  WB analysis of Jurkat using 19272-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19272-1-AP (TNFR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19272-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19272-1-AP (TNFR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19272-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19272-1-AP (TNFR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 19272-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19272-1-AP (TNFR2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 19272-1-AP

  IP Result of anti-TNFR2 (IP:19272-1-AP, 4ug; Detection:19272-1-AP 1:500) with HEK-293 cells lysate 1200ug.

• 

  IHC staining of human spleen using 19272-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19272-1-AP (TNFR2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 19272-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19272-1-AP (TNFR2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 19272-1-AP

  Immunofluorescent analysis of Hela cells, using TNFRSF1B antibody 19272-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 19272-1-AP

  1X10^6 HeLa cells were stained with 0.5ug TNFR2 antibody (19272-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

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Tested Applications

• Positive WB detected in: Jurkat cells, MCF-7 cells, HEK-293 cells, mouse thymus tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 17 publications below
• IHC: See 8 publications below
• IF: See 10 publications below
• FC: See 1 publications below

Product Information

The immunogen of 19272-1-AP is TNFR2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNFR2 fusion protein Ag5866
• Full Name: tumor necrosis factor receptor superfamily, member 1B
• Calculated molecular weight: 48 kDa
• Observed molecular weight: 70-75 kDa
• GenBank accession number: BC052977
• Gene symbol: TNFRSF1B
• Gene ID (NCBI): 7133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).

Publications

Species	Application	Title
human	FC,IF	Nat Immunol; NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.; Authors - Di Huang
human	WB	Mol Ther; Tumor necrosis factor alpha delivers exogenous inflammation-related microRNAs to recipient cells with functional targeting capabilities.; Authors - Yuechao Zhao
human	WB	Cancer Lett; Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells.; Authors - Bei Wang
mouse	IF	Pain; Nox2-dependent signaling between macrophages and sensory neurons contributes to neuropathic pain hypersensitivity.; Authors - Wiebke Kallenborn-Gerhardt
rat	WB	J Neuroinflammation; Gene expression comparison reveals distinct basal expression of HOX members and differential TNF-induced response between brain- and spinal cord-derived microvascular endothelial cells.; Authors - Yves Molino
human	WB,IF	J Cell Mol Med; TNFα-induced abnormal activation of TNFR/NF-κB/FTH1 in endometrium is involved in the pathogenesis of early spontaneous abortion.; Authors - Yuting Wen

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