TNFR2/CD120b Polyclonal antibody

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  WB analysis using 19272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19272-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19272-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19272-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19272-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19272-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 19272-1-AP

  IP result of anti-TNFR2 / TNFRSF1B (IP:19272-1-AP, 4ug; Detection:19272-1-AP 1:500) with HEK-293 cells lysate 1200ug.

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  IHC staining of human spleen using 19272-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19272-1-AP (TNFR2 / TNFRSF1B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 19272-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19272-1-AP (TNFR2 / TNFRSF1B Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of THP-1 using 19272-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TNFR2 / TNFRSF1B antibody (19272-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

NFR2/CD120b Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 19272-1-AP

Publications(40)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

TNFR2 / TNFRSF1B, TNFRSF1B, CD120b, Etanercept, p75

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Tested Applications

• Positive WB detected in: NK-92 cells, Jurkat cells, HEK-293 cells, THP-1 cells, mouse thymus tissue, rat thymus tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 28 publications below
• IHC: See 9 publications below
• IF: See 13 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

19272-1-AP targets NFR2/CD120b in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NFR2/CD120b fusion protein Ag5866
• Full Name: tumor necrosis factor receptor superfamily, member 1B
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC052977
• Gene Symbol: TNFR2
• Gene ID (NCBI): 7133
• RRID: AB_10640674
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20333
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).

Publications

Species	Application	Title
human	IF	Nat Immunol; NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.; Authors - Di Huang
human	WB	Mol Ther; Tumor necrosis factor alpha delivers exogenous inflammation-related microRNAs to recipient cells with functional targeting capabilities.; Authors - Yuechao Zhao
rat	WB	Mater Today Bio; Intracellular hydrogelation of macrophage conjugated probiotics for hitchhiking delivery and combined treatment of colitis; Authors - Jingzhe Wang
rat	WB	Oxid Med Cell Longev; Protective Effects of Cinnamaldehyde against Mesenteric Ischemia-Reperfusion-Induced Lung and Liver Injuries in Rats.; Authors - Marwan Almoiliqy
human	WB	Cancer Lett; Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells.; Authors - Bei Wang
mouse	IF	Pain; Nox2-dependent signaling between macrophages and sensory neurons contributes to neuropathic pain hypersensitivity.; Authors - Wiebke Kallenborn-Gerhardt

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tinne (Verified Customer) (08-14-2023)

This worked really well for ICC of human HPCs.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Human hippocampal progenitor cells

NFR2/CD120b Antibody Immunofluorescence validation (1:400 dilution) in Human hippocampal progenitor cells (Cat no:19272-1-AP)