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TNFR2 Polyclonal antibody

TNFR2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 19272-1-AP


CD120b, Etanercept, p75, p75TNFR, p80 TNF alpha receptor, TBP 2, TBPII, TNF R II, TNF R2, TNF R75, TNF Receptor 2, TNF Receptor II, TNF RII, TNFBR, TNFR II, TNFR1B, TNFR2, TNFR80, TNFRSF1B

Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells, HEK-293 cells, mouse thymus tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19272-1-AP is TNFR2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNFR2 fusion protein Ag5866
Full Name tumor necrosis factor receptor superfamily, member 1B
Calculated molecular weight 48 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC052977
Gene symbol TNFRSF1B
Gene ID (NCBI) 7133
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).


Product Specific Protocols
WB protocol for TNFR2 antibody 19272-1-APDownload protocol
IHC protocol for TNFR2 antibody 19272-1-APDownload protocol
IF protocol for TNFR2 antibody 19272-1-APDownload protocol
IP protocol for TNFR2 antibody 19272-1-APDownload protocol
FC protocol for TNFR2 antibody 19272-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Thorac Cancer

Expression of tumor necrosis factor receptor 2 in human non-small cell lung cancer and its role as a potential prognostic biomarker.

Authors - Yan Wen Zhang

BMC Mol Cell Biol

EphB4/ TNFR2/ERK/MAPK signaling pathway comprises a signaling axis to mediate the positive effect of TNF-α on osteogenic differentiation.

Authors - Yu Zhang

J Exp Clin Cancer Res

KIAA1522 potentiates TNFα-NFκB signaling to antagonize platinum-based chemotherapy in lung adenocarcinoma.

Authors - Boshi Wang

Oxid Med Cell Longev

Protective Effects of Cinnamaldehyde against Mesenteric Ischemia-Reperfusion-Induced Lung and Liver Injuries in Rats.

Authors - Marwan Almoiliqy

Front Microbiol

In vitro Evaluation of the Safety of Adalimumab for the Eye Under HTLV-1 Infection Status: A Preliminary Study.

Authors - Hisako Kurozumi-Karube

Hum Pathol

High expression of tumor necrosis factor receptor 2 in tissue is associated with progression and prognosis of esophageal squamous cell carcinoma.

Authors - Zifeng Liu