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  • KD/KO Validated

TNFR2 / TNFRSF1B Polyclonal antibody

TNFR2 / TNFRSF1B Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 19272-1-AP


CD120b, Etanercept, p75, p75TNFR, p80 TNF alpha receptor, TBP 2, TBPII, TNF R II, TNF R2, TNF R75, TNF Receptor 2, TNF Receptor II, TNF RII, TNFBR, TNFR II, TNFR1B, TNFR2, TNFR80, TNFRSF1B

Tested Applications

Positive WB detected inNK-92 cells, Jurkat cells, HEK-293 cells, THP-1 cells, mouse thymus tissue, rat thymus tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.05 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19272-1-AP targets TNFR2 / TNFRSF1B in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNFR2 / TNFRSF1B fusion protein Ag5866
Full Name tumor necrosis factor receptor superfamily, member 1B
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC052977
Gene Symbol TNFRSF1B
Gene ID (NCBI) 7133
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).


Product Specific Protocols
WB protocol for TNFR2 / TNFRSF1B antibody 19272-1-APDownload protocol
IHC protocol for TNFR2 / TNFRSF1B antibody 19272-1-APDownload protocol
IF protocol for TNFR2 / TNFRSF1B antibody 19272-1-APDownload protocol
IP protocol for TNFR2 / TNFRSF1B antibody 19272-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tinne (Verified Customer) (08-14-2023)

This worked really well for ICC of human HPCs.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: Human hippocampal progenitor cells
TNFR2 / TNFRSF1B Antibody Immunofluorescence validation (1:400 dilution) in Human hippocampal progenitor cells (Cat no:19272-1-AP)