Cardiac Troponin I Polyclonal antibody

Cardiac Troponin I Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21652-1-AP

Synonyms

Cardiac troponin I, cTnI, RCM1, TNNC1, TNNI3, troponin I type 3 (cardiac), Troponin I, cardiac muscle



Tested Applications

Positive WB detected inrat heart tissue, mouse heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse heart tissue, human heart tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21652-1-AP is Cardiac Troponin I Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cardiac Troponin I fusion protein Ag16365
Full Name troponin I type 3 (cardiac)
Calculated molecular weight 210 aa, 24 kDa
Observed molecular weight 24-26 kDa
GenBank accession numberBC096165
Gene symbol TNNI3
Gene ID (NCBI) 7137
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Troponin I (TnI) is the inhibitory subunit of troponin; the thin filament regulatory complex that confers calcium sensitivity to striated muscle actomyosin ATPase activity. Cardiac Troponin I (cTnI) is encoded by the TNNI3 gene located on chromosome 19 in humans.

What is the molecular weight of cTnI?

The molecular weight of cTnI is 24 kDa.

What is the tissue specificity of cTnI?

cTnI is produced exclusively by cardiac muscle.

What are the post-translational modifications of cTnI?

cTnI at Ser-23 and Ser-24 under adrenergic stimulation enhances the relaxation of cardiac muscle.

What is the role of cTnI in disease?

cTnI is considered to be one of the most specific and sensitive markers of myocardial damage such as acute myocardial infarction (AMI), as detection of cTnI in serum is widely accepted as a diagnostic tool (PMID: 15452154). Defects in TNNI3 lead to cardiomyopathy familial hypertrophic type 7 (CMH7); an inherited heart disorder characterized by ventricular hypertrophy (PMID: 8205619). Defects in TNNI3 can also cause cardiomyopathy familial restrictive type I (RCM1) (PMID: 20617149) and cardiomyopathy dilated type 2A (CMD2A) (PMID: 12531876). These disorders are characterized by the impaired filling of ventricles or ventricular dilation respectively.


Protocols

Product Specific Protocols
WB protocol for Cardiac Troponin I antibody 21652-1-APDownload protocol
IHC protocol for Cardiac Troponin I antibody 21652-1-APDownload protocol
IF protocol for Cardiac Troponin I antibody 21652-1-APDownload protocol
IP protocol for Cardiac Troponin I antibody 21652-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Theranostics

Localized injection of miRNA-21-enriched extracellular vesicles effectively restores cardiac function after myocardial infarction.

Authors - Yu Song
ratWB

Cell Death Dis

Exosomes derived from cardiac progenitor cells attenuate CVB3-induced apoptosis via abrogating the proliferation of CVB3 and modulating the mTOR signaling pathways.

Authors - Xin Li
humanIF

EBioMedicine

Elevated myocardial SORBS2 and the underlying implications in left ventricular noncompaction cardiomyopathy.

Authors - Chunyan Li
mouseELISA

Front Cell Dev Biol

CD38 Causes Autophagic Flux Inhibition and Cardiac Dysfunction Through a Transcriptional Inhibition Pathway Under Hypoxia/Ischemia Conditions.

Authors - Xingyue Zhang
ratWB

Cell Physiol Biochem

PGC-1α Participates in the Protective Effect of Chronic Intermittent Hypobaric Hypoxia on Cardiomyocytes.

Authors - Shuo Gu
mouseIF

EBioMedicine

Microtubule associated protein 4 phosphorylation leads to pathological cardiac remodeling in mice.

Authors - Lingfei Li