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Cardiac Troponin I Polyclonal antibody

Cardiac Troponin I Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 21652-1-AP


Cardiac troponin I, CMD2A, CMH7, cTnI, RCM1, TNNC1, TNNI3, troponin I type 3 (cardiac), Troponin I, cardiac muscle

Tested Applications

Positive WB detected inrat heart tissue, HEK-293 cells, mouse heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse heart tissue, human heart tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21652-1-AP targets Cardiac Troponin I in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cardiac Troponin I fusion protein Ag16365
Full Name troponin I type 3 (cardiac)
Calculated Molecular Weight 210 aa, 24 kDa
Observed Molecular Weight 24-26 kDa
GenBank Accession NumberBC096165
Gene Symbol TNNI3
Gene ID (NCBI) 7137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Troponin I (TnI) is the inhibitory subunit of troponin; the thin filament regulatory complex that confers calcium sensitivity to striated muscle actomyosin ATPase activity. Cardiac Troponin I (cTnI) is encoded by the TNNI3 gene located on chromosome 19 in humans.

What is the molecular weight of cTnI?

The molecular weight of cTnI is 24 kDa.

What is the tissue specificity of cTnI?

cTnI is produced exclusively by cardiac muscle.

What are the post-translational modifications of cTnI?

cTnI at Ser-23 and Ser-24 under adrenergic stimulation enhances the relaxation of cardiac muscle.

What is the role of cTnI in disease?

cTnI is considered to be one of the most specific and sensitive markers of myocardial damage such as acute myocardial infarction (AMI), as detection of cTnI in serum is widely accepted as a diagnostic tool (PMID: 15452154). Defects in TNNI3 lead to cardiomyopathy familial hypertrophic type 7 (CMH7); an inherited heart disorder characterized by ventricular hypertrophy (PMID: 8205619). Defects in TNNI3 can also cause cardiomyopathy familial restrictive type I (RCM1) (PMID: 20617149) and cardiomyopathy dilated type 2A (CMD2A) (PMID: 12531876). These disorders are characterized by the impaired filling of ventricles or ventricular dilation respectively.


Product Specific Protocols
WB protocol for Cardiac Troponin I antibody 21652-1-APDownload protocol
IHC protocol for Cardiac Troponin I antibody 21652-1-APDownload protocol
IF protocol for Cardiac Troponin I antibody 21652-1-APDownload protocol
IP protocol for Cardiac Troponin I antibody 21652-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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