TOM70 Monoclonal antibody

TOM70 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a


Human, Mouse, Rat, Pig and More (2)







Cat no : 66593-1-Ig


KIAA0719, KIAA0719, TOM70, TOM70, TOMM70A

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66593-1-Ig targets TOM70 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TOM70 fusion protein Ag6027
Full Name translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
Calculated Molecular Weight 67 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC052994
Gene Symbol TOMM70A
Gene ID (NCBI) 9868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOM70 is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer.


Product Specific Protocols
WB protocol for TOM70 antibody 66593-1-IgDownload protocol
IHC protocol for TOM70 antibody 66593-1-IgDownload protocol
IF protocol for TOM70 antibody 66593-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex.

Authors - Qiang Wang

Mol Cell

Dynamic Regulation of Mitochondrial Import by the Ubiquitin System.

Authors - Lilian Phu

Physiol Rep

TOM complex-independent transport pathway of myoglobin into mitochondria in C2C12 myotubes

Authors - Rikuhide Koma

Asian J Androl

Caseinolytic mitochondrial matrix peptidase X is essential for homologous chromosome synapsis and recombination during meiosis of male mouse germ cells

Authors - Hai-Wei Feng
  • KO Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Daniela (Verified Customer) (10-04-2022)

Good specific antibody that shows only one band at 75KDa in HeLa cell lysate (15ug/lane).

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa
TOM70 Antibody Western Blot,Immunoprecipitation validation (1:1000 dilution) in HeLa (Cat no:66593-1-Ig)