TOM70 Monoclonal antibody

TOM70 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1H8A1

Cat no : 66593-1-Ig

Synonyms

KIAA0719, KIAA0719, TOM70, TOM70, TOMM70A



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, MCF-7 cells, human brain tissue, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66593-1-Ig targets TOM70 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TOM70 fusion protein Ag6027
Full Name translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
Calculated molecular weight 67 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC052994
Gene symbol TOMM70A
Gene ID (NCBI) 9868
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOM70 is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer.

Protocols

Product Specific Protocols
WB protocol for TOM70 antibody 66593-1-IgDownload protocol
IHC protocol for TOM70 antibody 66593-1-IgDownload protocol
IF protocol for TOM70 antibody 66593-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Dynamic Regulation of Mitochondrial Import by the Ubiquitin System.

Authors - Lilian Phu