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TP53 Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 21891-1-AP

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Con: 85 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

TP53 Antibody IHC human prostate cancer tissue 21891-1-AP

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 21891-1-AP (TP53 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TP53 Antibody WB A431 cells 21891-1-AP

WB result of p53 antibody (21891-1-AP, 1:1000) with sh-control and sh-p53 transfected A431 cells.

TP53 Antibody WB A431 cells 21891-1-AP

A431 cells were subjected to SDS PAGE followed by western blot with 21891-1-AP(TP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

TP53 Antibody IP A431 cells 21891-1-AP

IP Result of anti-TP53 (IP:21891-1-AP, 3ug; Detection:21891-1-AP 1:1000) with A431 cells lysate 1650ug.

TP53 Antibody IF HepG2 cells 21891-1-AP

Immunofluorescent analysis of HepG2 cells using 21891-1-AP(TP53 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
A431 cells, HEK-293 cells, NIH/3T3 cells
Positive IP detected in:
A431 cells
Positive IHC detected in:
human prostate cancer tissue, human ovary tumor tissue, human endometrial cancer tissue
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
TP53 fusion protein Ag16596
Full name:
tumor protein p53
Calculated molecular weight:
393aa,44 kDa
Observed molecular weight:
53 kDa
GenBank accession number:
BC003596
Gene ID (NCBI):
7157
Gene symbol
TP53
Synonyms
Antigen NY CO 13, Cellular tumor antigen p53, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

Background

TP53, also named as P53 and NY-CO-13, belongs to the p53 family that functions as a central mediator for organizing cellresponses to various stress and anticancer drugswith apoptosis,G1- phase arrest, and DNA repair.[PMID: 20346922]. In addition, TP53 appears to induce apoptosis through nontranscriptional cytoplasmic processes. In unstressed cells, TP53 is kept inactive essentially through the actions of the ubiquitin ligase MDM2, which inhibits TP53 transcriptional activity and ubiquitinates TP53 to promote its degradation[PMID: 22330136]. TP53 acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)


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