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P53 Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 21891-1-AP

Featured Product KD/KO validated

1 customer review

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Con: 85 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

P53 Antibody IHC human prostate cancer tissue 21891-1-AP

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21891-1-AP (TP53 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

P53 Antibody WB A431 cells 21891-1-AP

WB result of p53 antibody (21891-1-AP, 1:1000) with sh-control and sh-p53 transfected A431 cells.

P53 Antibody WB A431 cells 21891-1-AP

A431 cells were subjected to SDS PAGE followed by western blot with 21891-1-AP(TP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

P53 Antibody IP A431 cells 21891-1-AP

IP Result of anti-TP53 (IP:21891-1-AP, 3ug; Detection:21891-1-AP 1:1000) with A431 cells lysate 1650ug.

P53 Antibody IF HepG2 cells 21891-1-AP

Immunofluorescent analysis of HepG2 cells using 21891-1-AP(TP53 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
A431 cells, HEK-293 cells, NIH/3T3 cells
Positive IP detected in:
A431 cells
Positive IHC detected in:
human prostate cancer tissue, human ovary tumor tissue, human endometrial cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
P53 fusion protein Ag16596
Full name:
tumor protein p53
Calculated molecular weight:
393aa,44 kDa
Observed molecular weight:
53 kDa
GenBank accession number:
BC003596
Gene ID (NCBI):
7157
Gene symbol
TP53
Synonyms
Antigen NY CO 13, Cellular tumor antigen p53, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

Background

1.       What is p53?

P53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2.

2.       FAQs and p53

a.       I fail to detect p53 by western blotting

Basal levels of wild-type p53 in untreated cells can be low. Try to load more cell lysate and use a positive control – a lysate of cells treated with DNA-damaging agents should increase p53 levels.

b.      I fail to detect p53 in some cell lines by western blotting

Various p53 mutations are present in cancer cell types. If mutations cause truncations/deletions some monoclonal antibodies may no longer recognize mutated p53. You have more chances of detecting various p53 mutants with our polyclonal antibody.

c.       I can detect more than one band ~50 kDa size / different cell lines give bands at slightly different size

p53 is a subject of post-translational modifications (http://p53.free.fr/p53_info/p53_modifications.html) and more than one isoform may be expressed (http://p53.free.fr/p53_info/p53_isoforms.html). Also, it is possible that your cell line of interest expresses one allele with mutated p53 with altered molecular weight.


Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

P53 Rabbit Polyclonal antibody

Y (Verified Customer), 04-01-2019
Applications
Immunohistochemistry,
Primary Antibody Dilution
200
No image submitted with review

No image submitted with review

Lot Number
00045164
We really like the trial size, it lets us try to test if the antibody works on our experiment.

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