TPI1 Polyclonal antibody

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TPI1 Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Cat No. 10713-1-AP Copied!

Publications (63)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF, FC (Intra), IP, CoIP, ELISA

Synonyms

EC:4.2.3.3, EC:5.3.1.1, Methylglyoxal synthase, TIM, TPI

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  WB analysis using 10713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HEK-293 using 10713-1-AP

  WB result of TPI1 antibody (10713-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPI1 transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat liver using 10713-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat kidney using 10713-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of RAW 264.7 using 10713-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 10713-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 10713-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 10713-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 10713-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 10713-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 10713-1-AP

  IP result of anti-TPI1 (IP:10713-1-AP, 3ug; Detection:10713-1-AP 1:1000) with HEK-293 cells lysate 2500ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HeLa using 10713-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TPI1 Polyclonal antibody (10713-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, HepG2 cells, rat liver tissue, rat kidney tissue, RAW 264.7 cells, mouse liver tissue, mouse kidney tissue, NIH/3T3 cells
• Positive IP detected in: HEK-293 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10713-1-AP targets TPI1 in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, yeast, drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1175

  Product name: Recombinant human TPI1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-249 aa of BC007086

  Sequence: MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ

• Full Name: triosephosphate isomerase 1
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC007086
• Gene Symbol: TPI1
• Gene ID (NCBI): 7167
• RRID: AB_2207716
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P60174
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316).

Publications

What published studies show

The anti-TPI1 antibody (Cat# 10713-1-AP) has accumulated 63 citations. Key journals include Cell, Nature Communications, Nature Metabolism, Nature Cell Biology, Cancer Discovery, EMBO Journal, Brain, and Cell Death & Differentiation. Research fields span glycolysis and metabolic reprogramming, cancer (colorectal, breast, ovarian, lung, pancreatic, hepatocellular), ferroptosis, the Warburg effect, stem cell biology, neurodegeneration, immunology, and reproductive biology.

Automatically generated from the 63 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell; The histone deacetylase Sirt6 regulates glucose homeostasis via Hif1alpha.; Authors - Zhong Lei L
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
mouse,human	IP,IF	Cancer Discov; LKB1-Dependent Regulation of TPI1 Creates a Divergent Metabolic Liability between Human and Mouse Lung Adenocarcinoma; Authors - Benjamin D Stein
	WB	Nat Metab; An enolase inhibitor for the targeted treatment of ENO1-deleted cancers.; Authors - Yu-Hsi Lin
human	WB	Nat Cell Biol; AMPK-regulated glycerol excretion maintains metabolic crosstalk between reductive and energetic stress; Authors - Xuewei Zhai
		Cancer Res; Exon Skipping of Ste20-Like Kinase Enhances Glycolysis and Tumor Progression by Activating Enolase 1-Mediated Phosphoenolpyruvate Production; Authors - Zeyu Chen