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TPPII Monoclonal antibody

TPPII Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF, FC

Conjugate

Unconjugated

CloneNo.

5H6F11

Cat no : 66017-1-Ig

Synonyms

TPP 2, TPP2, TPPII, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, tripeptidyl peptidase II



Tested Applications

Positive WB detected inHela cells, Jurkat
Positive IHC detected inhuman testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, MCF-7 cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 66017-1-Ig is TPPII Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TPPII fusion protein Ag5296
Full Name tripeptidyl peptidase II
Calculated molecular weight 1249 aa, 138 kDa
Observed molecular weight 135 kDa
GenBank accession numberBC039905
Gene symbol TPP2
Gene ID (NCBI) 7174
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).

Protocols

Product Specific Protocols
WB protocol for TPPII antibody 66017-1-IgDownload protocol
IHC protocol for TPPII antibody 66017-1-IgDownload protocol
IF protocol for TPPII antibody 66017-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Immunol

Different Antigen-Processing Activities in Dendritic Cells, Macrophages, and Monocytes Lead to Uneven Production of HIV Epitopes and Affect CTL Recognition.

Authors - Jens Dinter