• Featured Product
  • KD/KO Validated

TPT1 Monoclonal antibody

TPT1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1G6A10

Cat no : 66713-1-Ig

Synonyms

FLJ27337, Fortilin, Histamine releasing factor, HRF, p02, p23, TCTP, TPT1



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, MCF-7 cells, RAW 264.7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66713-1-Ig targets TPT1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TPT1 fusion protein Ag27651
Full Name tumor protein, translationally-controlled 1
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC003352
Gene symbol TPT1
Gene ID (NCBI) 7178
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22 kDa TPT1 in lysates.

Protocols

Product Specific Protocols
WB protocol for TPT1 antibody 66713-1-IgDownload protocol
IHC protocol for TPT1 antibody 66713-1-IgDownload protocol
IF protocol for TPT1 antibody 66713-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols