TRAF6 Recombinant antibody

• 

  WB analysis using 83535-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83535-2-RR (TRAF6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 83535-2-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83535-2-RR (TRAF6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 83535-2-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83535-2-RR (TRAF6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TRAF6 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 83535-2-RR

Clone No.230430H3

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

230430H3, E3 ubiquitin-protein ligase TRAF6, EC:2.3.2.27, Interleukin-1 signal transducer, RING-type E3 ubiquitin transferase TRAF6

---

Tested Applications

• Positive WB detected in: K-562 cells, HEK-293T cells, HeLa cells, Jurkat cells
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83535-2-RR targets TRAF6 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TRAF6 fusion protein Ag14858
• Full Name: TNF receptor-associated factor 6
• Calculated Molecular Weight: 522 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC031052
• Gene Symbol: TRAF6
• Gene ID (NCBI): 7189
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y4K3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.