TRAPPC12 Polyclonal antibody

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  WB analysis using 24555-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24555-1-AP (TRAPPC12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 24555-1-AP

  WB result of TRAPPC12 antibody (24555-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC12 transfected K-562 cells.

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  IP experiment of K-562 using 24555-1-AP

  IP result of anti-TRAPPC12 (IP:24555-1-AP, 4ug; Detection:24555-1-AP 1:500) with K-562 cells lysate 1720 ug.

• Featured Product
• KD/KO Validated

TRAPPC12 Polyclonal Antibody for WB, IP, ELISA

Cat No. 24555-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

TTC15, CGI 87, CGI-87, TPR repeat protein 15, Trafficking of membranes and mitosis

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IP detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24555-1-AP targets TRAPPC12 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAPPC12 fusion protein Ag18332
• Full Name: tetratricopeptide repeat domain 15
• Calculated Molecular Weight: 735 aa, 79 kDa
• Observed Molecular Weight: 98 kDa
• GenBank Accession Number: BC014164
• Gene Symbol: TRAPPC12
• Gene ID (NCBI): 51112
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WVT3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAPPC12, also called CGI-87, TTC15 and TRAMM, has been recently identified as subunit of TRAPP complex in two independent proteomic studies. It was suggested that TRAPPC12 was a subunit of TRAPPIII. TRAPPC12 and TECPR1 acted at distinct steps in autophagy and depletion of TRAPPC12 and/or TECPR1 increased in the number of autophagosomes and increase autophagic flux. The observed molecular weight of TRAPPC12 is 98 kDa, which is consistent with what has been described in the literature (PMID: 28240221, PMID: 38439956).