Validation Data Gallery
|Positive WB detected in||mouse skeletal muscle tissue, mouse heart tissue|
|Positive IHC detected in||human heart tissue, human skeletal muscle tissue, mouse heart tissue, rat brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
55456-1-AP targets TRIM63 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Full Name||tripartite motif-containing 63|
|Calculated molecular weight||40 kDa|
|Observed molecular weight||40 kDa, 35 kDa|
|GenBank accession number||NM_032588|
|Gene ID (NCBI)||84676|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
TRIM63, also named as IRF, MURF1, RNF28 and SMRZ, is E3 ubiquitin ligase. It is regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. TRIM63 may play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. It may regulate the organization of myofibrils through TTN in muscle cells. This antibody is specific to TRIM63.
Br J Pharmacol
Hydrogen Sulfide Regulates Muscle RING Finger-1 Protein S-Sulfhydration at Cys44 to Prevent Cardiac Structural Damage in Diabetic Cardiomyopathy.
Chem Biol Interact
Indoxyl sulfate induces myotube atrophy by ROS-ERK and JNK-MAFbx cascades.
J Agric Food Chem
Spermidine-Activated Satellite Cells Are Associated with Hypoacetylation in ACVR2B and Smad3 Binding to Myogenic Genes in Mice.
Age-dependent effects of caloric restriction on mTOR and ubiquitin-proteasome pathways in skeletal muscles.
J Anim Sci Biotechnol
Protective effects of taurine against muscle damage induced by diquat in 35 days weaned piglets.
J Nutr Sci Vitaminol (Tokyo)
Fish Protein Promotes Skeletal Muscle Hypertrophy via the Akt/mTOR Signaling Pathways.