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  • KD/KO Validated

TRIM63 Polyclonal antibody

TRIM63 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (4)





Cat no : 55456-1-AP


IRF, Iris RING finger protein, MuRF 1, MURF1, MURF2, RING finger protein 28, RNF28, SMRZ, TRIM63, tripartite motif containing 63

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle tissue
Positive IHC detected inhuman heart tissue, mouse skeletal muscle tissue, rat brain tissue, mouse heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55456-1-AP targets TRIM63 in WB,IHC,IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, zebrafish, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name tripartite motif-containing 63
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberNM_032588
Gene Symbol TRIM63
Gene ID (NCBI) 84676
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM63, also named as IRF, MURF1, RNF28 and SMRZ, is E3 ubiquitin ligase. It is regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. TRIM63 may play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. It may regulate the organization of myofibrils through TTN in muscle cells. TRIM63 is in muscle-specific expression.


Product Specific Protocols
WB protocol for TRIM63 antibody 55456-1-APDownload protocol
IHC protocol for TRIM63 antibody 55456-1-APDownload protocol
IF protocol for TRIM63 antibody 55456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Extracell Vesicles

Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.

Authors - Xiaohan Gao

J Cachexia Sarcopenia Muscle

Sarcopenia is attenuated by TRB3 knockout in aging mice via the alleviation of atrophy and fibrosis of skeletal muscles.

Authors - Guo-Kai Shang

J Cachexia Sarcopenia Muscle

Pim1 knockout alleviates sarcopenia in aging mice via reducing adipogenic differentiation of PDGFRα+ mesenchymal progenitors.

Authors - Guo-Kai Shang

J Cachexia Sarcopenia Muscle

Serum insulin-like growth factor binding protein 2 levels as biomarker for pancreatic ductal adenocarcinoma-associated malnutrition and muscle wasting.

Authors - Jie Dong

Cell Rep

PMID: 29972780

Authors - Ravi K Singh

Redox Biol

Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor.

Authors - Yuehong Wang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Brice-Emmanuel (Verified Customer) (10-12-2023)

I don't have the band at 40 kDa

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Mouse Heart Tissue
TRIM63 Antibody Western Blot validation (1/1000 dilution) in Mouse Heart Tissue (Cat no:55456-1-AP)