Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissue, mouse heart tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55456-1-AP targets TRIM63 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, canine, chicken, zebrafish, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | tripartite motif-containing 63 |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | NM_032588 |
| Gene Symbol | TRIM63 |
| Gene ID (NCBI) | 84676 |
| RRID | AB_11232209 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q969Q1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIM63, also named as IRF, MURF1, RNF28 and SMRZ, is E3 ubiquitin ligase. It is regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. TRIM63 may play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. It may regulate the organization of myofibrils through TTN in muscle cells. TRIM63 is in muscle-specific expression.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRIM63 antibody 55456-1-AP | Download protocol |
| IHC protocol for TRIM63 antibody 55456-1-AP | Download protocol |
| WB protocol for TRIM63 antibody 55456-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRIM63 (MuRF1) Polyclonal antibody (Cat# 55456-1-AP) has 152 citations. These appear across journals including J Cachexia Sarcopenia Muscle, Metabolism, Free Radic Biol Med, Phytomedicine, J Ethnopharmacol, Front Pharmacol, Sci Rep, J Cell Mol Med, FASEB J, and Cell Signal. Research fields center on skeletal muscle atrophy, sarcopenia, and cancer cachexia, along with muscle metabolism, diabetes, obesity, and related signaling pathways (e.g., mTOR, AMPK, ubiquitin-proteasome). Applications are predominantly Western blot, with some IHC, IF, and IP.
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway. | ||
Metabolism Anti-sarcopenic effects of active vitamin D through modulation of anabolic and catabolic signaling pathways in human skeletal muscle: A randomized controlled trial | ||
Metabolism Folic acid mitigation of alcohol-induced sarcopenia via gut-muscle axis modulation. | ||
Redox Biol Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor. | ||
Adv Sci (Weinh) Burn-Induced Gut Microbiota Dysbiosis Aggravates Skeletal Muscle Atrophy by Tryptophan-Kynurenine Mediated AHR Pathway Activation | ||
Adv Sci (Weinh) In Vivo Reprogramming of Tissue-Derived Extracellular Vesicles for Treating Chronic Tissue Injury Through Metabolic Engineering |
Reviews
What customers say
The single review for this product reports a Western Blot experiment performed on mouse heart tissue at a 1/1000 dilution. The user noted the absence of the expected 40 kDa band, resulting in a low rating. Overall, the feedback suggests the antibody did not detect the anticipated target in this particular application and tissue type.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Brice-Emmanuel (Verified Customer) (10-12-2023) | I don't have the band at 40 kDa
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