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  • KD/KO Validated

TRIM63 Polyclonal antibody

TRIM63 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 55456-1-AP

Synonyms

IRF, Iris RING finger protein, MuRF 1, MURF1, MURF2, RING finger protein 28, RNF28, SMRZ, TRIM63, tripartite motif containing 63



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, mouse heart tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue, mouse heart tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

55456-1-AP targets TRIM63 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name tripartite motif-containing 63
Calculated molecular weight 40 kDa
Observed molecular weight 40 kDa, 35 kDa
GenBank accession numberNM_032588
Gene symbol TRIM63
Gene ID (NCBI) 84676
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM63, also named as IRF, MURF1, RNF28 and SMRZ, is E3 ubiquitin ligase. It is regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. TRIM63 may play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. It may regulate the organization of myofibrils through TTN in muscle cells. This antibody is specific to TRIM63.

Protocols

Product Specific Protocols
WB protocol for TRIM63 antibody 55456-1-APDownload protocol
IHC protocol for TRIM63 antibody 55456-1-APDownload protocol
IF protocol for TRIM63 antibody 55456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Br J Pharmacol

Hydrogen Sulfide Regulates Muscle RING Finger-1 Protein S-Sulfhydration at Cys44 to Prevent Cardiac Structural Damage in Diabetic Cardiomyopathy.

Authors - Xiaojiao Sun
  • KD Validated
mouseWB

Chem Biol Interact

Indoxyl sulfate induces myotube atrophy by ROS-ERK and JNK-MAFbx cascades.

Authors - Chih-Ying Changchien
mouseWB

J Agric Food Chem

Spermidine-Activated Satellite Cells Are Associated with Hypoacetylation in ACVR2B and Smad3 Binding to Myogenic Genes in Mice.

Authors - Luchu Zhang
ratWB

Geroscience

Age-dependent effects of caloric restriction on mTOR and ubiquitin-proteasome pathways in skeletal muscles.

Authors - Chiao-Nan Chen
pigWB

J Anim Sci Biotechnol

Protective effects of taurine against muscle damage induced by diquat in 35 days weaned piglets.

Authors - Chaoyue Wen
pigWB

Res Vet Sci

Heat stress during late pregnancy of sows influences offspring longissimus dorsi muscle growth at weaning.

Authors - Yanfei Li