TRNT1 Polyclonal antibody

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  WB analysis using 31906-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31906-1-AP (TRNT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 31906-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRNT1 antibody (31906-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

TRNT1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 31906-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Mitochondrial tRNA nucleotidyl transferase, CCA-adding, EC:2.7.7.72, CGI-47, CCA-Adding Enzyme, CCA1

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31906-1-AP targets TRNT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRNT1 fusion protein Ag36149
• Full Name: tRNA nucleotidyl transferase, CCA-adding, 1
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC012537
• Gene Symbol: TRNT1
• Gene ID (NCBI): 51095
• RRID: AB_3670139
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q96Q11
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

tRNA nucleotidyl transferase 1 (TRNT1, also known as CCA1, RPEM, SIFD, MtCCA, and CGI-47 ) is a cytosine-cytosine-adenine (CCA) - adding enzyme which belongs to the tRNA nucleotidyltransferase/poly (A) polymerase family. It can catalyze the addition of the conserved nucleotide triplet CCA to the 3' terminus of tRNA molecules (PMID: 27370603). Mutations in TRNT1 would cause congenital sideroblastic anemia with immunodeficiency, fevers, and developmental delay (SIFD) (PMID: 25193871).