TRNT1 Polyclonal antibody, PBS Only

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  WB analysis using 31906-1-AP (same clone as 31906-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31906-1-AP (TRNT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31906-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 31906-1-AP (same clone as 31906-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRNT1 antibody (31906-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31906-1-PBS in a different storage buffer formulation.

TRNT1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 31906-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

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Product Information

31906-1-PBS targets TRNT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRNT1 fusion protein Ag36149
• Full Name: tRNA nucleotidyl transferase, CCA-adding, 1
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC012537
• Gene Symbol: TRNT1
• Gene ID (NCBI): 51095
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q96Q11
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

tRNA nucleotidyl transferase 1 (TRNT1, also known as CCA1, RPEM, SIFD, MtCCA, and CGI-47 ) is a cytosine-cytosine-adenine (CCA) - adding enzyme which belongs to the tRNA nucleotidyltransferase/poly (A) polymerase family. It can catalyze the addition of the conserved nucleotide triplet CCA to the 3' terminus of tRNA molecules (PMID: 27370603). Mutations in TRNT1 would cause congenital sideroblastic anemia with immunodeficiency, fevers, and developmental delay (SIFD) (PMID: 25193871).