TRPA1 Polyclonal antibody, PBS Only

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  WB analysis using 19124-1-AP (same clone as 19124-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19124-1-AP (TRPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19124-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 19124-1-AP (same clone as 19124-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19124-1-AP (TRPA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19124-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 19124-1-AP (same clone as 19124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19124-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 19124-1-AP (same clone as 19124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19124-1-PBS in a different storage buffer formulation.

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  IHC staining of rat dorsal root ganglion using 19124-1-AP (same clone as 19124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 19124-1-AP (same clone as 19124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 19124-1-AP (same clone as 19124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19124-1-PBS in a different storage buffer formulation.

TRPA1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 19124-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

ANKTM1, p120, Wasabi receptor

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Product Information

19124-1-PBS targets TRPA1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: transient receptor potential cation channel, subfamily A, member 1
• Calculated Molecular Weight: 140 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: NM_007332
• Gene Symbol: TRPA1
• Gene ID (NCBI): 8989
• RRID: AB_10642143
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75762
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TRPA1, also named as ANKTM1, belongs to the transient receptor family. TRPA1 is a receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. TRPA1 has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. It acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. It may be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds.