TRPC4 Recombinant antibody

• 

  WB analysis using 85334-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85334-2-RR (TRPC4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

TRPC4 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 85334-2-RR

Clone No.242444F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85334-2-RR targets TRPC4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TRPC4 fusion protein Ag15698
• Full Name: transient receptor potential cation channel, subfamily C, member 4
• Calculated Molecular Weight: 977 aa, 112 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC104725
• Gene Symbol: TRPC4
• Gene ID (NCBI): 7223
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UBN4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRPC4 (Transient Receptor Potential Paradigm 4) is a non-selective calcium channel protein widely found in the nervous system and cardiovascular system. TRPC4 regulates intracellular calcium levels through the activation of signaling pathways mediated by Gq/11 and Gi/o-coupled receptors, and is involved in neurotransmission, neuronal excitability, and vascular endothelial cell function. In the nervous system, TRPC4 is associated with neuropsychiatric disorders such as depression and anxiety, and its inhibitors show antidepressant and anxiolytic potential. In the cardiovascular system, TRPC4 affects vascular permeability and diastolic function by regulating calcium ion inward flow and plays an important role in hypoxia-induced pulmonary hypertension. In addition, TRPC4 is involved in thermoregulation, sensing changes in internal temperature to regulate the drop in body temperature. aberrant expression of TRPC4 is associated with a variety of diseases, making it a potential therapeutic target!