Tested Applications
| Positive WB detected in | mouse lung tissue, COLO 320 cells, mouse brain tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human placenta tissue |
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18236-1-AP targets TRPC6 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12976 Product name: Recombinant human TRPC6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-405 aa of BC093658 Sequence: MSQSPAFGPRRGSSPRGAAGAAARRNESQDYLLMDSELGEDGCPQAPLPCYGYYPCFRGSDNRLAHRRQTVLREKGRRLANRGPAYMFSDRSTSLSIEEERFLDAAEYGNIPVVRKMLEECHSLNVNCVDYMGQNALQLAVANEHLEITELLLKKENLSRVGDALLLAISKGYVRIVEAILSHPAFAEGKRLATSPSQSELQQDDFYAYDEDGTRFSHDVTPIILAAHCQEYEIVHTLLRKGARIERPHDYFCKCNDCNQKQKHDSFSHSRSRINAYKGLASPAYLSLSSEDPVMTALELSNELAVLANIEKEFKNDYKKLSMQCKDFVVGLLDLCRNTEEVEAILNGDVETLQSGDHGRPNLSRLKLAIKYEVKKFVAHPNCQQQLLSIWYENLSGLRQQTMAV Predict reactive species |
| Full Name | transient receptor potential cation channel, subfamily C, member 6 |
| Calculated Molecular Weight | 931 aa, 106 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC093658 |
| Gene Symbol | TRPC6 |
| Gene ID (NCBI) | 7225 |
| RRID | AB_10859822 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y210 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transient receptor potential canonical (TRPC) channels mediate the influx of different types of cations through the cell membrane and are involved in many functions of the organism (PMID: 28508319). The TRPC subfamily consists of seven members, TRPC1, TRPC2, TRPC3, TRPC4, TRPC5, TRPC6, TRPC7 (PMID: 32872338). TRPC6 is a Ca(2+)-permeable non-selective cation channel expressed in most human tissues. TRPC6 has been considered as potential therapeutic targets for malignant glioma treatment (PMID: 35489424).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for TRPC6 antibody 18236-1-AP | Download protocol |
| IF protocol for TRPC6 antibody 18236-1-AP | Download protocol |
| IHC protocol for TRPC6 antibody 18236-1-AP | Download protocol |
| IP protocol for TRPC6 antibody 18236-1-AP | Download protocol |
| WB protocol for TRPC6 antibody 18236-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRPC6 polyclonal antibody (Cat# 18236-1-AP) has 32 citations across journals including Cell, Cancer Cell, Nature Biomedical Engineering, PNAS, Biomaterials, Cell Reports, Kidney International Reports, and others. Research fields span calcium channel biology, pulmonary hypertension, kidney disease (diabetic nephropathy, fibrosis), neurology (neuropathic pain, hearing loss, glioma, cerebral ischemia), cardiovascular disease, cancer immunotherapy, epigenetics, and pharmacology. Cited applications include WB, IF, IHC, and IP across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Cancer Cell Tumor-infiltrating plasma cell profiling after PD-1 blockade reveals tumor-specific antibodies. | ||
Nat Biomed Eng Staple oligomers induce a stable RNA G-quadruplex structure for protein translation inhibition in therapeutics. | ||
J Nanobiotechnology Hypoxia-injured pulmonary vascular targeting and microenvironment-responsive nanoparticle to treat hypoxic pulmonary hypertension. | ||
Biomaterials Nongenetic optical modulation of neural stem cell proliferation and neuronal/glial differentiation. | ||
Phytomedicine Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro |
