- Featured Product
- KD/KO Validated
TRPC6 Polyclonal antibody
TRPC6 Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 18236-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain, COLO 320 cells, mouse lung tissue |
Positive IP detected in | mouse lung tissue |
Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 5 publications below |
IF | See 5 publications below |
IP | See 1 publications below |
FC | See 1 publications below |
Product Information
The immunogen of 18236-1-AP is TRPC6 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TRPC6 fusion protein Ag12976 |
Full Name | transient receptor potential cation channel, subfamily C, member 6 |
Calculated molecular weight | 931 aa, 106 kDa |
Observed molecular weight | 100-110 kDa |
GenBank accession number | BC093658 |
Gene symbol | TRPC6 |
Gene ID (NCBI) | 7225 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for TRPC6 antibody 18236-1-AP | Download protocol |
IHC protocol for TRPC6 antibody 18236-1-AP | Download protocol |
IP protocol for TRPC6 antibody 18236-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biomaterials Nongenetic optical modulation of neural stem cell proliferation and neuronal/glial differentiation. | ||
Proc Natl Acad Sci U S A TRPC6 channel translocation into phagosomal membrane augments phagosomal function.
| ||
Br J Pharmacol Catalpol Alleviates Adriamycin-Induced Nephropathy by Activating the SIRT1 Signalling Pathway In Vivo and In Vitro. | ||
Lab Invest Amelioration of circadian disruption and calcium-handling protein defects by choline alleviates cardiac remodeling in abdominal aorta coarctation rats. | ||
Arch Biochem Biophys TRPC6 interacted with KCa1.1 channels to regulate the proliferation and apoptosis of glioma cells.
|