TRPC6 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRPC6 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 18236-1-AP

Synonyms

FSGS2, Transient receptor protein 6, TRP 6, TRP6, TRPC6

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Validation Data Gallery

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  WB analysis of mouse brain using 18236-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500.

• 

  WB analysis of mouse lung using 18236-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 18236-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 18236-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 18236-1-AP

  IP Result of anti-TRPC6 (IP:18236-1-AP, 4ug; Detection:18236-1-AP 1:300) with mouse lung tissue lysate 3600ug.

• 

  IHC staining of human placenta using 18236-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18236-1-AP (TRPC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 18236-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18236-1-AP (TRPC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of A549 using 18236-1-AP

  1X10^6 A549 cells were stained with 0.2ug TRPC6 antibody (18236-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: mouse brain, COLO 320 cells, mouse lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Product Information

The immunogen of 18236-1-AP is TRPC6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRPC6 fusion protein Ag12976
• Full Name: transient receptor potential cation channel, subfamily C, member 6
• Calculated molecular weight: 931 aa, 106 kDa
• Observed molecular weight: 100-110 kDa
• GenBank accession number: BC093658
• Gene symbol: TRPC6
• Gene ID (NCBI): 7225
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	FC	Cell; Regenerative Metaplastic Clones in COPD Lung Drive Inflammation and Fibrosis.; Authors - Wei Rao
mouse	WB,IF	Biomaterials; Nongenetic optical modulation of neural stem cell proliferation and neuronal/glial differentiation.; Authors - Meitian Wang
human	IF	Proc Natl Acad Sci U S A; TRPC6 channel translocation into phagosomal membrane augments phagosomal function.; Authors - Vladimir Riazanski ; KO Validated
mouse	WB	Br J Pharmacol; Catalpol Alleviates Adriamycin-Induced Nephropathy by Activating the SIRT1 Signalling Pathway In Vivo and In Vitro.; Authors - Jiangnan Zhang
rat	WB	Lab Invest; Amelioration of circadian disruption and calcium-handling protein defects by choline alleviates cardiac remodeling in abdominal aorta coarctation rats.; Authors - Xi He
rat	WB,IF,IP	Arch Biochem Biophys; TRPC6 interacted with KCa1.1 channels to regulate the proliferation and apoptosis of glioma cells.; Authors - Hongqiang Yin ; KD Validated

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