|Positive IHC detected in||human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
20987-1-AP targets TRPV4 in IHC applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||transient receptor potential cation channel, subfamily V, member 4|
|Calculated molecular weight||98 kDa|
|GenBank accession number||NM_021625|
|Gene ID (NCBI)||59341|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TRPV4, also named as VRL2 and VROAC, belongs to the transient receptor family and TrpV subfamily. It is a non-selective calcium permeant cation channel protein which involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. It is activated by low pH, citrate and phorbol esters. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Defects in TRPV4 are the cause of brachyolmia type 3 (BRAC3), spondylometaphyseal dysplasia Kozlowski type (SMDK) and metatropic dysplasia (MTD). Multiple transcript variants encoding different isoforms have been found.
Significance of transient receptor potential vanilloid 4 and aquaporin 5 co-expression in the rat uterus at term.
Decrease in AQP4 expression level in atrophied skeletal muscles with innervation.
Expressions of TRPVs in the cholesteatoma epithelium.
Cell Mol Bioeng
The mechanosensitive ion channel TRPV4 is a regulator of lung development and pulmonary vasculature stabilization.
TRPV4 inhibitor HC067047 produces antidepressant-like effect in LPS-induced depression mouse model.
Phosphodiesterase 3B (PDE3B) regulates NLRP3 inflammasome in adipose tissue.