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TRPV4 Polyclonal antibody

TRPV4 Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC,ELISA

Conjugate

Unconjugated

Cat no : 20987-1-AP

Synonyms

Osm 9 like TRP channel 4, OTRPC4, TRP12, TRPV4, VR OAC, VRL 2, VRL2, VROAC



Tested Applications

Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20987-1-AP targets TRPV4 in IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name transient receptor potential cation channel, subfamily V, member 4
Calculated molecular weight 98 kDa
GenBank accession numberNM_021625
Gene symbol TRPV4
Gene ID (NCBI) 59341
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRPV4, also named as VRL2 and VROAC, belongs to the transient receptor family and TrpV subfamily. It is a non-selective calcium permeant cation channel protein which involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. It is activated by low pH, citrate and phorbol esters. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Defects in TRPV4 are the cause of brachyolmia type 3 (BRAC3), spondylometaphyseal dysplasia Kozlowski type (SMDK) and metatropic dysplasia (MTD). Multiple transcript variants encoding different isoforms have been found.

Protocols

Product Specific Protocols
IHC protocol for TRPV4 antibody 20987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Heliyon

Significance of transient receptor potential vanilloid 4 and aquaporin 5 co-expression in the rat uterus at term.

Authors - Eszter Ducza
ratWB

Physiol Rep

Decrease in AQP4 expression level in atrophied skeletal muscles with innervation.

Authors - Minenori Ishido
humanIF

Acta Otolaryngol

Expressions of TRPVs in the cholesteatoma epithelium.

Authors - Ba Hung Do

Cell Mol Bioeng

The mechanosensitive ion channel TRPV4 is a regulator of lung development and pulmonary vasculature stabilization.

Authors - Joshua T Morgan
mouseWB

Sci Rep

Phosphodiesterase 3B (PDE3B) regulates NLRP3 inflammasome in adipose tissue.

Authors - Faiyaz Ahmad
mouseIHC

Environ Pollut

Exposure to both formaldehyde and high relative humidity exacerbates allergic asthma by activating the TRPV4-p38 MAPK pathway in Balb/c mice.

Authors - Jiufei Duan