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- KD/KO Validated
TSG101 Polyclonal antibody
TSG101 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, RIP, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 14497-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Hela cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 90 publications below |
| IHC | See 3 publications below |
| IF | See 4 publications below |
| IP | See 1 publications below |
| RIP | See 1 publications below |
Product Information
14497-1-AP targets TSG101 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TSG101 fusion protein Ag5920 |
| Full Name | tumor susceptibility gene 101 |
| Calculated molecular weight | 44 kDa |
| Observed molecular weight | 46 kDa |
| GenBank accession number | BC002487 |
| Gene symbol | TSG101 |
| Gene ID (NCBI) | 7251 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TSG101 antibody 14497-1-AP | Download protocol |
| IHC protocol for TSG101 antibody 14497-1-AP | Download protocol |
| IF protocol for TSG101 antibody 14497-1-AP | Download protocol |
| IP protocol for TSG101 antibody 14497-1-AP | Download protocol |
| FC protocol for TSG101 antibody 14497-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EBioMedicine A GBM-like V-ATPase signature directs cell-cell tumor signaling and reprogramming via large oncosomes. | ||
Int J Biochem Cell Biol Exosomes from hypoxia-treated human adipose-derived mesenchymal stem cells enhance angiogenesis through VEGF/VEGF-R. | ||
Stem Cells Transl Med Impaired Bone Regenerative Effect of Exosomes Derived from Bone Marrow Mesenchymal Stem Cells in Type 1 Diabetes. | ||
J Biol Chem Modulation of calreticulin expression reveals a novel exosome mediated mechanism of Z variant alpha-1 antitrypsin disposal. | ||
Int J Biol Macromol Exosomal miR-1229 derived from colorectal cancer cells promotes angiogenesis by targeting HIPK2. | ||
Stem Cell Res Ther Exosomes secreted by mesenchymal stromal/stem cell-derived adipocytes promote breast cancer cell growth via activation of Hippo signaling pathway. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Pasquale (Verified Customer) (11-06-2019) | The antibody recognize the exosomal marker TSG101 as claimed. We tested it by Western blotting in our exosome preps isolated from murine brains and it gave the signal at the correct molecular weight in the fractions that are enriched in exosomes, so it seems to be specific. However, we report here some drawbacks that we have experienced using this antibody (see the file attached): 1. There is a strong cross-reactivity of the antibody with the molecular weight marker we use (The Bio-Rad Dual Color Standard, cat. number 1610374) 2. The signal over noise ratio is a little low (the background coming from the membrane is pretty high)
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