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TSG101 Antibody 18 Publications

Rabbit Polyclonal| Catalog number: 14497-1-AP

Featured Product KD/KO validated

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Con: 57 μg/150 μl

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Tested Applications
Applications:
ELISA, FC, IF, IHC, IP, WB
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Images See all

14497-1-AP;HeLa cells

HeLa cells were subjected to SDS PAGE followed by western blot with 14497-1-AP(TSG101 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

14497-1-AP;Hela cells

Immunofluorescent analysis of Hela cells, using TSG101 antibody 14497-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

14497-1-AP;HeLa cells

IP Result of anti-TSG101 (IP:14497-1-AP, 3ug; Detection:14497-1-AP 1:500) with HeLa cells lysate 1320ug.

14497-1-AP;human colon cancer tissue

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 14497-1-AP( TSG101 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

14497-1-AP;human colon cancer tissue

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 14497-1-AP( TSG101 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

14497-1-AP;HeLa cells

1X10^6 HeLa cells were stained with 0.2ug TSG101 antibody (14497-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells, NIH/3T3 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human colon cancer tissue, human colon cancer tissue, human heart tissue
Positive IF detected in:
Hela cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:100-1:400
IF : 1:10-1:100

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
TSG101 fusion protein ag5920
Full name:
tumor susceptibility gene 101
Calculated molecular weight:
44 kDa
Observed molecular weight:
46 kDa
GenBank accession number:
BC002487
Gene ID (NCBI):
7251
Gene symbol
TSG101
Synonyms
ESCRT I complex subunit TSG101, TSG10, TSG101, tumor susceptibility gene 101, VPS23
Background

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing.


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