HeLa cells were subjected to SDS PAGE followed by western blot with 14497-1-AP(TSG101 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Various lysates were subjected to SDS PAGE followed by western blot with 14497-1-AP (TSG101 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14497-1-AP(TSG101 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 14497-1-AP( TSG101 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 14497-1-AP( TSG101 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical of paraffin-embedded human heart using 14497-1-AP(TSG101 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human heart using 14497-1-AP(TSG101 antibody) at dilution of 1:50 (under 40x lens)

IP Result of anti-TSG101 (IP:14497-1-AP, 3ug; Detection:14497-1-AP 1:500) with HeLa cells lysate 1320ug.

Immunofluorescent analysis of Hela cells, using TSG101 antibody 14497-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

1X10^6 HeLa cells were stained with 0.2ug TSG101 antibody (14497-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.