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TSG101 Antibody 37 Publications

Rabbit Polyclonal| Catalog number: 14497-1-AP

Featured Product KD/KO validated

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Con: 61 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

TSG101 Antibody WB NIH/3T3 cells 14497-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 14497-1-AP (TSG101 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

TSG101 Antibody IP HeLa cells 14497-1-AP

IP Result of anti-TSG101 (IP:14497-1-AP, 3ug; Detection:14497-1-AP 1:500) with HeLa cells lysate 1320ug.

TSG101 Antibody IHC human colon cancer tissue 14497-1-AP

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14497-1-AP( TSG101 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TSG101 Antibody IHC human colon cancer tissue 14497-1-AP

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14497-1-AP( TSG101 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TSG101 Antibody IF Hela cells 14497-1-AP

Immunofluorescent analysis of Hela cells, using TSG101 antibody 14497-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

TSG101 Antibody FC HeLa cells 14497-1-AP

1X10^6 HeLa cells were stained with 0.2ug TSG101 antibody (14497-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
NIH/3T3 cells, HeLa cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human colon cancer tissue, human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Hela cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:1000-1:5000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:100-1:400
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
TSG101 fusion protein Ag5920
Full name:
tumor susceptibility gene 101
Calculated molecular weight:
44 kDa
Observed molecular weight:
46 kDa
GenBank accession number:
BC002487
Gene ID (NCBI):
7251
Gene symbol
TSG101
Synonyms
ESCRT I complex subunit TSG101, TSG10, TSG101, tumor susceptibility gene 101, VPS23

Background

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.


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