|Positive WB detected in||HeLa cells, mouse ovary tissue, mouse thymus tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
14450-1-AP targets TSHR in WB, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TSHR fusion protein Ag5957|
|Full Name||thyroid stimulating hormone receptor|
|Calculated molecular weight||87 kDa|
|Observed molecular weight||62-70 kDa|
|GenBank accession number||BC063613|
|Gene ID (NCBI)||7253|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Thyroid stimulating hormone (also known as thyrotropin or TSH) is a glycoprotein hormone produced by thyrotrophs in the anterior pituitary gland. The TSH receptor (TSHR), mainly expressed on the thyroid epithelial cells, is a G protein-coupled receptor (GPCR) of the seven-transmembrane domain family and plays a central role in controlling thyroid cell metabolism. The gene of TSHR maps to chromosome 14q31.1, and encodes a 764-amino acid protein with a predicted unmodified molecular weight of 86.8 kDa. The apparent molecular weight of glycosylated form is 95-120 kDa. Studies reveal that TSHR is composed of two subunits, A and B, which are produced by cleavage of single-chain TSHR on the cell surface and subsequently connected by disulfide bonds. This rabbit polyclonal antibody raised against N-terminal region (1-253 aa) of human TSHR can detect subunit A with an experimentally determined molecular weight of 62-70 kDa under reducing condition. (PMID: 20578897, 17158770, 12593718)
Front Endocrinol (Lausanne)
Long Non-coding Antisense RNA TNRC6C-AS1 Is Activated in Papillary Thyroid Cancer and Promotes Cancer Progression by Suppressing TNRC6C Expression.
J Cell Mol Med
Combined tazemetostat and MAPKi enhances differentiation of papillary thyroid cancer cells harbouring BRAFV600E by synergistically decreasing global trimethylation of H3K27.
Am J Cancer Res
A somatic mutation of RasGRP3 decreases Na+/I- symporter expression in metastases of radioactive iodine-refractory thyroid cancer by stimulating the Akt signaling pathway.
Dominant negative effect of mutated thyroid stimulating hormone receptor (P556L) causes hypothyroidism in C.RF-Tshr(hyt/wild) mice.
J Transl Med
Serum and thyroid tissue level of let-7b and their correlation with TRAb in Graves' disease.
HER inhibitor promotes BRAF/MEK inhibitor-induced redifferentiation in papillary thyroid cancer harboring BRAFV600E.