TSHR Polyclonal antibody

TSHR Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 14450-1-AP

Synonyms

CHNG1, hTSHR I, LGR3, Thyrotropin receptor, TSH R, TSHR



Tested Applications

Positive WB detected inHeLa cells, mouse ovary tissue, mouse thymus tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

14450-1-AP targets TSHR in WB, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSHR fusion protein Ag5957
Full Name thyroid stimulating hormone receptor
Calculated molecular weight 87 kDa
Observed molecular weight 62-70 kDa
GenBank accession numberBC063613
Gene symbol TSHR
Gene ID (NCBI) 7253
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Thyroid stimulating hormone (also known as thyrotropin or TSH) is a glycoprotein hormone produced by thyrotrophs in the anterior pituitary gland. The TSH receptor (TSHR), mainly expressed on the thyroid epithelial cells, is a G protein-coupled receptor (GPCR) of the seven-transmembrane domain family and plays a central role in controlling thyroid cell metabolism. The gene of TSHR maps to chromosome 14q31.1, and encodes a 764-amino acid protein with a predicted unmodified molecular weight of 86.8 kDa. The apparent molecular weight of glycosylated form is 95-120 kDa. Studies reveal that TSHR is composed of two subunits, A and B, which are produced by cleavage of single-chain TSHR on the cell surface and subsequently connected by disulfide bonds. This rabbit polyclonal antibody raised against N-terminal region (1-253 aa) of human TSHR can detect subunit A with an experimentally determined molecular weight of 62-70 kDa under reducing condition. (PMID: 20578897, 17158770, 12593718)

Protocols

Product Specific Protocols
WB protocol for TSHR antibody 14450-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Endocrinol (Lausanne)

Long Non-coding Antisense RNA TNRC6C-AS1 Is Activated in Papillary Thyroid Cancer and Promotes Cancer Progression by Suppressing TNRC6C Expression.

Authors - Dilidaer Muhanhali
humanWB

J Cell Mol Med

Combined tazemetostat and MAPKi enhances differentiation of papillary thyroid cancer cells harbouring BRAFV600E by synergistically decreasing global trimethylation of H3K27.

Authors - Hao Fu
humanWB

Am J Cancer Res

A somatic mutation of RasGRP3 decreases Na+/I- symporter expression in metastases of radioactive iodine-refractory thyroid cancer by stimulating the Akt signaling pathway.

Authors - Jianlu Song
humanWB

PLoS One

Dominant negative effect of mutated thyroid stimulating hormone receptor (P556L) causes hypothyroidism in C.RF-Tshr(hyt/wild) mice.

Authors - Endo Toyoshi T
humanWB

J Transl Med

Serum and thyroid tissue level of let-7b and their correlation with TRAb in Graves' disease.

Authors - Xinxin Chen
humanWB

Oncotarget

HER inhibitor promotes BRAF/MEK inhibitor-induced redifferentiation in papillary thyroid cancer harboring BRAFV600E.

Authors - Lingxiao Cheng