Thrombospondin 1 Antibody

Thrombospondin 1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat no : 18304-1-AP

Synonyms

THBS, THBS 1, THBS1, thrombospondin 1, TSP, TSP 1, TSP1



Tested Applications

Positive WB detected inC6 cells, Human peripheral blood platelets tissue, A431 cells, HeLa cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human placenta tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18304-1-AP targets Thrombospondin 1 in IHC, WB, ELISA applications and shows reactivity with human, rat, mouse samples.

Reactivity human, rat, mouse
Cited Species human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name thrombospondin 1
Calculated molecular weight 129 kDa
Observed molecular weight 180 kDa
GenBank accession number BC028145
Gene symbol THBS1
Gene ID (NCBI) 7057
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

TSP1, also named as THBS1, THBS and TSP, is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions, and which can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1, alpha-V/beta-3 and alpha-IIb/beta-3. TSP1 is a regulator of many biological processes including cell growth, adhesion, migration, platelet aggregation, and fibrin deposition and lysis. It interacts with a number of plasma proteins such as fibrinogen and plasminogen and co-polymerizes with fibrin in clot formation. Secreted TSP1 is a glycoprotein with a molecular mass of 150-180 kDa.

Protocols

Product Specific Protocols
WB protocol for Thrombospondin 1 antibody 18304-1-APDownload protocol
IHC protocol for Thrombospondin 1 antibody 18304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Mol Med Rep

Astaxanthin ameliorates renal interstitial fibrosis and peritubular capillary rarefaction in unilateral ureteral obstruction.

Authors - Jin Zhao
humanWB

Oral Surg Oral Med Oral Pathol Oral Radiol

Upregulation of proangiogenic factors expression in the synovium of temporomandibular joint condylar hyperplasia.

Authors - Huilin Guo
humanWB

J Oral Pathol Med

IL-1β-regulating angiogenic factors expression in perforated temporomandibular disk cells via NF-κB pathway.

Authors - Jie Xu
humanIHC

Oncotarget

Novel circulating peptide biomarkers for esophageal squamous cell carcinoma revealed by a magnetic bead-based MALDI-TOFMS assay.

Authors - Kun Jia
mouseIF

Nat Commun

Activation of E-prostanoid 3 receptor in macrophages facilitates cardiac healing after myocardial infarction.

Authors - Juan Tang