Thrombospondin 1 Polyclonal antibody

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  WB analysis using 18304-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18304-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18304-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 18304-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human peripheral blood platelets using 18304-1-AP

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 18304-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 18304-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 18304-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 18304-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 18304-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 18304-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

Thrombospondin 1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 18304-1-AP

Publications(47)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

Thrombospondin-1, THBS1, TSP, THBS 1, THBS

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Tested Applications

• Positive WB detected in: C6 cells, A431 cells, human peripheral blood platelets, HeLa cells, HUVEC cells, NIH/3T3 cells
• Positive IHC detected in: human lung cancer tissue, human placenta tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 40 publications below
• IHC: See 15 publications below
• IF: See 12 publications below

Product Information

18304-1-AP targets Thrombospondin 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: thrombospondin 1
• Calculated Molecular Weight: 129 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC028145
• Gene Symbol: Thrombospondin 1
• Gene ID (NCBI): 7057
• RRID: AB_2201959
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P07996
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thrombospondin 1(TSP-1), an extracellular matrix protein, is the first identified natural angiogenesis inhibitor. A variety of normal cells, including endothelial cells, fibroblasts, adipocytes, smooth muscle cells, monocytes, macrophages, and transformed cells such as malignant glioma cells, secrete Thrombospondin 1. It regulates cellular phenotype during tissue genesis and repair. It acts as a molecular facilitator by bringing together cytokines, growth factors, matrix components, membrane receptors and extracellular proteases. Thrombospondin 1 binds to a wide variety of integrin and non-integrin cell surface receptors. It is also a major activator of transforming growth factor (TGFβ1). Secreted TSP1 is a glycoprotein with a molecular mass of 150-180 kDa.

Publications

Species	Application	Title
human	WB	Cancer Cell; Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation.; Authors - Yansen Xiao
human	WB	Nat Commun; The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets.; Authors - Henry Nording
mouse	IF	Nat Commun; Activation of E-prostanoid 3 receptor in macrophages facilitates cardiac healing after myocardial infarction.; Authors - Juan Tang
human	IHC	Cancer Lett; Gastric cancer derived exosomal THBS1 enhanced Vγ9Vδ2 T-cell function through activating RIG-I-like receptor signaling pathway in a N6-methyladenosine methylation dependent manner; Authors - Juntao Li
rat	IF,WB	Transl Res; Ntsr1 contributes to pulmonary hypertension by enhancing endoplasmic reticulum stress via JAK2-STAT3-Thbs1 signaling; Authors - Zhi-Xing Wei
rat	WB	J Med Chem; Hypoxia Activated Nitric Oxide Donor Compounds for the Prevention and Treatment of Myocardial Hypoxia-Induced Injury; Authors - Wanxiang Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emma (Verified Customer) (03-15-2022)

A single band by WB in DU145 cells. Used at 1:1000 @ 4C overnight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Prostate