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Thrombospondin 1 Polyclonal antibody
Thrombospondin 1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 18304-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | C6 cells, HeLa cells, human peripheral blood platelets, A431 cells, NIH/3T3 cells |
Positive IHC detected in | human lung cancer tissue, human placenta tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 21 publications below |
IHC | See 7 publications below |
IF | See 4 publications below |
Product Information
18304-1-AP targets Thrombospondin 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Reactivity | human, rat, mouse |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | thrombospondin 1 |
Calculated molecular weight | 129 kDa |
Observed molecular weight | 180 kDa |
GenBank accession number | BC028145 |
Gene symbol | THBS1 |
Gene ID (NCBI) | 7057 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Thrombospondin 1(TSP-1), an extracellular matrix protein, is the first identified natural angiogenesis inhibitor. A variety of normal cells, including endothelial cells, fibroblasts, adipocytes, smooth muscle cells, monocytes, macrophages, and transformed cells such as malignant glioma cells, secrete Thrombospondin 1. It regulates cellular phenotype during tissue genesis and repair. It acts as a molecular facilitator by bringing together cytokines, growth factors, matrix components, membrane receptors and extracellular proteases. Thrombospondin 1 binds to a wide variety of integrin and non-integrin cell surface receptors. It is also a major activator of transforming growth factor (TGFβ1). Secreted TSP1 is a glycoprotein with a molecular mass of 150-180 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for Thrombospondin 1 antibody 18304-1-AP | Download protocol |
IHC protocol for Thrombospondin 1 antibody 18304-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancer Cell Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation. | ||
Nat Commun The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets. | ||
Nat Commun Activation of E-prostanoid 3 receptor in macrophages facilitates cardiac healing after myocardial infarction. | ||
Oncotarget Novel circulating peptide biomarkers for esophageal squamous cell carcinoma revealed by a magnetic bead-based MALDI-TOFMS assay. | ||
Int J Mol Sci Yth m6A RNA-Binding Protein 1 Regulates Osteogenesis of MC3T3-E1 Cells under Hypoxia via Translational Control of Thrombospondin-1 | ||
Front Oncol tRNA-Derived Fragment tRF-17-79MP9PP Attenuates Cell Invasion and Migration via THBS1/TGF-β1/Smad3 Axis in Breast Cancer.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Emma (Verified Customer) (03-15-2022) | A single band by WB in DU145 cells. Used at 1:1000 @ 4C overnight.
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