TTDN1 Polyclonal antibody

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  WB analysis of NIH/3T3 using 25410-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25410-1-AP (TTDN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 25410-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25410-1-AP (TTDN1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TTDN1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25410-1-AP

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

C7orf11, ABHS, TTD non-photosensitive 1 protein, ORF20, MPLKIP

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Tested Applications

• Positive WB detected in: NIH/3T3 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25410-1-AP targets TTDN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TTDN1 fusion protein Ag22094
• Full Name: chromosome 7 open reading frame 11
• Calculated Molecular Weight: 179 aa, 19 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC026265
• Gene Symbol: TTDN1
• Gene ID (NCBI): 136647
• RRID: AB_2935461
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TAP9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TTDN1 colocalizes with Plk1 at the centrosome in mitosis and the midbody during cytokinesis. It is phosphorylated by Cdk1 in mitosis, and this is required for its interaction with Plk1. Overexpression of TTDN1 or its knockdown by siRNA causes multi-polar spindles and multiple nuclei, suggesting that TTDN1 plays a role in regulating mitosis and cytokinesis. Mutations in the TTDN1 gene are associated with a distinct trichothiodystrophy phenotype. (PMID:17310276, 25290684)

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Moritz (Verified Customer) (08-20-2024)

I used this antibody to detect MPLKIP/TTDN1 with Western blot in HEK293T WT cell lysate, which worked perfectly.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293T WT

TTDN1 Antibody Western Blot validation (1:3000 dilution) in HEK293T WT (Cat no:25410-1-AP)

FH

Tsimafei (Verified Customer) (08-12-2024)

Antibody detect a band in the range between 20-25 kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

TTDN1 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:25410-1-AP)